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[188377_at] protease inhibitor

Description T21D12.7 /REP_DB=WormBase Gene ID /WP=CE26005 /GB=AAK72062.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protease inhibitor
Cluster 6grp_high1_10_10 > 90      6grp_total > 82      4grp_total > 49      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 335
1 2 3 4 range
A 76.8
(-3)
M 		354
(2.2)
P 		398.6
(3)
P 		77.7
(-3)
A 		322
B 65.6
(-3)
P 		385.9
(2.7)
P 		400.2
(3)
P 		70.9
(-2.9)
A 		335
C 74
(-3)
P 		396.9
(3)
P 		334.8
(1.8)
P 		110
(-2.3)
A 		323
avr 72.1
(-3) 		378.9
(3) 		377.9
(3) 		86.2
(-2.7) 		307

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 198
1 2 3 4 5 6 range
A 118
(-3)
M 		218
(0.2)
P 		191.5
(-0.7)
M 		305.7
(3)
P 		300.7
(2.8)
P 		175.1
(-1.2)
P 		188
B 108.2
(-3)
P 		253.8
(3)
P 		174.4
(-0.3)
P 		176.7
(-0.2)
P 		197.3
(0.7)
P 		173.7
(-0.3)
P 		146
C 120.4
(-3)
P 		294.9
(3)
P 		214.7
(0.2)
A 		211.5
(0.1)
P 		290.6
(2.9)
P 		131.5
(-2.6)
P 		175
avr 115.5
(-3) 		255.6
(2.7) 		193.5
(0.2) 		231.3
(1.7) 		262.9
(3) 		160.1
(-1.2) 		147
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 28
(-1.9)
M 		76.8
(1.2)
P 		104.2
(3)
P 		11.4
(-3)
A 		93
B 20.9
(-2.6)
P 		95.3
(3)
P 		92.7
(2.8)
P 		15.1
(-3)
A 		80
C 24.5
(-2.6)
P 		114.1
(2.8)
P 		116.7
(3)
P 		18.5
(-3)
A 		98
avr 24.5
(-2.4) 		95.4
(2.4) 		104.5
(3) 		15
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 21.7
(-3)
M 		77.5
(1.9)
P 		41.7
(-1.3)
M 		77.5
(1.9)
P 		90.7
(3)
P 		42.6
(-1.2)
P 		69
B 37.6
(-3)
P 		84.5
(3)
P 		42.3
(-2.4)
P 		46.8
(-1.8)
P 		80.2
(2.4)
P 		64.8
(0.5)
P 		47
C 44.6
(-2.8)
P 		90
(2.5)
P 		54.6
(-1.6)
A 		42.5
(-3)
P 		94
(3)
P 		44
(-2.8)
P 		52
avr 34.6
(-3) 		84
(2.5) 		46.2
(-1.7) 		55.6
(-0.7) 		88.3
(3) 		50.5
(-1.2) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188377_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10598
Sequence Type Exemplar sequence
Representative Public ID T21D12.7 NCBI
Target Description T21D12.7 /REP_DB=WormBase Gene ID /WP=CE26005 /GB=AAK72062.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protease inhibitor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067498(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067498 NCBI Caenorhabditis elegans T21D12.7 (T21D12.7) mRNA, complete cds. 11/11 None
T21D12.7 ENSEMBL cdna:known chromosome:CEL140:IV:287344:291352:-1 gene:T21D12.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000015224 5/11 Cross Hyb Matching Probes None
SNAP00000015225 6/11 Cross Hyb Matching Probes None
GENEFINDER00000015233 5/11 Cross Hyb Matching Probes None
GENEFINDER00000015235 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:287410-291352(-) 100.0 100.0
Public Domain and Genome References
Gene Title protease inhibitor
Ensembl T21D12.7
Entrez Gene 176850 Entrez gene
SwissProt Q965M1 EMBL-EBI
WormBase CE31623 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
16042 lipid catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4623 phospholipase A2 activity inferred from electronic annotation QuickGO AmiGO
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK72062 Hypothetical protein T21D12.7 [Caenorhabditis elegans] ref|NP_499899.2| T21D12.7 [Caenorhabditis elegans] 0.0
blast T32980 hypothetical protein T21D12.7 - Caenorhabditis elegans 0.0
blast CAE67905 Hypothetical protein CBG13502 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 4.3E-20
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 2.3E-22
Sequence
Target Sequence 
>C. ELEGANS:188377_AT
gtttttctgttgtcacactgtttcaatattcagctgtcctgatggtgctagtccattctt
ggatccaaattcaggccaacctgccacgtgtctggcttcgaatccattttcctgtccagc
agagcacagttgcactgcattgatggatggttcaactgcttgctgtccaatccaaacacc
tctctgtgtagaagcccttgtatcagatgatgggtccccgaagacatgtctaggatggga
tgataatacaacgtgtccccagggaaaatgccagaaagctatggacggtattttacaaaa
aatgcatcattcaatatcagttatcctggcactagtgatatcaatatcacatgctttatt
gacgtcggacccatccaattttgcaattcgtcaatttcctgaaccaatgtcaatgtcatc
cgaatgtaatacttttgatgaagctcacttttgtaaaacgataacattagactccgctct
gaacgccctggcagaatcccacaagtgcgagtgtccagtaaactttcggtgtccagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTTTCTGTTGTCACACTGTTTCA 474 449 1458 Antisense
TCAGCTGTCCTGATGGTGCTAGTCC 63 625 1487 Antisense
GGTGCTAGTCCATTCTTGGATCCAA 394 501 1501 Antisense
ATGGTTCAACTGCTTGCTGTCCAAT 608 51 1604 Antisense
AACACCTCTCTGTGTAGAAGCCCTT 80 135 1632 Antisense
TGATGGGTCCCCGAAGACATGTCTA 319 567 1665 Antisense
CACATGCTTTATTGACGTCGGACCC 639 195 1805 Antisense
GTCGGACCCATCCAATTTTGCAATT 139 473 1821 Antisense
TAACATTAGACTCCGCTCTGAACGC 434 639 1919 Antisense
GAATCCCACAAGTGCGAGTGTCCAG 438 329 1951 Antisense
GTCCAGTAAACTTTCGGTGTCCAGC 251 473 1970 Antisense
Sequence Source Affymetrix Proprietary Database