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[188338_at] helicase

Description F15D4.1 /REP_DB=WormBase Gene ID /WP=CE15856 /TR=O45899 /GB=CAB04949.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=helicase
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 57      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 81      6grp_total > 57      4grp_total > 36      6grp_highest_sub > Cu      6grp_cgc > 37      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 201
1 2 3 4 range
A 386.4
(1.1)
P 		434
(2.6)
P 		260.1
(-3)
P 		446.8
(3)
P 		187
B 371.8
(0.6)
P 		386.4
(1.6)
P 		405.9
(3)
P 		320.6
(-3)
P 		85
C 406.2
(3)
P 		341.7
(0.6)
P 		246.2
(-3)
P 		303
(-0.9)
P 		160
avr 388.1
(3) 		387.4
(3) 		304.1
(-3) 		356.8
(0.8) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 323
1 2 3 4 5 6 range
A 294.3
(-2.2)
P 		292.9
(-2.2)
P 		275.1
(-2.7)
P 		264.7
(-3)
P 		272.2
(-2.8)
P 		476.8
(3)
P 		212
B 305.3
(0.7)
P 		276.8
(-0.3)
P 		230.2
(-2)
P 		298.8
(0.5)
P 		204.2
(-3)
P 		366.6
(3)
P 		162
C 313.7
(1.2)
P 		256.6
(-0.3)
P 		352.3
(2.2)
P 		153.7
(-3)
P 		242.6
(-0.7)
P 		381.6
(3)
P 		228
avr 304.4
(-0.7) 		275.4
(-1.7) 		285.9
(-1.3) 		239.1
(-3) 		239.7
(-3) 		408.3
(3) 		169
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 140.7
(3)
P 		94.2
(-0.7)
P 		68
(-2.8)
P 		65.4
(-3)
P 		75
B 118.6
(3)
P 		95.4
(0.2)
P 		94.1
(0.1)
P 		68.3
(-3)
P 		50
C 134.5
(3)
P 		98.2
(0.4)
P 		85.8
(-0.5)
P 		51.1
(-3)
P 		83
avr 131.3
(3) 		95.9
(-0) 		82.6
(-1.2) 		61.6
(-3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 54.3
(-3)
P 		104.2
(1.8)
P 		59.9
(-2.5)
P 		67.1
(-1.8)
P 		82.2
(-0.3)
P 		116.1
(3)
P 		62
B 106.2
(0.7)
P 		92.1
(-0.3)
P 		55.9
(-3)
P 		79.1
(-1.3)
P 		83
(-1)
P 		136.7
(3)
P 		81
C 116.3
(2.3)
P 		78.3
(-0.1)
P 		89.7
(0.6)
P 		30.9
(-3)
P 		78.5
(-0)
P 		127.7
(3)
P 		97
avr 92.3
(-0.1) 		91.5
(-0.1) 		68.5
(-2.2) 		59
(-3) 		81.2
(-1) 		126.8
(3) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188338_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2625
Sequence Type Exemplar sequence
Representative Public ID F15D4.1 NCBI
Target Description F15D4.1 /REP_DB=WormBase Gene ID /WP=CE15856 /TR=O45899 /GB=CAB04949.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064401(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064401 NCBI Caenorhabditis elegans F15D4.1 (helicase) mRNA, complete cds. 11/11 None
SNAP00000038511 ENSEMBL cdna:SNAP chromosome:CEL140:II:13212797:13215329:1 11/11 None
GENEFINDER00000038523 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13212797:13215329:1 11/11 None
F15D4.1 ENSEMBL cdna:known chromosome:CEL140:II:13201670:13215592:1 gene:F15D4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13201666-13215326(+) 98.82 100.0
Public Domain and Genome References
Gene Title helicase
Gene Symbol btf-1
Ensembl F15D4.1
Entrez Gene 174965 Entrez gene
SwissProt O45899 EMBL-EBI
WormBase CE15856 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02491 Hypothetical protein F15D4.1 [Caenorhabditis elegans] emb|CAB04949.1| Hypothetical protein F15D4.1 [Caenorhabditis elegans] ref|NP_496802.1| F15D4.1 [Caenorhabditis elegans] 0.0
blast CAE59443 Hypothetical protein CBG02816 [Caenorhabditis briggsae] 0.0
blast XP_455023 unnamed protein product [Kluyveromyces lactis] emb|CAH00110.1| unnamed protein product [Kluyveromyces lactis NRRL Y-1140] 1.0E-131
Protein Domains
Database ID Description E-Value
Pfam IPR000330 EMBL-EBI SNF2 related domain 5.3E-22
Pfam IPR000330 EMBL-EBI SNF2 related domain 2.0E-40
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.0E-22
Pfam IPR000330 EMBL-EBI SNF2 related domain 2.3E-33
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.0E-22
Sequence
Target Sequence 
>C. ELEGANS:188338_AT
gagctctgattttctgccaatggaagacttcggcaaagctggtctctgatgctttgaaaa
gtggagaattcggaagtgttgtgtcgcatctggtccttgatggaagtgttccggcagggg
atcgcatgaaaatggtcaaccgcttcaatgaggataagacgattgatgtgttgattctga
cgacacatgtcggtggagttggtctgaacctaaccggagcggataccgtaatcttcctgg
accacgactggaacccgatgaaggatcttcaagccatcgatcgtgctcatcgtctcggcc
aaactcgaaatgtcaacgtatatcggctcatcacacagggaacagtcgaggagaaggtga
tgagcctggcaaagttcaagctgaatacggcccaggctttgatcggtgccgataatacat
caatgatgacaatggagacgggagaattgatgaatatgttcacgttggatggagatgaag
ccaagaagaagcctggcggtggtgagccggcagcgaagaagagtaaaaaatccaccggag
ctcccgaagaagtcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCTCTGATTTTCTGCCAATGGAA 484 387 3977 Antisense
GACTTCGGCAAAGCTGGTCTCTGAT 499 371 4002 Antisense
GGAAGTGTTGTGTCGCATCTGGTCC 71 533 4048 Antisense
GAGTTGGTCTGAACCTAACCGGAGC 31 375 4172 Antisense
CGGATACCGTAATCTTCCTGGACCA 83 243 4196 Antisense
GACCACGACTGGAACCCGATGAAGG 159 383 4216 Antisense
TCAAGCCATCGATCGTGCTCATCGT 278 631 4245 Antisense
TCATCGTCTCGGCCAAACTCGAAAT 400 619 4263 Antisense
GTCAACGTATATCGGCTCATCACAC 213 465 4288 Antisense
GAATACGGCCCAGGCTTTGATCGGT 273 327 4359 Antisense
TCCACCGGAGCTCCCGAAGAAGTCG 407 587 4507 Antisense
Sequence Source Affymetrix Proprietary Database