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[188416_s_at]

Description T20H4.4 /REP_DB=WormBase Gene ID /WP=CE25120 /GEN=adr-1 /TR=SW:Q22618 /GB=AAA50661.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 67      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 74      6grp_total > 68      4grp_total > 55      6grp_highest_sub > Da      6grp_cgc > 38      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 279
1 2 3 4 range
A 777.2
(3)
P 		632.2
(-1.8)
P 		759.4
(2.4)
P 		596.1
(-3)
P 		181
B 818.6
(3)
P 		539.7
(-3)
P 		608.9
(-1.5)
P 		706.7
(0.6)
P 		279
C 809.2
(3)
P 		601.9
(-3)
P 		628.6
(-2.2)
P 		675.1
(-0.9)
P 		207
avr 801.7
(3) 		591.3
(-3) 		665.6
(-0.9) 		659.3
(-1.1) 		210

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 326
1 2 3 4 5 6 range
A 714.6
(1)
P 		541.4
(-2.7)
P 		526.6
(-3)
P 		656.7
(-0.3)
P 		645.9
(-0.5)
P 		811.8
(3)
P 		285
B 680.9
(-0.7)
P 		594.3
(-2.6)
P 		701.1
(-0.3)
P 		577.8
(-3)
P 		623.7
(-2)
P 		852.3
(3)
P 		275
C 625.1
(-2.4)
P 		609.1
(-2.9)
P 		604.2
(-3)
P 		710.1
(0.1)
P 		619.2
(-2.6)
P 		806.8
(3)
P 		203
avr 673.5
(-0.7) 		581.6
(-3) 		610.6
(-2.3) 		648.2
(-1.3) 		629.6
(-1.8) 		823.6
(3) 		242
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 283
(3)
P 		137.2
(-1.5)
P 		198.5
(0.4)
P 		87.3
(-3)
P 		196
B 261
(3)
P 		133.3
(-3)
P 		141.1
(-2.6)
P 		150.6
(-2.2)
P 		128
C 267.9
(3)
P 		173.1
(-0.7)
P 		219.1
(1.1)
P 		113.8
(-3)
P 		154
avr 270.6
(3) 		147.9
(-1.8) 		186.2
(-0.3) 		117.2
(-3) 		153

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 131.7
(-1.8)
P 		192.5
(2.6)
P 		114.7
(-3)
P 		166.6
(0.8)
P 		194.9
(2.8)
P 		197.7
(3)
P 		83
B 236.9
(0.1)
P 		197.8
(-1.4)
P 		170.2
(-2.4)
P 		153
(-3)
P 		253.4
(0.7)
P 		317.8
(3)
P 		165
C 231.7
(1.2)
P 		185.8
(-1)
P 		153.8
(-2.5)
P 		142.8
(-3)
P 		200.4
(-0.3)
P 		270.1
(3)
P 		127
avr 200.1
(-0.2) 		192
(-0.6) 		146.2
(-3) 		154.1
(-2.6) 		216.2
(0.6) 		261.9
(3) 		116
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188416_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7010
Sequence Type Exemplar sequence
Representative Public ID T20H4.4 NCBI
Target Description T20H4.4 /REP_DB=WormBase Gene ID /WP=CE25120 /GEN=adr-1 /TR=SW:Q22618 /GB=AAA50661.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066193(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066193 NCBI Caenorhabditis elegans Adenosine Deaminase Acting on RNA family member (adr-2) (adr-2) mRNA, complete cds. 11/11 None
SNAP00000008518 ENSEMBL cdna:SNAP chromosome:CEL140:III:7231097:7232806:-1 11/11 None
GENEFINDER00000008529 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7231097:7232806:-1 11/11 None
T20H4.4.1 ENSEMBL cdna:known chromosome:CEL140:III:7230888:7232806:-1 gene:T20H4.4 11/11 None
T20H4.4.2 ENSEMBL cdna:known chromosome:CEL140:III:7230979:7232808:-1 gene:T20H4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7231094-7232804(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol adr-2
Ensembl T20H4.4
Entrez Gene 176022 Entrez gene
SwissProt Q22618 EMBL-EBI
WormBase CE25120 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.17329.1.S1_AT similar to RNA-specific adenosine deaminase B1 isoform DRADA2a cfa
DROSOPHILA_2:1634276_A_AT Adenosine deaminase acting on RNA dm
DROSGENOME1:141675_AT Adenosine deaminase acting on RNA dm
CHICKEN:GGA.13806.1.S1_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) gga
CHICKEN:GGA.2137.1.S1_S_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) gga
HG-U133_PLUS_2:207999_S_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133A:207999_S_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133A_2:207999_S_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133_PLUS_2:209979_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133A:209979_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133A_2:209979_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U95AV2:38748_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133A:203865_S_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133_PLUS_2:203865_S_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-FOCUS:203865_S_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133A_2:203865_S_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HU35KSUBC:RC_AA262743_S_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HUGENEFL:U76421_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
U133_X3P:G7669476_3P_A_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
U133_X3P:G4501918_3P_A_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
U133_X3P:G2114492_3P_AT adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
U133_X3P:HS.287748.0.S1_3P_AT Adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133_PLUS_2:234799_AT Adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133B:234799_AT Adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133_PLUS_2:234539_AT Adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
HG-U133B:234539_AT Adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) hs
MOUSE430_2:1421480_A_AT adenosine deaminase, RNA-specific, B1 mm
MOE430A:1421480_A_AT adenosine deaminase, RNA-specific, B1 mm
MOUSE430A_2:1421480_A_AT adenosine deaminase, RNA-specific, B1 mm
MU11KSUBA:R75314_RC_AT adenosine deaminase, RNA-specific, B1 mm
MG-U74AV2:93153_AT adenosine deaminase, RNA-specific, B1 mm
MG-U74AV2:161436_S_AT adenosine deaminase, RNA-specific, B1 mm
MOE430A:1434932_AT adenosine deaminase, RNA-specific, B1 mm
MOUSE430A_2:1434932_AT adenosine deaminase, RNA-specific, B1 mm
MOUSE430_2:1434932_AT adenosine deaminase, RNA-specific, B1 mm
MOE430B:1443968_AT adenosine deaminase, RNA-specific, B1 mm
MOUSE430_2:1443968_AT adenosine deaminase, RNA-specific, B1 mm
MU19KSUBB:TC33214_AT Adenosine deaminase, RNA-specific, B1 (Adarb1), transcript variant 1, mRNA mm
RG-U34A:U43534_AT adenosine deaminase, RNA-specific, B1 rn
RAE230A:1368933_AT adenosine deaminase, RNA-specific, B1 rn
RAT230_2:1368933_AT adenosine deaminase, RNA-specific, B1 rn
RAE230B:1391888_AT adenosine deaminase, RNA-specific, B1 rn
RAT230_2:1391888_AT adenosine deaminase, RNA-specific, B1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6396 RNA processing inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
3725 double-stranded RNA binding inferred from electronic annotation QuickGO AmiGO
4000 adenosine deaminase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA50661 Adenosine deaminase that acts on rna protein 2 [Caenorhabditis elegans] ref|NP_498594.1| Adenosine Deaminase Acting on RNA family member (adr-2) [Caenorhabditis elegans] gb|AAC25097.1| putative RNA adenosine deaminase [Caenorhabditis elegans] sp|Q22618|ADR2_CAEEL Probable double-stranded RNA-specific adenosine deaminase (DRADA) 0.0
blast T16913 hypothetical protein T20H4.4 - Caenorhabditis elegans 0.0
blast CAE56450 Hypothetical protein CBG24155 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002466 EMBL-EBI Adenosine deaminase/editase 1.0E-126
Pfam IPR001159 EMBL-EBI Double-stranded RNA binding (DsRBD) domain 1.2E-12
Sequence
Target Sequence 
>C. ELEGANS:188416_S_AT
gtcttgctctgataaattgcttcgagcaaatgtactgggtgtccaaggagcaatattatc
acatttcatcgatccaatctattactcgtcaatcgccgttgccgagttgaacaatgcaga
tcgtctccgtaaagcagtttacagcagagcagccaccttcaaacctccagctccattcca
tgttcaagacgtggaaatcggagagtgtcaagtggaagatactgagcaatcaacttctgc
agcagcacgatccacgattagttcgatgaattggaacttggctgatggaaatacagaagt
tgtacgaacatcggatggaatggttcatgataaggatatgagcggggctgacatcacgac
tccatccagattgtgtaaaaaaaatatggcagaactgatgattactatctgcacgttgac
aaagacttctgtggattatccaatctcatacgaagaactcaaagctggatctcaagaata
cgctgctgcaaaaaagtcgttcattacctggctgcgacagaaggatctcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTTGCTCTGATAAATTGCTTCGA 285 469 921 Antisense
TACTCGTCAATCGCCGTTGCCGAGT 192 645 1003 Antisense
GCAGATCGTCTCCGTAAAGCAGTTT 91 313 1036 Antisense
TTTACAGCAGAGCAGCCACCTTCAA 576 671 1058 Antisense
CAGCTCCATTCCATGTTCAAGACGT 602 203 1088 Antisense
GATACTGAGCAATCAACTTCTGCAG 125 425 1138 Antisense
CAGCAGCACGATCCACGATTAGTTC 528 203 1160 Antisense
TATGAGCGGGGCTGACATCACGACT 591 655 1257 Antisense
GGATCTCAAGAATACGCTGCTGCAA 145 513 1387 Antisense
AAAAAGTCGTTCATTACCTGGCTGC 651 129 1411 Antisense
ACCTGGCTGCGACAGAAGGATCTCG 431 85 1426 Antisense
Sequence Source Affymetrix Proprietary Database