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[188392_s_at]

Description C32F10.2 /REP_DB=WormBase Gene ID /WP=CE24823 /GEN=lin-35 /TR=O01682 /GB=AAC24266.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 67      4grp_highest_8_8 > 35      4grp_high_8_8 > 48      6grp_highest_nonorm > 83      6grp_total > 57      4grp_total > 63      6grp_highest_sub > Cu      6grp_cgc > 28      6grp_high1_sub > Da      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 272
1 2 3 4 range
A 530.1
(3)
P 		304.5
(-2)
P 		351.7
(-0.9)
P 		258.6
(-3)
P 		272
B 377.2
(2.4)
P 		353.3
(0.6)
P 		385.8
(3)
P 		305.7
(-3)
P 		80
C 478.4
(3)
P 		292.5
(-3)
P 		413.6
(0.9)
P 		323.7
(-2)
P 		186
avr 461.9
(3) 		316.8
(-2.2) 		383.7
(0.2) 		296
(-3) 		166

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 234
1 2 3 4 5 6 range
A 370.6
(-2.7)
P 		398.8
(-1.6)
P 		374.5
(-2.6)
P 		371.3
(-2.7)
P 		363.4
(-3)
P 		516
(3)
P 		153
B 463.9
(0.1)
P 		417.8
(-1.1)
P 		347.4
(-3)
P 		358.3
(-2.7)
P 		414.2
(-1.2)
P 		571.1
(3)
P 		224
C 435.5
(-0.3)
P 		413
(-0.9)
P 		406.9
(-1.1)
P 		398.3
(-1.3)
P 		336.8
(-3)
P 		552.7
(3)
P 		216
avr 423.3
(-1.2) 		409.9
(-1.7) 		376.3
(-2.8) 		376
(-2.8) 		371.5
(-3) 		546.6
(3) 		175
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 193
(3)
P 		66.1
(-1.9)
P 		91.9
(-0.9)
P 		37.9
(-3)
P 		155
B 120.3
(3)
P 		87.3
(-0.6)
P 		89.4
(-0.4)
P 		65.1
(-3)
P 		55
C 158.4
(3)
P 		84.1
(-1.3)
P 		144.1
(2.2)
P 		54.6
(-3)
P 		104
avr 157.2
(3) 		79.2
(-1.5) 		108.5
(0.2) 		52.5
(-3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 145
1 2 3 4 5 6 range
A 68.3
(-3)
P 		141.8
(3)
P 		81.6
(-1.9)
P 		94.2
(-0.9)
P 		109.7
(0.4)
P 		125.6
(1.7)
P 		74
B 161.4
(0.6)
P 		139.1
(-0.4)
P 		84.4
(-3)
P 		94.9
(-2.5)
P 		168.3
(0.9)
P 		213
(3)
P 		129
C 161.4
(1.7)
P 		126
(-0.4)
P 		103.6
(-1.7)
P 		80.1
(-3)
P 		109
(-1.3)
P 		185
(3)
P 		105
avr 130.4
(-0.1) 		135.6
(0.2) 		89.9
(-3) 		89.7
(-3) 		129
(-0.2) 		174.5
(3) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188392_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1975
Sequence Type Exemplar sequence
Representative Public ID C32F10.2 NCBI
Target Description C32F10.2 /REP_DB=WormBase Gene ID /WP=CE24823 /GEN=lin-35 /TR=O01682 /GB=AAC24266.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059285(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059285 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-35) (lin-35) mRNA, complete cds. 11/11 A 
GENEFINDER00000021241 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5819404:5823465:1 11/11 None
SNAP00000021235 ENSEMBL cdna:SNAP chromosome:CEL140:I:5819684:5823465:1 11/11 None
C32F10.2 ENSEMBL cdna:known chromosome:CEL140:I:5815845:5823773:1 gene:C32F10.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5815865-5823465(+) 97.68 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lin-35
Ensembl C32F10.2
Entrez Gene 172249 Entrez gene
SwissProt O01682 EMBL-EBI
WormBase CE24823 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604598_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE:1592688_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE:1584401_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE_2:CFA.12061.1.A1_S_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE_2:CFA.18203.1.S1_S_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE_2:CFA.19796.1.S1_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE_2:CFAAFFX.15185.1.S1_S_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CHICKEN:GGA.7958.3.S1_AT similar to retinoblastoma-like 2 (p130) gga
CHICKEN:GGAAFFX.25929.1.S1_AT similar to retinoblastoma-like 2 (p130) gga
CHICKEN:GGAAFFX.25929.3.S1_S_AT similar to retinoblastoma-like 2 (p130) gga
HU35KSUBD:RC_F10323_F_AT retinoblastoma-like 2 (p130) hs
HG-U95AV2:1986_AT retinoblastoma-like 2 (p130) hs
HC-G110:1986_AT retinoblastoma-like 2 (p130) hs
HU35KSUBC:RC_W58597_S_AT retinoblastoma-like 2 (p130) hs
HUGENEFL:X76061_AT retinoblastoma-like 2 (p130) hs
U133_X3P:HS.79362.0.S3_3P_AT retinoblastoma-like 2 (p130) hs
U133_X3P:HS.79362.0.S1_3P_AT retinoblastoma-like 2 (p130) hs
HG-U133A_2:212331_AT retinoblastoma-like 2 (p130) hs
HG-FOCUS:212331_AT retinoblastoma-like 2 (p130) hs
HG-U133_PLUS_2:212331_AT retinoblastoma-like 2 (p130) hs
HG-U133A:212331_AT retinoblastoma-like 2 (p130) hs
HG-U133A_2:212332_AT retinoblastoma-like 2 (p130) hs
HG-U133_PLUS_2:212332_AT retinoblastoma-like 2 (p130) hs
HG-U133A:212332_AT retinoblastoma-like 2 (p130) hs
HG-U95AV2:32597_AT retinoblastoma-like 2 (p130) hs
HG-U95D:79041_AT retinoblastoma-like 2 (p130) hs
HG-U95AV2:32596_AT Retinoblastoma-like 2 (p130) hs
MOUSE430_2:1425981_A_AT retinoblastoma-like 2 mm
MOE430A:1425981_A_AT retinoblastoma-like 2 mm
MOUSE430A_2:1425981_A_AT retinoblastoma-like 2 mm
MU11KSUBA:U36799_S_AT retinoblastoma-like 2 mm
MG-U74AV2:95617_AT retinoblastoma-like 2 mm
MOE430A:1418146_A_AT retinoblastoma-like 2 mm
MOUSE430A_2:1418146_A_AT retinoblastoma-like 2 mm
MOUSE430_2:1418146_A_AT retinoblastoma-like 2 mm
MU19KSUBB:TC30332_AT Retinoblastoma-like 2, mRNA (cDNA clone MGC:18400 IMAGE:4240592) mm
MU19KSUBB:TC30333_S_AT Retinoblastoma-like 2, mRNA (cDNA clone MGC:18400 IMAGE:4240592) mm
MU19KSUBB:TC32860_AT Retinoblastoma-like 2, mRNA (cDNA clone MGC:18400 IMAGE:4240592) mm
MU19KSUBB:TC32860_G_AT Retinoblastoma-like 2, mRNA (cDNA clone MGC:18400 IMAGE:4240592) mm
RG-U34A:RC_AI227715_AT retinoblastoma-like 2 rn
RAE230A:1368403_AT retinoblastoma-like 2 rn
RAT230_2:1368403_AT retinoblastoma-like 2 rn
RAE230B:1378190_AT Retinoblastoma-like 2 rn
RAT230_2:1378190_AT Retinoblastoma-like 2 rn
RAT230_2:1395140_AT Retinoblastoma-like 2 rn
RAE230B:1395140_AT Retinoblastoma-like 2 rn
RG-U34B:RC_AA945871_AT Retinoblastoma-like 2 rn
Protein Similarities
Method ID Description E-Value
blast AAC24266 Abnormal cell lineage protein 35 [Caenorhabditis elegans] ref|NP_491686.1| abnormal cell LINeage family member (lin-35) [Caenorhabditis elegans] gb|AAD05570.1| synthetic multivulva protein LIN-35 Rb [Caenorhabditis elegans] 0.0
blast CAE60456 Hypothetical protein CBG04064 [Caenorhabditis briggsae] 0.0
blast EAL31534 GA20332-PA [Drosophila pseudoobscura] 2.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR002720 EMBL-EBI Retinoblastoma-associated protein, A-box 1.7E-96
Pfam IPR002719 EMBL-EBI Retinoblastoma-associated protein, B-box 1.4E-90
Pfam IPR002720 EMBL-EBI Retinoblastoma-associated protein, A-box 1.7E-96
Pfam IPR002719 EMBL-EBI Retinoblastoma-associated protein, B-box 1.4E-90
Pfam IPR002720 EMBL-EBI Retinoblastoma-associated protein, A-box 1.7E-96
Pfam IPR002719 EMBL-EBI Retinoblastoma-associated protein, B-box 1.4E-90
Sequence
Target Sequence 
>C. ELEGANS:188392_S_AT
gatcgtgtttcaatgggagctcgtgcaaaatcacaatgctggtcacttttcgattatctt
cttcgcaatgacactttgatttttatggatagacatcttgatcaaattcttctttgttgc
gtgtttgtcattatgaagataaatgagtcatcaatgcttttcacggaaataatggctcaa
tatcgacgacaatcagccaattctttgctggtctaccgaagtgttacagtattccaagaa
caactgaatcccgaaaatccacaggcagtaaacacgaaggagacaattttggaacgtctt
gaaggtccacaaaaagaaaaaacgacagttgatataatcaaatattataatatcgagttt
cgggatcgtatcaagtatattatcggtcaaattgatagtgcttcagatgaagatttgatg
gaaatgccggttgcaacagaatctggattgatgcctgttcgagtttatttaacacataaa
ttatcgattcaaacgcttccaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCGTGTTTCAATGGGAGCTCGTG 210 417 2305 Antisense
TGCTGGTCACTTTTCGATTATCTTC 230 583 2341 Antisense
GATTATCTTCTTCGCAATGACACTT 2 431 2356 Antisense
AATTCTTCTTTGTTGCGTGTTTGTC 39 177 2409 Antisense
GAGTCATCAATGCTTTTCACGGAAA 26 401 2449 Antisense
ATCGACGACAATCAGCCAATTCTTT 660 35 2486 Antisense
CCAATTCTTTGCTGGTCTACCGAAG 612 275 2501 Antisense
GAATCCCGAAAATCCACAGGCAGTA 470 329 2550 Antisense
ATGCCGGTTGCAACAGAATCTGGAT 77 41 2728 Antisense
ATCTGGATTGATGCCTGTTCGAGTT 16 35 2745 Antisense
AATTATCGATTCAAACGCTTCCAAA 440 175 2783 Antisense
Sequence Source Affymetrix Proprietary Database