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[188383_at] helicase

Description T23H2.3 /REP_DB=WormBase Gene ID /WP=CE14110 /TR=P91494 /GB=AAC48202.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=helicase
Cluster 6grp_high1_10_10 > 99      4grp_high_8_8 > 63      6grp_total > 99      4grp_total > 48      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 76.3
(-2)
P 		87.3
(0.6)
P 		97.6
(3)
P 		72.2
(-3)
P 		25
B 48.6
(-3)
A 		84
(-0.5)
P 		135
(3)
P 		70.3
(-1.5)
P 		86
C 61.5
(-3)
P 		85.5
(-0.5)
P 		119.2
(3)
P 		76.7
(-1.4)
A 		58
avr 62.1
(-3) 		85.6
(-0.4) 		117.3
(3) 		73.1
(-1.8) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 58.8
(-3)
A 		76
(-2.2)
P 		111.4
(-0.5)
P 		92.1
(-1.4)
P 		178.8
(2.8)
P 		183.1
(3)
P 		124
B 68.6
(-3)
P 		100.7
(-1)
P 		94.5
(-1.4)
P 		91.5
(-1.6)
P 		164.7
(3)
P 		119.4
(0.2)
P 		96
C 97.5
(-1.2)
P 		102.2
(-0.8)
P 		116.2
(0.5)
P 		77.9
(-3)
P 		126.2
(1.4)
P 		143.9
(3)
P 		66
avr 75
(-3) 		93
(-1.7) 		107.4
(-0.6) 		87.2
(-2.1) 		156.6
(3) 		148.8
(2.4) 		82
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 27.8
(3)
P 		19
(-0.1)
P 		25.5
(2.2)
P 		10.6
(-3)
P 		17
B 15.5
(-2.8)
A 		20.7
(-0.9)
P 		31.3
(3)
P 		15
(-3)
P 		16
C 20.4
(-1.4)
P 		24.6
(-0.6)
P 		41.6
(3)
P 		12.9
(-3)
A 		29
avr 21.2
(-0.5) 		21.4
(-0.4) 		32.8
(3) 		12.8
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 10.8
(-3)
A 		27
(-0.8)
P 		24.3
(-1.1)
P 		23.4
(-1.3)
P 		54
(3)
P 		44.6
(1.7)
P 		43
B 23.9
(-2.9)
P 		33.5
(-1.6)
P 		23
(-3)
P 		24.2
(-2.8)
P 		66.9
(3)
P 		44.5
(-0.1)
P 		44
C 36.1
(0.8)
P 		31.2
(-0.1)
P 		29.6
(-0.4)
P 		15.7
(-3)
P 		40.8
(1.6)
P 		48.2
(3)
P 		33
avr 23.6
(-2.5) 		30.6
(-1.3) 		25.6
(-2.2) 		21.1
(-3) 		53.9
(3) 		45.8
(1.5) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188383_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2334
Sequence Type Exemplar sequence
Representative Public ID T23H2.3 NCBI
Target Description T23H2.3 /REP_DB=WormBase Gene ID /WP=CE14110 /TR=P91494 /GB=AAC48202.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059453(11), NM_182073(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059453 NCBI Caenorhabditis elegans T23H2.3a (helicase) mRNA, complete cds. 11/11 None
NM_182073 NCBI Caenorhabditis elegans T23H2.3b (helicase) mRNA, complete cds. 11/11 None
SNAP00000032984 ENSEMBL cdna:SNAP chromosome:CEL140:I:6450002:6454599:-1 11/11 None
GENEFINDER00000032990 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6450002:6454599:-1 11/11 None
T23H2.3a ENSEMBL cdna:known chromosome:CEL140:I:6449789:6454599:-1 gene:T23H2.3 11/11 None
T23H2.3b ENSEMBL cdna:known chromosome:CEL140:I:6450002:6453377:-1 gene:T23H2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6449930-6454528(-) 100.0 100.0
Public Domain and Genome References
Gene Title helicase
Ensembl T23H2.3
Entrez Gene 172347 Entrez gene
SwissProt P91494 EMBL-EBI
WormBase CE30397 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAM15608 Hypothetical protein T23H2.3 [Caenorhabditis elegans] ref|NP_491854.2| T23H2.3a [Caenorhabditis elegans] 0.0
blast T28968 hypothetical protein T23H2.3 - Caenorhabditis elegans 0.0
blast NP_871873 T23H2.3b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000330 EMBL-EBI SNF2 related domain 1.1E-62
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 7.1E-20
Pfam IPR000330 EMBL-EBI SNF2 related domain 1.1E-62
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 7.1E-20
Sequence
Target Sequence 
>C. ELEGANS:188383_AT
ttgagcccgatttcattagctgcaagatggccaaaacgctggaagttgttcgtgaaattt
tggagaagaaggagaaagttgtgattgtctcccagtggacttctgtgctgaatcttgtgg
aaaagcatattcaagcaggcggccacaactatacatcaatcaccggccaggttcttgtca
aagatcgccaagaacgagttgacagtttcaaccaggaaaaaggcggagctcaggtgatgc
tgctctcattgactgccggaggtgttggactcaatttgattggaggaaatcatctgatta
tggttgatttgcactggaatcccgcactggaacagcaggcttgtgacagaatttatcgaa
tgggacagaagaaggaagtctttattcatagactaaatatttcaaaattttgcagattaa
tcgtgaaggggaccatcgagcaacgagtcatggatctccaggagaagaagctcgcattgg
cagcttcggtgttgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGAGCCCGATTTCATTAGCTGCAA 13 707 2510 Antisense
TTGTGATTGTCTCCCAGTGGACTTC 146 709 2588 Antisense
GTGGACTTCTGTGCTGAATCTTGTG 155 491 2604 Antisense
GCATATTCAAGCAGGCGGCCACAAC 703 303 2634 Antisense
CACAACTATACATCAATCACCGGCC 22 195 2653 Antisense
TCACCGGCCAGGTTCTTGTCAAAGA 395 625 2669 Antisense
TCAGGTGATGCTGCTCTCATTGACT 413 623 2739 Antisense
GACTGCCGGAGGTGTTGGACTCAAT 473 373 2760 Antisense
AATCCCGCACTGGAACAGCAGGCTT 86 169 2827 Antisense
GAGCAACGAGTCATGGATCTCCAGG 311 391 2947 Antisense
TCGCATTGGCAGCTTCGGTGTTGGA 370 599 2981 Antisense
Sequence Source Affymetrix Proprietary Database