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[188386_at] cuticulin

Description W01A8.3 /REP_DB=WormBase Gene ID /WP=CE06533 /TR=Q23097 /GB=CAA95850.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=cuticulin
Cluster 6grp_total > 07      4grp_total > 43      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 30.4
(-1.3)
A 		74.3
(3)
A 		42
(-0.1)
A 		12.4
(-3)
A 		62
B 15.3
(-1.8)
A 		57.8
(3)
A 		11.4
(-2.3)
A 		5.2
(-3)
A 		53
C 29.6
(-1.1)
A 		75.3
(3)
A 		27.6
(-1.3)
A 		8.2
(-3)
A 		67
avr 25.1
(-1.4) 		69.1
(3) 		27
(-1.2) 		8.6
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 93.1
(3)
P 		9.2
(-3)
A 		25.1
(-1.9)
A 		10.6
(-2.9)
A 		35.4
(-1.1)
A 		22.4
(-2.1)
A 		84
B 57
(1.7)
P 		66.7
(3)
M 		55.1
(1.4)
A 		46.3
(0.2)
A 		22.9
(-3)
A 		34.1
(-1.5)
A 		44
C 52.2
(1.6)
P 		57.5
(2.2)
P 		38.3
(-0)
A 		64.4
(3)
A 		12.5
(-3)
A 		45.9
(0.9)
A 		52
avr 67.4
(3) 		44.5
(-0.1) 		39.5
(-0.8) 		40.4
(-0.7) 		23.6
(-3) 		34.1
(-1.6) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 11.1
(0.9)
A 		16.1
(3)
A 		11
(0.9)
A 		1.8
(-3)
A 		14
B 4.9
(-1.3)
A 		14.3
(3)
A 		2.6
(-2.3)
A 		1.1
(-3)
A 		13
C 9.8
(-0.5)
A 		21.7
(3)
A 		9.6
(-0.6)
A 		1.4
(-3)
A 		20
avr 8.6
(-0.3) 		17.4
(3) 		7.7
(-0.6) 		1.4
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 17.2
(3)
P 		3.3
(-2.8)
A 		5.5
(-1.8)
A 		2.7
(-3)
A 		10.7
(0.3)
A 		5.4
(-1.9)
A 		15
B 19.8
(1.9)
P 		22.2
(3)
M 		13.4
(-1.1)
A 		12.3
(-1.6)
A 		9.3
(-3)
A 		12.7
(-1.4)
A 		13
C 19.4
(3)
P 		17.5
(2.3)
P 		9.8
(-0.8)
A 		13
(0.5)
A 		4.1
(-3)
A 		15.4
(1.4)
A 		15
avr 18.8
(3) 		14.3
(0.5) 		9.6
(-2.1) 		9.3
(-2.3) 		8
(-3) 		11.2
(-1.2) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188386_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.343
Sequence Type Exemplar sequence
Representative Public ID W01A8.3 NCBI
Target Description W01A8.3 /REP_DB=WormBase Gene ID /WP=CE06533 /TR=Q23097 /GB=CAA95850.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=cuticulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059599(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059599 NCBI Caenorhabditis elegans W01A8.3 (cuticulin) mRNA, complete cds. 11/11 None
GENEFINDER00000018616 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7073395:7076031:1 11/11 None
SNAP00000018610 ENSEMBL cdna:SNAP chromosome:CEL140:I:7074286:7076031:1 11/11 None
W01A8.3 ENSEMBL cdna:known chromosome:CEL140:I:7074374:7076031:1 gene:W01A8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7073780-7075962(+) 100.0 100.0
Public Domain and Genome References
Gene Title cuticulin
Ensembl W01A8.3
Entrez Gene 189075 Entrez gene
SwissProt Q23097 EMBL-EBI
WormBase CE28693 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA95850 Hypothetical protein W01A8.3 [Caenorhabditis elegans] ref|NP_492000.1| W01A8.3 [Caenorhabditis elegans] 0.0
blast CAE65707 Hypothetical protein CBG10782 [Caenorhabditis briggsae] 0.0
blast CAE65707 Hypothetical protein CBG10782 [Caenorhabditis briggsae] 1.0E-174
blast CAE60481 Hypothetical protein CBG04095 [Caenorhabditis briggsae] 7.0E-62
blast CAE60481 Hypothetical protein CBG04095 [Caenorhabditis briggsae] 1.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 1.0E-65
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 1.0E-65
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 1.0E-65
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492000.1 1 309-331
W01A8.3 1 309-331
GENEFINDER00000018616 1 334-356
SNAP00000018610 1 341-363
Sequence
Target Sequence 
>C. ELEGANS:188386_AT
gtatgggaatgtacaccggctgatatgggaatgcttgtcaaaaagtgttttgtaacagat
ggagacggagaagatcatgctgttgttgactttgatggctgtgcaactgatccattcttg
ctcagtgagctgtcttacgatgcttcacttatgcgagctcatgcatcaagtcaagttttc
aaatatgcagactcaaatcaattatatttcacttgtcagataagattgtgtcagaaacaa
atgggaatgtgccaggaagttacgccaccaaactgtggagtcaagaaattgctgagtgaa
gcaagtggtgatggaaatcgaacaaaaagagaagctgatcggtctgattatgagatcgac
gtagcaacatcagaacttcttgtacttgatccagcagatcgtgggctccttgctccttct
ccattctgtgttccacgactacttcttccagttcttccccttattcttatcacaattgta
tcattaacagttgtctccacagcactagtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATGGGAATGTACACCGGCTGATA 110 457 607 Antisense
TGTTGTTGACTTTGATGGCTGTGCA 336 553 687 Antisense
GTGCAACTGATCCATTCTTGCTCAG 512 481 707 Antisense
GTGAGCTGTCTTACGATGCTTCACT 142 483 731 Antisense
GATGCTTCACTTATGCGAGCTCATG 346 413 745 Antisense
GCGAGCTCATGCATCAAGTCAAGTT 113 291 759 Antisense
ATGTGCCAGGAAGTTACGCCACCAA 144 47 853 Antisense
CGACGTAGCAACATCAGAACTTCTT 142 251 963 Antisense
GAACTTCTTGTACTTGATCCAGCAG 643 349 979 Antisense
TCCATTCTGTGTTCCACGACTACTT 166 591 1026 Antisense
AACAGTTGTCTCCACAGCACTAGTG 135 137 1092 Antisense
Sequence Source Affymetrix Proprietary Database