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[188353_s_at]

Description C05E11.8 /REP_DB=WormBase Gene ID /WP=CE03938 /GEN=flp-12 /TR=Q17668 /GB=AAA96196.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 40      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 36      6grp_total > 40      4grp_total > 12      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8071
1 2 3 4 range
A 3944.6
(-0.7)
P 		1447
(-2.8)
P 		1199.7
(-3)
P 		8507.7
(3)
P 		7308
B 3194.9
(-1.5)
P 		1834
(-2.5)
P 		1179.4
(-3)
P 		9250.1
(3)
P 		8071
C 3922.7
(-0.9)
P 		1877.3
(-2.6)
P 		1450.7
(-3)
P 		8460.8
(3)
P 		7010
avr 3687.4
(-1.1) 		1719.4
(-2.6) 		1276.6
(-3) 		8739.5
(3) 		7463

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2546
1 2 3 4 5 6 range
A 3119.7
(-0.7)
P 		3439.5
(0.3)
P 		4243.7
(3)
P 		3537.5
(0.7)
P 		2578.7
(-2.5)
P 		2429.8
(-3)
P 		1814
B 4116
(0.3)
P 		4012.9
(0)
P 		4975.5
(3)
P 		4001.6
(-0)
P 		3031.4
(-3)
P 		3037.2
(-3)
P 		1944
C 3657.5
(-0.2)
P 		3815.5
(0.3)
P 		3751.2
(0.1)
P 		4615.4
(3)
P 		2809.6
(-3)
P 		3540.5
(-0.6)
P 		1806
avr 3631.1
(0.3) 		3756
(0.8) 		4323.5
(3) 		4051.5
(1.9) 		2806.6
(-3) 		3002.5
(-2.2) 		1517
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1698
1 2 3 4 range
A 1436.2
(3)
P 		314.1
(-3)
P 		313.6
(-3)
P 		1245.4
(2)
P 		1123
B 1018.8
(-0.4)
P 		453
(-2.4)
P 		273.3
(-3)
P 		1971.2
(3)
P 		1698
C 1298.5
(2.2)
P 		539.7
(-2.8)
P 		505.6
(-3)
P 		1426.6
(3)
P 		921
avr 1251.2
(1.5) 		435.6
(-2.6) 		364.2
(-3) 		1547.7
(3) 		1184

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 857
1 2 3 4 5 6 range
A 575.1
(-3)
P 		1223.3
(3)
P 		924.2
(0.2)
P 		897.2
(-0)
P 		778.2
(-1.1)
P 		591.6
(-2.8)
P 		648
B 1432.2
(3)
P 		1335.7
(1.4)
P 		1208.2
(-0.6)
P 		1059.9
(-3)
P 		1231.6
(-0.2)
P 		1132.5
(-1.8)
P 		372
C 1355.7
(3)
P 		1164.2
(0.4)
P 		954.7
(-2.4)
P 		928.2
(-2.7)
P 		909.1
(-3)
P 		1185.2
(0.7)
P 		447
avr 1121
(0.4) 		1241.1
(3) 		1029
(-1.6) 		961.8
(-3) 		973
(-2.8) 		969.8
(-2.8) 		279
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188353_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16501
Sequence Type Exemplar sequence
Representative Public ID C05E11.8 NCBI
Target Description C05E11.8 /REP_DB=WormBase Gene ID /WP=CE03938 /GEN=flp-12 /TR=Q17668 /GB=AAA96196.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076388(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076388 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-12) (flp-12) mRNA, complete cds. 11/11 None
SNAP00000025332 ENSEMBL cdna:SNAP chromosome:CEL140:X:4591399:4592297:-1 11/11 None
C05E11.8 ENSEMBL cdna:known chromosome:CEL140:X:4591251:4592335:-1 gene:C05E11.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4591399-4592298(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol flp-12
Ensembl C05E11.8
Entrez Gene 180732 Entrez gene
SwissProt Q17668 EMBL-EBI
WormBase CE03938 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA96196 Fmrf-like peptide protein 12 [Caenorhabditis elegans] ref|NP_508789.1| FMRF-Like Peptide family member (flp-12) [Caenorhabditis elegans] gb|AAC08950.1| FMRFamide-like peptide 12 [Caenorhabditis elegans] 1.0E-40
blast CAE68619 Hypothetical protein CBG14501 [Caenorhabditis briggsae] 9.0E-33
blast AAQ23189 FMRFamide-related peptide FLP-12 precursor [Ascaris suum] 1.0E-9
Sequence
Target Sequence 
>C. ELEGANS:188353_S_AT
tgaatgttcaagtcatcatcgcccttttgttctgccttattgcaacatgtgctacacaga
aagtcaaaggatcgcctgaagtactcccggcagccatgtacgatggggaactgagccacg
aaagcgtaaacaagattagtgctcagctgctgaatgctctatcagagttggaagcacttc
aggagggcaaccagcagctcaagatggccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAATGTTCAAGTCATCATCGCCCT 578 575 14 Antisense
CCCTTTTGTTCTGCCTTATTGCAAC 352 257 35 Antisense
GCCTTATTGCAACATGTGCTACACA 688 281 47 Antisense
CAAAGGATCGCCTGAAGTACTCCCG 604 191 78 Antisense
TCCCGGCAGCCATGTACGATGGGGA 3 597 98 Antisense
GGGGAACTGAGCCACGAAAGCGTAA 601 497 118 Antisense
AAACAAGATTAGTGCTCAGCTGCTG 562 129 141 Antisense
CAGCTGCTGAATGCTCTATCAGAGT 672 201 157 Antisense
GCTCTATCAGAGTTGGAAGCACTTC 77 299 169 Antisense
GAAGCACTTCAGGAGGGCAACCAGC 592 339 184 Antisense
GGCAACCAGCAGCTCAAGATGGCCG 533 533 199 Antisense
Sequence Source Affymetrix Proprietary Database