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[188410_at] protease

Description T23F4.4 /REP_DB=WormBase Gene ID /WP=CE14090 /TR=O17264 /GB=AAB71031.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=protease
Cluster 6grp_total > 71      4grp_total > 41      6grp_cgc > 86      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 10.6
(-3)
A 		136
(3)
A 		104.7
(1.5)
A 		71.7
(-0.1)
A 		125
B 8
(-3)
A 		104.9
(2.2)
A 		119.9
(3)
A 		41.6
(-1.2)
A 		112
C 11.2
(-3)
A 		119.6
(3)
A 		84.4
(1.1)
A 		20.9
(-2.5)
A 		108
avr 9.9
(-3) 		120.2
(3) 		103
(2.1) 		44.7
(-1.1) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 156
1 2 3 4 5 6 range
A 5
(-2.9)
A 		69.2
(0.6)
A 		102
(2.4)
A 		71.5
(0.7)
A 		113.9
(3)
A 		2.9
(-3)
A 		111
B 11.4
(-3)
A 		43.4
(-1.2)
A 		83.5
(1)
A 		119.5
(3)
A 		103.6
(2.1)
A 		36.3
(-1.6)
A 		108
C 11.2
(-2.8)
A 		6.8
(-3)
A 		32.5
(-2)
A 		158.7
(3)
A 		41.4
(-1.6)
A 		43.7
(-1.5)
A 		152
avr 9.2
(-3) 		39.8
(-1.3) 		72.7
(0.5) 		116.6
(3) 		86.3
(1.3) 		27.6
(-2) 		107
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 3.9
(-3)
A 		29.5
(3)
A 		27.4
(2.5)
A 		10.5
(-1.5)
A 		26
B 2.5
(-3)
A 		25.9
(2.5)
A 		27.8
(3)
A 		8.9
(-1.5)
A 		25
C 3.7
(-3)
A 		34.4
(3)
A 		29.4
(2)
A 		3.5
(-3)
A 		31
avr 3.4
(-3) 		29.9
(3) 		28.2
(2.6) 		7.6
(-2) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 0.9
(-3)
A 		24.6
(1.3)
A 		22.2
(0.8)
A 		18.1
(0.1)
A 		34.4
(3)
A 		0.7
(-3)
A 		34
B 4
(-3)
A 		14.4
(-1.4)
A 		20.3
(-0.4)
A 		31.7
(1.4)
A 		42.1
(3)
A 		13.5
(-1.5)
A 		38
C 4.2
(-2.6)
A 		2.1
(-3)
A 		8.3
(-1.8)
A 		31.9
(3)
A 		13.4
(-0.7)
A 		14.6
(-0.5)
A 		30
avr 3
(-3) 		13.7
(-0.6) 		16.9
(0.1) 		27.2
(2.4) 		30
(3) 		9.6
(-1.5) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188410_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5416
Sequence Type Exemplar sequence
Representative Public ID T23F4.4 NCBI
Target Description T23F4.4 /REP_DB=WormBase Gene ID /WP=CE14090 /TR=O17264 /GB=AAB71031.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061525(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061525 NCBI Caenorhabditis elegans T23F4.4 (protease) mRNA, complete cds. 11/11 None
SNAP00000024152 ENSEMBL cdna:SNAP chromosome:CEL140:II:1169895:1180906:-1 11/11 None
GENEFINDER00000024154 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1176078:1179157:-1 11/11 None
T23F4.4 ENSEMBL cdna:known chromosome:CEL140:II:1176078:1179329:-1 gene:T23F4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1176076-1179353(-) 100.0 100.0
Public Domain and Genome References
Gene Title protease
Gene Symbol nas-27
Ensembl T23F4.4
Entrez Gene 188809 Entrez gene
SwissProt O17264 EMBL-EBI
WormBase CE36020 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB71031 Nematode astacin protease protein 27 [Caenorhabditis elegans] ref|NP_493926.2| T23F4.4 [Caenorhabditis elegans] sp|O17264|NAS27_CAEEL Zinc metalloproteinase nas-27 precursor (Nematode astacin 27) 0.0
blast CAE62910 Hypothetical protein CBG07102 [Caenorhabditis briggsae] 0.0
blast AAB71032 Hypothetical protein T23F4.3 [Caenorhabditis elegans] ref|NP_493925.1| T23F4.3 [Caenorhabditis elegans] 1.0E-115
blast P98061 Zinc metalloproteinase nas-28 precursor (Nematode astacin 28) 9.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.5E-96
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 9.5E-90
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 5.7E-78
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 1.5E-93
Sequence
Target Sequence 
>C. ELEGANS:188410_AT
gagccacgtttcaggatgtctcgaggatgaatgtgctttataattgtcatgaacgctgtg
caaacaccctaaaccggtgtcaacaaggcggctacccggctccgagcgactgctcccagt
gtgtgtgccccgatggttttggcgggaacttttgtgagactattgaggcccattcagtag
gccagaaggataattcggattgtggaggggtgttatgggcttccgaaacatctcaaacct
tctacggagccgtgcgcaccagagtccactcgaactccgtcctaccaaccccggagcact
gtttttggcatattagagcatcccaaggaaaatccatagagatccaaatcaagaacatca
tctcaccatgctccatgtcatgctcctttaatgctctggagctcaagctgtcaaatttca
caatgacaggtcccagattctgctgcgacgagcacatctacaataggtactcccagccaa
aagtctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCCACGTTTCAGGATGTCTCGAG 367 385 836 Antisense
TGAACGCTGTGCAAACACCCTAAAC 9 575 885 Antisense
GTGTGCCCCGATGGTTTTGGCGGGA 202 487 958 Antisense
ATGGGCTTCCGAAACATCTCAAACC 362 49 1050 Antisense
CATCTCAAACCTTCTACGGAGCCGT 450 195 1064 Antisense
CCCGGAGCACTGTTTTTGGCATATT 179 261 1125 Antisense
TCAAGAACATCATCTCACCATGCTC 557 631 1184 Antisense
CATGTCATGCTCCTTTAATGCTCTG 585 209 1209 Antisense
AATGACAGGTCCCAGATTCTGCTGC 704 163 1257 Antisense
GATTCTGCTGCGACGAGCACATCTA 544 431 1271 Antisense
ATAGGTACTCCCAGCCAAAAGTCTT 527 19 1298 Antisense
Sequence Source Affymetrix Proprietary Database