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[188395_at] spectrin

Description F20C5.6 /REP_DB=WormBase Gene ID /WP=CE03243 /TR=Q18379 /GB=CAA92300.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=spectrin
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      6grp_highest_nonorm > 60      6grp_total > 07      4grp_total > 47      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 300
1 2 3 4 range
A 328.2
(3)
P 		109.5
(-1.7)
M 		49
(-3)
A 		112.3
(-1.6)
P 		279
B 277.3
(3)
P 		108.4
(-1.1)
P 		28.1
(-3)
A 		70
(-2)
P 		249
C 306.4
(3)
P 		88.6
(-2.7)
A 		76.8
(-3)
A 		134.7
(-1.5)
P 		230
avr 304
(3) 		102.2
(-1.8) 		51.3
(-3) 		105.7
(-1.7) 		253

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 604
1 2 3 4 5 6 range
A 695
(3)
P 		328.4
(-1)
P 		251.1
(-1.8)
P 		185.6
(-2.5)
P 		139.1
(-3)
P 		148
(-2.9)
P 		556
B 645.6
(3)
P 		341.1
(-0.9)
P 		289.8
(-1.6)
P 		246.2
(-2.1)
P 		179.3
(-3)
P 		179.4
(-3)
P 		466
C 742.2
(3)
P 		303.9
(-1.4)
P 		217.6
(-2.2)
P 		224
(-2.2)
P 		138.6
(-3)
P 		173.7
(-2.7)
P 		604
avr 694.3
(3) 		324.5
(-1.1) 		252.8
(-1.9) 		218.6
(-2.3) 		152.3
(-3) 		167
(-2.8) 		542
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 113
1 2 3 4 range
A 119.5
(3)
P 		23.8
(-2.4)
M 		12.8
(-3)
A 		16.4
(-2.8)
P 		107
B 88.4
(3)
P 		26.8
(-1.5)
P 		6.5
(-3)
A 		14.9
(-2.4)
P 		82
C 101.4
(3)
P 		25.5
(-2.8)
A 		26.8
(-2.7)
A 		22.7
(-3)
P 		79
avr 103.1
(3) 		25.4
(-2.3) 		15.4
(-3) 		18
(-2.8) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 239
1 2 3 4 5 6 range
A 128.1
(3)
P 		116.8
(2.3)
P 		54.7
(-1.8)
P 		47.1
(-2.3)
P 		42
(-2.6)
P 		36
(-3)
P 		92
B 224.6
(3)
P 		113.6
(-1.2)
P 		70.4
(-2.8)
P 		65.2
(-3)
P 		72.8
(-2.7)
P 		66.9
(-2.9)
P 		159
C 275.1
(3)
P 		92.7
(-1.8)
P 		55.4
(-2.7)
P 		45
(-3)
P 		44.8
(-3)
P 		58.1
(-2.7)
P 		230
avr 209.3
(3) 		107.7
(-0.9) 		60.2
(-2.7) 		52.4
(-3) 		53.2
(-3) 		53.7
(-3) 		157
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188395_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10130
Sequence Type Exemplar sequence
Representative Public ID F20C5.6 NCBI
Target Description F20C5.6 /REP_DB=WormBase Gene ID /WP=CE03243 /TR=Q18379 /GB=CAA92300.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=spectrin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069112(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069112 NCBI Caenorhabditis elegans F20C5.6 (spectrin) mRNA, complete cds. 11/11 None
F20C5.6 ENSEMBL cdna:known chromosome:CEL140:IV:8771539:8773186:-1 gene:F20C5.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000033364 6/11 Cross Hyb Matching Probes None
GENEFINDER00000033386 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8771941-8773173(-) 100.0 100.0
Public Domain and Genome References
Gene Title spectrin
Ensembl F20C5.6
Entrez Gene 177687 Entrez gene
SwissProt Q18379 EMBL-EBI
WormBase CE03243 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92300 Hypothetical protein F20C5.6 [Caenorhabditis elegans] emb|CAA92289.1| Hypothetical protein F20C5.6 [Caenorhabditis elegans] ref|NP_501513.1| F20C5.6 [Caenorhabditis elegans] 0.0
blast XP_687637 PREDICTED: similar to Golgi autoantigen, golgin subfamily A member 4 (Trans-Golgi p230) (256 kDa golgin) (Golgin-245) (72.1 protein) [Danio rerio] 2.0E-14
blast S06117 myosin heavy chain, nonmuscle (clone lambda-FMHC) - chicken (fragment) 4.0E-13
Sequence
Target Sequence 
>C. ELEGANS:188395_AT
agaactgtgctcagcggatatgaatacaggaaagatgtgtagacgtgccaaaagagcgga
agatagaattgctaaattggaatccgatcttgaagaagaacgccaaaaacatgtgggaca
agacatcagtcatattttggatcaacataactcgaccgtcatggcgttagagatgcaact
tgcaaacactcagcaggaaaaagccacgcttaatacatcagttgaagatcttaaacttcg
aactgaagagtggaagcacaacttattggggcaatttgaagtattgacagccaattcaaa
gagagaatgtgaatcaaaagaagaacagattgcaaatcataaaagagaaatcatggaaaa
agatagcaaaattgagtgtctgatggaacttattcaaaatagcgaacgatccgctgaaat
tcagaagatgcatgatgcattgagagagaaggatttgaagatttcggaactttcggaaaa
actgcaaggtcttcttatcaatcaagaaggagaagctgatgatacaatgatcgatcaaga
tgacgacaataaatccacacacagtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAACTGTGCTCAGCGGATATGAAT 199 73 453 Antisense
GGAAAGATGTGTAGACGTGCCAAAA 416 527 481 Antisense
TGGATCAACATAACTCGACCGTCAT 555 549 590 Antisense
TCGACCGTCATGGCGTTAGAGATGC 71 603 604 Antisense
GATGCAACTTGCAAACACTCAGCAG 397 413 624 Antisense
AACACTCAGCAGGAAAAAGCCACGC 408 135 637 Antisense
AAAGCCACGCTTAATACATCAGTTG 311 125 652 Antisense
GGAAGCACAACTTATTGGGGCAATT 83 531 704 Antisense
GCGAACGATCCGCTGAAATTCAGAA 231 287 854 Antisense
AAACTGCAAGGTCTTCTTATCAATC 550 125 931 Antisense
GACGACAATAAATCCACACACAGTG 607 379 994 Antisense
Sequence Source Affymetrix Proprietary Database