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[188358_at]

Description B0218.1 /REP_DB=WormBase Gene ID /WP=CE06684 /TR=Q17449 /GB=AAB00667.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=amidase
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 01      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 23      6grp_total > 23      4grp_total > 46      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2284
1 2 3 4 range
A 3449.3
(3)
P 		1609.3
(-1.9)
P 		1195.8
(-3)
P 		1177.6
(-3)
P 		2272
B 3004.5
(3)
P 		1433.3
(-2.1)
P 		1165.4
(-3)
P 		1335.5
(-2.4)
P 		1839
C 3292.8
(3)
P 		1410.7
(-2.7)
P 		1486.2
(-2.4)
P 		1298.5
(-3)
P 		1994
avr 3248.9
(3) 		1484.4
(-2.4) 		1282.5
(-3) 		1270.5
(-3) 		1978

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1651
1 2 3 4 5 6 range
A 2707.7
(2.8)
P 		2740.3
(3)
P 		2178.2
(0.3)
P 		2708.2
(2.8)
P 		1470.2
(-3)
P 		1601.7
(-2.4)
P 		1270
B 2665.2
(3)
P 		2197.4
(0.6)
P 		2416.9
(1.7)
P 		1993.8
(-0.4)
P 		1507.7
(-2.9)
P 		1494.4
(-3)
P 		1171
C 3121
(3)
P 		1966.2
(-1.6)
P 		2755.1
(1.5)
P 		2286.9
(-0.3)
P 		1607
(-3)
P 		1639.8
(-2.9)
P 		1514
avr 2831.3
(3) 		2301.3
(0.6) 		2450.1
(1.2) 		2329.6
(0.7) 		1528.3
(-3) 		1578.6
(-2.8) 		1303
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1084
1 2 3 4 range
A 1255.9
(3)
P 		349.3
(-2)
P 		312.6
(-2.2)
P 		172.4
(-3)
P 		1084
B 958.1
(3)
P 		354
(-2.3)
P 		270.1
(-3)
P 		284.6
(-2.9)
P 		688
C 1090
(3)
P 		405.6
(-1.7)
P 		518
(-0.9)
P 		218.9
(-3)
P 		871
avr 1101.3
(3) 		369.6
(-2) 		366.9
(-2) 		225.3
(-3) 		876

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 767
1 2 3 4 5 6 range
A 499.1
(-1.9)
P 		974.6
(3)
P 		474.4
(-2.1)
P 		686.9
(0)
P 		443.7
(-2.4)
P 		390
(-3)
P 		585
B 927.4
(3)
P 		731.4
(0.1)
P 		586.9
(-2.1)
P 		528.1
(-3)
P 		612.6
(-1.7)
P 		557.2
(-2.6)
P 		399
C 1156.8
(3)
P 		599.9
(-1.8)
P 		701.2
(-0.9)
P 		459.9
(-3)
P 		520
(-2.5)
P 		548.9
(-2.2)
P 		697
avr 861.1
(3) 		768.6
(1.5) 		587.5
(-1.5) 		558.3
(-2) 		525.4
(-2.6) 		498.7
(-3) 		362
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188358_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9585
Sequence Type Exemplar sequence
Representative Public ID B0218.1 NCBI
Target Description B0218.1 /REP_DB=WormBase Gene ID /WP=CE06684 /TR=Q17449 /GB=AAB00667.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=amidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068967(11), NM_182295(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068967 NCBI Caenorhabditis elegans B0218.1a (amidase) mRNA, complete cds. 11/11 None
NM_182295 NCBI Caenorhabditis elegans B0218.1b (amidase) mRNA, complete cds. 11/11 None
SNAP00000005555 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8159403:8161922:1 11/11 None
GENEFINDER00000005560 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8159403:8161922:1 11/11 None
B0218.1a.2 ENSEMBL cdna:known chromosome:CEL140:IV:8159339:8162023:1 gene:B0218.1 11/11 None
B0218.1a.1 ENSEMBL cdna:known chromosome:CEL140:IV:8159368:8162023:1 gene:B0218.1 11/11 None
B0218.1a.5 ENSEMBL cdna:known chromosome:CEL140:IV:8159388:8161927:1 gene:B0218.1 11/11 None
B0218.1a.3 ENSEMBL cdna:known chromosome:CEL140:IV:8159389:8161986:1 gene:B0218.1 11/11 None
B0218.1a.4 ENSEMBL cdna:known chromosome:CEL140:IV:8159397:8161986:1 gene:B0218.1 11/11 None
B0218.1b ENSEMBL cdna:known chromosome:CEL140:IV:8159403:8161922:1 gene:B0218.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8159408-8161928(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0218.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB00667 Hypothetical protein B0218.1a [Caenorhabditis elegans] ref|NP_501368.1| B0218.1a [Caenorhabditis elegans] 0.0
blast CAE58426 Hypothetical protein CBG01557 [Caenorhabditis briggsae] 0.0
blast AAO38681 Hypothetical protein B0218.1b [Caenorhabditis elegans] ref|NP_872095.1| B0218.1b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000120 EMBL-EBI Amidase 1.0E-126
Pfam IPR000120 EMBL-EBI Amidase 3.5E-28
Pfam IPR000120 EMBL-EBI Amidase 2.6E-94
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501368.1 1 2-19
NP_872095.1 1 2-19
B0218.1a.2 1 2-19
B0218.1a.1 1 2-19
B0218.1a.5 1 2-19
B0218.1a.3 1 2-19
B0218.1a.4 1 2-19
B0218.1b 1 2-19
SNAP00000005555 1 2-19
GENEFINDER00000005560 1 2-19
Sequence
Target Sequence 
>C. ELEGANS:188358_AT
acgtattcttagctatccagtttcgttggtattcccgagaatgggaatgttgatgcaatc
attgacaagagacacatttgaactccgtgaagcatatgctgatattgaagcctatcgaga
ggaatttgttggtcttatgatgaaagataatttggatgtaatcttatgtccagcttccat
aatgccagctccacaacacgatattccatcaaaggttgtttctggtgtcagttacacttg
tctctacaacttgttagattttggagcaggtgttgttccagtcactgctgtatctaaatc
tgacgaggagaagttgatcaacgaatatccagaaactgataaatggtatcaaatcactaa
aaaagcaacacttggagccgtcggaatgccaattggagttcaagttgctgcaccaccata
ccgagaggaagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGTATTCTTAGCTATCCAGTTTCG 525 91 1251 Antisense
TCCAGTTTCGTTGGTATTCCCGAGA 2 591 1266 Antisense
TTGAACTCCGTGAAGCATATGCTGA 701 701 1328 Antisense
GATGTAATCTTATGTCCAGCTTCCA 367 409 1405 Antisense
TGTCCAGCTTCCATAATGCCAGCTC 154 553 1417 Antisense
GCTCCACAACACGATATTCCATCAA 697 297 1438 Antisense
GTGTCAGTTACACTTGTCTCTACAA 583 485 1475 Antisense
CAGTCACTGCTGTATCTAAATCTGA 365 207 1529 Antisense
GAGCCGTCGGAATGCCAATTGGAGT 218 385 1625 Antisense
TGGAGTTCAAGTTGCTGCACCACCA 566 549 1644 Antisense
CTGCACCACCATACCGAGAGGAAGC 505 237 1658 Antisense
Sequence Source Affymetrix Proprietary Database