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[188343_at] amidase

Description B0218.2 /REP_DB=WormBase Gene ID /WP=CE06685 /TR=Q17447 /GB=AAB00665.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=amidase
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 57      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 84      6grp_total > 58      4grp_total > 02      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4292
1 2 3 4 range
A 784.1
(-2.9)
P 		744
(-3)
P 		1064
(-2.6)
P 		5035.5
(3)
P 		4292
B 832.9
(-3)
P 		893.8
(-2.9)
P 		1039.2
(-2.6)
P 		3815.1
(3)
P 		2982
C 907.4
(-3)
P 		995.4
(-2.8)
P 		1190.9
(-2.5)
P 		4196.7
(3)
P 		3289
avr 841.5
(-3) 		877.7
(-2.9) 		1098
(-2.6) 		4349.1
(3) 		3508

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 760
1 2 3 4 5 6 range
A 744.1
(-0.2)
P 		563.5
(-1.7)
P 		596.8
(-1.4)
P 		470.8
(-2.4)
P 		393.5
(-3)
P 		1153.9
(3)
P 		760
B 751.6
(-0.4)
P 		616.6
(-1.5)
P 		443.6
(-3)
P 		466.8
(-2.8)
P 		460.1
(-2.9)
P 		1142.8
(3)
P 		699
C 826.4
(0.7)
P 		597.7
(-1.4)
P 		472.6
(-2.5)
P 		419.7
(-3)
P 		437.4
(-2.8)
P 		1073.5
(3)
P 		654
avr 774
(-0) 		592.6
(-1.6) 		504.3
(-2.4) 		452.4
(-2.8) 		430.3
(-3) 		1123.4
(3) 		693
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 652
1 2 3 4 range
A 285.5
(-1.7)
P 		161.5
(-3)
P 		278.1
(-1.8)
P 		737.1
(3)
P 		576
B 265.6
(-2.5)
P 		220.8
(-3)
P 		240.8
(-2.8)
P 		813
(3)
P 		592
C 300.4
(-2.8)
P 		286.2
(-3)
P 		415.1
(-1.2)
P 		707.6
(3)
P 		421
avr 283.8
(-2.3) 		222.8
(-3) 		311.3
(-2) 		752.6
(3) 		530

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 342
1 2 3 4 5 6 range
A 137.2
(-2.3)
P 		200.4
(0)
P 		130
(-2.6)
P 		119.4
(-3)
P 		118.7
(-3)
P 		280.9
(3)
P 		162
B 261.5
(-0.1)
P 		205.2
(-1.2)
P 		107.7
(-3)
P 		123.6
(-2.7)
P 		186.9
(-1.5)
P 		426.2
(3)
P 		319
C 306.3
(1.8)
P 		182.4
(-0.9)
P 		120.3
(-2.2)
P 		84.4
(-3)
P 		141.5
(-1.8)
P 		359.4
(3)
P 		275
avr 235
(0.1) 		196
(-0.9) 		119.3
(-2.8) 		109.1
(-3) 		149
(-2) 		355.5
(3) 		246
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188343_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9200
Sequence Type Exemplar sequence
Representative Public ID B0218.2 NCBI
Target Description B0218.2 /REP_DB=WormBase Gene ID /WP=CE06685 /TR=Q17447 /GB=AAB00665.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=amidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068965(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068965 NCBI Caenorhabditis elegans B0218.2 (amidase) mRNA, complete cds. 11/11 None
SNAP00000005553 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8152432:8154638:1 11/11 None
GENEFINDER00000005562 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8152432:8154638:1 10/11 None
B0218.2.2 ENSEMBL cdna:known chromosome:CEL140:IV:8152424:8154827:1 gene:B0218.2 11/11 None
B0218.2.1 ENSEMBL cdna:known chromosome:CEL140:IV:8152426:8154827:1 gene:B0218.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8152437-8154644(+) 100.0 100.0
Public Domain and Genome References
Gene Title amidase
Ensembl B0218.2
Entrez Gene 177612 Entrez gene
SwissProt Q17447 EMBL-EBI
WormBase CE06685 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1602925_AT similar to fatty acid amide hydrolase cfa
CANINE:1598356_AT similar to fatty acid amide hydrolase cfa
CANINE_2:CFA.19134.1.S1_A_AT similar to fatty acid amide hydrolase cfa
CANINE_2:CFA.19134.1.S1_S_AT similar to fatty acid amide hydrolase cfa
CANINE_2:CFA.8142.1.A1_AT similar to fatty acid amide hydrolase cfa
DROSOPHILA_2:1623486_AT dm
DROSGENOME1:149474_AT dm
CHICKEN:GGA.7143.1.S1_AT similar to fatty acid amide hydrolase gga
CHICKEN:GGA.7143.2.S1_A_AT similar to fatty acid amide hydrolase gga
HG-U133_PLUS_2:204231_S_AT fatty acid amide hydrolase hs
HG-U133A:204231_S_AT fatty acid amide hydrolase hs
HG-FOCUS:204231_S_AT fatty acid amide hydrolase hs
HG-U133A_2:204231_S_AT fatty acid amide hydrolase hs
HG-U95AV2:34769_AT fatty acid amide hydrolase hs
HU35KSUBC:RC_AA435742_S_AT fatty acid amide hydrolase hs
HUGENEFL:U82535_AT fatty acid amide hydrolase hs
U133_X3P:G4557574_3P_S_AT fatty acid amide hydrolase hs
HG-U95AV2:39236_S_AT fatty acid amide hydrolase hs
MU11KSUBA:U82536_S_AT fatty acid amide hydrolase mm
MG-U74AV2:96795_AT fatty acid amide hydrolase mm
MG-U74BV2:113274_AT fatty acid amide hydrolase mm
MOE430A:1421969_A_AT fatty acid amide hydrolase mm
MOUSE430A_2:1421969_A_AT fatty acid amide hydrolase mm
MOUSE430_2:1421969_A_AT fatty acid amide hydrolase mm
MOE430B:1434091_AT fatty acid amide hydrolase mm
MOUSE430_2:1434091_AT fatty acid amide hydrolase mm
MU19KSUBB:TC26407_AT Fatty acid amide hydrolase, mRNA (cDNA clone MGC:11634 IMAGE:3595191) mm
RG-U34A:U72497_AT fatty acid amide hydrolase rn
RAE230A:1368717_AT fatty acid amide hydrolase rn
RAT230_2:1368717_AT fatty acid amide hydrolase rn
RAE230B:1384341_AT fatty acid amide hydrolase rn
RAT230_2:1384341_AT fatty acid amide hydrolase rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4040 amidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00665 Hypothetical protein B0218.2 [Caenorhabditis elegans] ref|NP_501366.1| B0218.2 [Caenorhabditis elegans] 0.0
blast CAE58425 Hypothetical protein CBG01556 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000120 EMBL-EBI Amidase 1.0E-126
Pfam IPR000120 EMBL-EBI Amidase 1.0E-126
Pfam IPR000120 EMBL-EBI Amidase 1.1E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501366.1 1 2-21
B0218.2.2 1 2-21
B0218.2.1 1 2-21
SNAP00000005553 1 2-21
GENEFINDER00000005562 1 2-21
Sequence
Target Sequence 
>C. ELEGANS:188343_AT
ggccaccaagagttcctgaagttttgagaatgtttttgagagcagtatgcgttgatgctg
gaacattcttgtctaagaagctgatgaatgacattatcgaccctcttctctacactcaag
ttgtactatggatggtgccagttccaattcaaagattcctttcaagcatcattaaagtct
tcttcccaagacttggaaatcttatggatgcaatgacgttgagtactgtggagcttcgta
atacatatgctgatattgaagcttatcgaagtgaaatggctggattgatggttgatcaga
agattgatgctcttctttgtccggttactgtatcgccagctcttccacatgtttctccat
ctaaattgtttgctggaactagttacactggaatcttcaatttattggattatgctgcag
gatctgttaatgtgactcatgttacaaaacaagatgaattggatcttgaagactatgaag
aatctgatccatggtatgctcttgcaaagaaaggatcacgtggaactattggtttcccaa
ttggagttcaagttgcaactcctccatacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCCACCAAGAGTTCCTGAAGTTTT 401 541 1100 Antisense
GCGTTGATGCTGGAACATTCTTGTC 528 291 1148 Antisense
TGAATGACATTATCGACCCTCTTCT 51 575 1184 Antisense
CCTCTTCTCTACACTCAAGTTGTAC 521 267 1201 Antisense
GGATGGTGCCAGTTCCAATTCAAAG 241 515 1229 Antisense
AAGATTGATGCTCTTCTTTGTCCGG 88 157 1399 Antisense
TTTGTCCGGTTACTGTATCGCCAGC 462 665 1415 Antisense
GTTTCTCCATCTAAATTGTTTGCTG 326 451 1450 Antisense
GCAGGATCTGTTAATGTGACTCATG 421 311 1516 Antisense
ATCTGATCCATGGTATGCTCTTGCA 251 33 1581 Antisense
TTCAAGTTGCAACTCCTCCATACAA 566 685 1646 Antisense
Sequence Source Affymetrix Proprietary Database