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[188342_at]

Description R02E12.8 /REP_DB=WormBase Gene ID /WP=CE23888 /GEN=acr-10 /TR=Q21645 /GB=AAA96188.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 61      4grp_total > 01      6grp_cgc > 84      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 72.5
(-0.1)
A 		33.1
(-3)
A 		74.1
(-0)
A 		115.4
(3)
A 		82
B 68.8
(3)
A 		59.2
(2)
A 		46.9
(0.7)
A 		11.4
(-3)
A 		57
C 22.3
(-3)
A 		52.2
(-0.4)
A 		49.4
(-0.7)
A 		91.9
(3)
M 		70
avr 54.5
(-1.5) 		48.2
(-3) 		56.8
(-0.9) 		72.9
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 93
(3)
P 		25.2
(-3)
A 		69
(0.9)
A 		31.1
(-2.5)
P 		45.9
(-1.2)
A 		61.2
(0.2)
A 		68
B 33.4
(-3)
A 		39.3
(-1.5)
P 		37.2
(-2.1)
A 		57.4
(3)
A 		56.8
(2.9)
A 		40.6
(-1.2)
A 		24
C 16.5
(-3)
A 		64
(1.8)
A 		62.9
(1.7)
A 		76.1
(3)
A 		58.7
(1.2)
A 		17.3
(-2.9)
A 		60
avr 47.6
(-0.2) 		42.8
(-1.9) 		56.4
(3) 		54.9
(2.5) 		53.8
(2.1) 		39.7
(-3) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 26.4
(3)
A 		7.2
(-3)
A 		19.4
(0.8)
A 		16.9
(0)
A 		19
B 21.9
(3)
A 		14.6
(0.8)
A 		10.9
(-0.4)
A 		2.4
(-3)
A 		20
C 7.4
(-3)
A 		15
(1.7)
A 		17.2
(3)
A 		15.5
(2)
M 		10
avr 18.6
(3) 		12.3
(-2.4) 		15.8
(0.6) 		11.6
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 17.2
(3)
P 		8.9
(-2.4)
A 		15
(1.6)
A 		7.9
(-3)
P 		13.9
(0.9)
A 		14.9
(1.5)
A 		9
B 11.6
(-1.9)
A 		13.1
(-1.3)
P 		9
(-3)
A 		15.2
(-0.4)
A 		23.1
(3)
A 		15.1
(-0.4)
A 		14
C 6.1
(-2.9)
A 		19.5
(3)
A 		16
(1.5)
A 		15.3
(1.2)
A 		19
(2.8)
A 		5.8
(-3)
A 		14
avr 11.6
(-3) 		13.8
(-1.1) 		13.3
(-1.6) 		12.8
(-2) 		18.7
(3) 		11.9
(-2.7) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188342_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18404
Sequence Type Exemplar sequence
Representative Public ID R02E12.8 NCBI
Target Description R02E12.8 /REP_DB=WormBase Gene ID /WP=CE23888 /GEN=acr-10 /TR=Q21645 /GB=AAA96188.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076291(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076291 NCBI Caenorhabditis elegans AcetylCholine Receptor family member (acr-10) (acr-10) mRNA, complete cds. 11/11 None
R02E12.8 ENSEMBL cdna:known chromosome:CEL140:X:4023289:4025559:1 gene:R02E12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4023289-4025560(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol acr-10
Ensembl R02E12.8
Entrez Gene 191598 Entrez gene
SwissProt Q21645 EMBL-EBI
WormBase CE23888 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
6811 ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
45211 postsynaptic membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4889 nicotinic acetylcholine-activated cation-selective channel activity inferred from electronic annotation QuickGO AmiGO
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5230 extracellular ligand-gated ion channel activity inferred from electronic annotation QuickGO AmiGO
30594 neurotransmitter receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA96188 Acetylcholine receptor protein 10 [Caenorhabditis elegans] ref|NP_508692.1| AcetylCholine Receptor family member (acr-10) [Caenorhabditis elegans] 0.0
blast CAE68515 Hypothetical protein CBG14329 [Caenorhabditis briggsae] 0.0
blast CAE59838 Hypothetical protein CBG03310 [Caenorhabditis briggsae] 1.0E-115
Protein Domains
Database ID Description E-Value
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 4.3E-10
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 5.0E-5
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 4.3E-69
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508692.1 5 181-203,213-230,243-265,280-302,485-507
R02E12.8 5 181-203,213-230,243-265,280-302,485-507
Sequence
Target Sequence 
>C. ELEGANS:188342_AT
aaacgccgagcaaccgttcaagctcgagaggaaatttccttttttgataatggtgatgat
gattgctgcccaaaacctcccgagttcgactattcactctccattgacagcgctgacact
ttaccgcttccacctcgtcctaagccactactcaagaagatgcataggttagtccagcac
atccatcaatcaaacttcgtacttttcaaacgttcccgccgaacatcttcatctcgacag
aaaagtcggcgacggaattctaagagccagcgacgaccgttggttttaacaaagaacgtc
aacaaaagtcattcattacgcagtcggcgtttcactcagtttgaaaagtatataaaaaag
tgcatagatgatgcaaaactcagaaaaaattcaacttattcgacgtattcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACGCCGAGCAACCGTTCAAGCTC 98 127 925 Antisense
GATGATGATTGCTGCCCAAAACCTC 103 411 979 Antisense
TGACAGCGCTGACACTTTACCGCTT 473 569 1029 Antisense
CGTCCTAAGCCACTACTCAAGAAGA 544 245 1060 Antisense
GCATAGGTTAGTCCAGCACATCCAT 345 307 1086 Antisense
CAGCACATCCATCAATCAAACTTCG 271 205 1099 Antisense
AAGTCGGCGACGGAATTCTAAGAGC 195 157 1167 Antisense
TAAGAGCCAGCGACGACCGTTGGTT 116 637 1185 Antisense
AAAGTCATTCATTACGCAGTCGGCG 46 123 1229 Antisense
TACGCAGTCGGCGTTTCACTCAGTT 144 647 1241 Antisense
AATTCAACTTATTCGACGTATTCAA 34 177 1312 Antisense
Sequence Source Affymetrix Proprietary Database