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[188394_at]

Description F57H12.2 /REP_DB=WormBase Gene ID /WP=CE17123 /GEN=unc-24 /TR=Q17372 /GB=AAC02604.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 23      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 80      6grp_total > 21      4grp_total > 21      6grp_highest_sub > 02      6grp_cgc > 62      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 282
1 2 3 4 range
A 227.6
(2.1)
P 		151.4
(-0.5)
P 		80.7
(-3)
P 		253.7
(3)
P 		173
B 232.8
(-0.6)
P 		180.4
(-2.1)
P 		147.2
(-3)
P 		363
(3)
P 		216
C 247.1
(1)
P 		206.8
(-1)
P 		165.8
(-3)
P 		286.3
(3)
P 		121
avr 235.8
(0.7) 		179.5
(-1.3) 		131.2
(-3) 		301
(3) 		170

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 332
1 2 3 4 5 6 range
A 231.2
(0.2)
P 		254.2
(0.8)
P 		353.6
(3)
P 		322
(2.3)
P 		226
(0.1)
P 		88.5
(-3)
P 		265
B 277.5
(0.2)
P 		420.9
(3)
P 		231
(-0.7)
P 		303.1
(0.7)
P 		209.8
(-1.1)
P 		109.7
(-3)
P 		311
C 254.4
(0.5)
P 		367.8
(3)
P 		252.3
(0.4)
P 		253.6
(0.4)
P 		180.4
(-1.2)
P 		100.7
(-3)
P 		267
avr 254.4
(0.7) 		347.6
(3) 		279
(1.3) 		292.9
(1.7) 		205.4
(-0.4) 		99.6
(-3) 		248
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 82.9
(3)
P 		32.9
(-1.9)
P 		21.1
(-3)
P 		37.1
(-1.4)
P 		62
B 74.2
(2.6)
P 		44.5
(-1.6)
P 		34.1
(-3)
P 		77.4
(3)
P 		43
C 81.8
(3)
P 		59.5
(-1)
P 		57.8
(-1.3)
P 		48.3
(-3)
P 		34
avr 79.6
(3) 		45.6
(-1.9) 		37.7
(-3) 		54.3
(-0.6) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 119
1 2 3 4 5 6 range
A 42.6
(-1.2)
P 		90.4
(3)
P 		77
(1.8)
P 		81.7
(2.2)
P 		68.2
(1.1)
P 		21.6
(-3)
P 		69
B 96.5
(0.4)
P 		140.1
(3)
P 		56.1
(-2.1)
P 		80.3
(-0.6)
P 		85.2
(-0.3)
P 		40.9
(-3)
P 		99
C 94.3
(1.6)
P 		112.2
(3)
P 		64.2
(-0.7)
P 		51
(-1.7)
P 		58.4
(-1.1)
P 		33.7
(-3)
P 		79
avr 77.8
(0.3) 		114.2
(3) 		65.8
(-0.5) 		71
(-0.2) 		70.6
(-0.2) 		32.1
(-3) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188394_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9592
Sequence Type Exemplar sequence
Representative Public ID F57H12.2 NCBI
Target Description F57H12.2 /REP_DB=WormBase Gene ID /WP=CE17123 /GEN=unc-24 /TR=Q17372 /GB=AAC02604.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068934(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068934 NCBI Caenorhabditis elegans UNCoordinated family member (unc-24) (unc-24) mRNA, complete cds. 11/11 None
SNAP00000044155 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7979797:7981931:1 11/11 None
GENEFINDER00000044162 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7979866:7981931:1 11/11 None
F57H12.2 ENSEMBL cdna:known chromosome:CEL140:IV:7979995:7981931:1 gene:F57H12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7980000-7981937(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol unc-24
Ensembl F57H12.2
Entrez Gene 177594 Entrez gene
SwissProt Q17372 EMBL-EBI
WormBase CE17123 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27302.1.S1_S_AT similar to stomatin (EPB72)-like 1 cfa
HG-U133_PLUS_2:204701_S_AT stomatin (EPB72)-like 1 hs
HG-U133A:204701_S_AT stomatin (EPB72)-like 1 hs
HG-U133A_2:204701_S_AT stomatin (EPB72)-like 1 hs
HU35KSUBB:RC_F04524_S_AT stomatin (EPB72)-like 1 hs
U133_X3P:G4759147_3P_S_AT stomatin (EPB72)-like 1 hs
HG-U133A_2:33736_AT stomatin (EPB72)-like 1 hs
HG-FOCUS:33736_AT stomatin (EPB72)-like 1 hs
HG-U133_PLUS_2:33736_AT stomatin (EPB72)-like 1 hs
HG-U133A:33736_AT stomatin (EPB72)-like 1 hs
HG-U95AV2:33736_AT stomatin (EPB72)-like 1 hs
U133_X3P:HS2.423602.1.S1_3P_AT Stomatin (EPB72)-like 1 hs
HG-U133_PLUS_2:1556320_AT Stomatin (EPB72)-like 1 hs
MG-U74CV2:131386_AT stomatin-like 1 mm
MOE430B:1452738_AT stomatin-like 1 mm
MOUSE430_2:1452738_AT stomatin-like 1 mm
RAE230B:1377918_AT similar to Stomatin-like 1 rn
RAT230_2:1377918_AT similar to Stomatin-like 1 rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5498 sterol carrier activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC02604 Uncoordinated protein 24 [Caenorhabditis elegans] ref|NP_501335.1| UNCoordinated family member (unc-24) [Caenorhabditis elegans] gb|AAB06496.1| UNC-24 0.0
blast CAE70928 Hypothetical protein CBG17728 [Caenorhabditis briggsae] 0.0
blast CAE70928 Hypothetical protein CBG17728 [Caenorhabditis briggsae] 1.0E-177
blast XP_784779 PREDICTED: similar to stomatin (EPB72)-like 1 [Strongylocentrotus purpuratus] 6.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR001107 EMBL-EBI Band 7 protein 8.5E-49
Pfam IPR003033 EMBL-EBI Sterol-binding 4.5E-41
Pfam IPR001107 EMBL-EBI Band 7 protein 6.2E-46
Pfam IPR003033 EMBL-EBI Sterol-binding 4.5E-41
Pfam IPR001107 EMBL-EBI Band 7 protein 8.5E-49
Pfam IPR003033 EMBL-EBI Sterol-binding 4.5E-41
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501335.1 1 66-88
F57H12.2 1 66-88
GENEFINDER00000044162 1 109-131
Sequence
Target Sequence 
>C. ELEGANS:188394_AT
gagctgggatcatttacgtgtcaattcggagttgaaatcactgacgtggaaatttccgat
gtgaaaattgtgaaggaaggagagaacatggggatgtctgcgttgagcagcgttgcgaaa
tccgatgctggtcaacagttgtggcaagttattggaccagtattcgaagactttgcaaag
gaatgtgcagctgaagagaaagcaaaagaaaatgcaccattagttgatttgtcggatgtt
ccctcaacttcagctgcgggaacttctacagatacaccaaatattccttccattgatatc
gaccatcttatatctgtggcaagcctagctatggatgaacacttagtccgtctaattggc
agagttttccaaattaattgcaaagatattgagcccatttgcattgatttgaaacatgga
tctggaagtgcgtataaaggaacttccttaaatcctgacgtggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCTGGGATCATTTACGTGTCAAT 486 387 643 Antisense
GGATGTCTGCGTTGAGCAGCGTTGC 499 513 734 Antisense
AGCGTTGCGAAATCCGATGCTGGTC 610 81 751 Antisense
TGATTTGTCGGATGTTCCCTCAACT 279 565 867 Antisense
AACTTCAGCTGCGGGAACTTCTACA 376 137 888 Antisense
ACAGATACACCAAATATTCCTTCCA 57 109 910 Antisense
ATTCCTTCCATTGATATCGACCATC 670 7 925 Antisense
GACCATCTTATATCTGTGGCAAGCC 595 383 943 Antisense
GAACACTTAGTCCGTCTAATTGGCA 67 351 979 Antisense
TTGAGCCCATTTGCATTGATTTGAA 464 705 1031 Antisense
GAACTTCCTTAAATCCTGACGTGGT 89 351 1082 Antisense
Sequence Source Affymetrix Proprietary Database