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[188391_at]

Description F59E12.12 /REP_DB=WormBase Gene ID /WP=CE11540 /GEN=bli-2 /TR=O01904 /GB=AAB54250.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 58      6grp_cgc > 88      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 11.7
(-2.9)
A 		71.8
(3)
A 		61.7
(2)
A 		10.7
(-3)
A 		61
B 46.3
(3)
A 		21.4
(-0.7)
A 		29.6
(0.5)
A 		6
(-3)
A 		40
C 19.5
(-2)
A 		23.7
(-1.5)
A 		57.3
(3)
A 		12.1
(-3)
A 		45
avr 25.8
(-0.6) 		39
(1.4) 		49.5
(3) 		9.6
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1072
1 2 3 4 5 6 range
A 7.4
(-3)
A 		63.3
(-2.6)
A 		16.4
(-2.9)
A 		671.7
(1.3)
P 		943.8
(3)
P 		305.7
(-1.1)
P 		936
B 20.4
(-3)
A 		41
(-2.9)
A 		46.8
(-2.8)
A 		535
(0.2)
P 		983.5
(3)
P 		330
(-1.1)
P 		963
C 5.8
(-3)
A 		52.7
(-2.7)
A 		49.4
(-2.8)
A 		503
(-0.2)
P 		1077.3
(3)
P 		166.7
(-2.1)
P 		1072
avr 11.2
(-3) 		52.3
(-2.8) 		37.5
(-2.8) 		569.9
(0.4) 		1001.5
(3) 		267.5
(-1.4) 		990
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 4.3
(-1.9)
A 		15.6
(2.8)
A 		16.1
(3)
A 		1.6
(-3)
A 		15
B 14.7
(3)
A 		5.3
(-1.2)
A 		6.9
(-0.5)
A 		1.3
(-3)
A 		13
C 6.5
(-1.5)
A 		6.8
(-1.4)
A 		20
(3)
A 		2
(-3)
A 		18
avr 8.5
(0.3) 		9.2
(0.6) 		14.3
(3) 		1.6
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 398
1 2 3 4 5 6 range
A 1.4
(-3)
A 		22.5
(-2.6)
A 		3.6
(-3)
A 		170.3
(0.6)
P 		284.8
(3)
P 		74.4
(-1.5)
P 		283
B 7.1
(-3)
A 		13.7
(-2.9)
A 		11.4
(-2.9)
A 		141.7
(-0.9)
P 		399.6
(3)
P 		123.1
(-1.2)
P 		393
C 2.1
(-3)
A 		16.1
(-2.8)
A 		12.6
(-2.8)
A 		101.1
(-1.3)
P 		348.6
(3)
P 		55.8
(-2.1)
P 		347
avr 3.5
(-3) 		17.4
(-2.8) 		9.2
(-2.9) 		137.7
(-0.6) 		344.3
(3) 		84.4
(-1.6) 		341
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188391_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4632
Sequence Type Exemplar sequence
Representative Public ID F59E12.12 NCBI
Target Description F59E12.12 /REP_DB=WormBase Gene ID /WP=CE11540 /GEN=bli-2 /TR=O01904 /GB=AAB54250.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062703(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062703 NCBI Caenorhabditis elegans BLIstered cuticle family member (bli-2) (bli-2) mRNA, complete cds. 11/11 None
SNAP00000018072 ENSEMBL cdna:SNAP chromosome:CEL140:II:5651408:5652390:-1 11/11 None
GENEFINDER00000018078 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5651408:5652390:-1 11/11 None
F59E12.12 ENSEMBL cdna:known chromosome:CEL140:II:5651379:5652390:-1 gene:F59E12.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5651405-5652388(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol bli-2
Ensembl F59E12.12
Entrez Gene 191611 Entrez gene
SwissProt O01904 EMBL-EBI
WormBase CE11540 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6817 phosphate transport inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
30312 external encapsulating structure inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
42302 structural constituent of cuticle inferred from electronic annotation QuickGO AmiGO
42329 structural constituent of cuticle (sensu Nematoda) inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB54250 Blistered cuticle protein 2 [Caenorhabditis elegans] ref|NP_495104.1| BLIstered cuticle family member (bli-2) [Caenorhabditis elegans] 2.0E-81
blast CAE59157 Hypothetical protein CBG02463 [Caenorhabditis briggsae] 2.0E-76
blast CAE59586 Hypothetical protein CBG02987 [Caenorhabditis briggsae] 1.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR002486 EMBL-EBI Nematode cuticle collagen, N-terminal 2.2E-11
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 4.5E-4
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 8.8E-13
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.6E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495104.1 1 17-39
F59E12.12 1 17-39
SNAP00000018072 1 17-39
GENEFINDER00000018078 1 17-39
Sequence
Target Sequence 
>C. ELEGANS:188391_AT
agtagcagcaaatcctctttggctccaggaggacaatgctgttcttgcaaaaccggacca
tctggaccaccaggaccaccaggagaagatggacgggatggtagagatggaaaaccagga
cttaatggagaagacggtacagatgcaaaggattcagctccacgacgtgatgcagctgca
ccatgttacgattgccctgtagggccaccaggaccaccaggaaatattggatcaaaggga
caaccaggaagaaacgggaaagatggtcttccaggagttccaggactaccaggtcagcca
ggagaaccaggagatgatggagagcctggagaggatggagacccaggtcagccgggagat
aacggagagccaggaaaatgtgatgaagtgaacgttgctcaagggcctccaggaagtccg
ggacctccaggattaccaggacctgacgggctgcctggaactccaggaaatccaggacaa
gacggtgaacaaggaccagcaggtgagcctggaagagacgggaaagatggtcaaccagga
agacccggacagcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTAGCAGCAAATCCTCTTTGGCTC 265 61 319 Antisense
TGCTGTTCTTGCAAAACCGGACCAT 45 583 355 Antisense
AAAGGATTCAGCTCCACGACGTGAT 341 121 465 Antisense
CACGACGTGATGCAGCTGCACCATG 513 199 479 Antisense
AGCTGCACCATGTTACGATTGCCCT 390 81 492 Antisense
GATTGCCCTGTAGGGCCACCAGGAC 319 433 508 Antisense
GAGACCCAGGTCAGCCGGGAGATAA 83 395 656 Antisense
GAAGTGAACGTTGCTCAAGGGCCTC 615 335 703 Antisense
GGATTACCAGGACCTGACGGGCTGC 391 509 748 Antisense
TGACGGGCTGCCTGGAACTCCAGGA 178 571 762 Antisense
TCAACCAGGAAGACCCGGACAGCCA 393 631 849 Antisense
Sequence Source Affymetrix Proprietary Database