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[188337_at]

Description Y57A10A.11 /REP_DB=WormBase Gene ID /WP=CE22614 /GEN=rol-1 /TR=Q9NA83 /GB=CAB55014.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 18      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 10.2
(-0.4)
A 		16
(3)
A 		8.3
(-1.5)
A 		5.7
(-3)
A 		10
B 5.5
(-3)
A 		18.4
(3)
A 		9.1
(-1.3)
A 		5.6
(-3)
A 		13
C 5.4
(-3)
A 		7.8
(-2.7)
A 		6.7
(-2.8)
A 		53.4
(3)
A 		48
avr 7
(-3) 		14.1
(-0.1) 		8
(-2.6) 		21.6
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 666
1 2 3 4 5 6 range
A 7.6
(-3)
A 		21.1
(-2.8)
A 		10.6
(-3)
A 		195.2
(-0.8)
P 		531
(3)
P 		505.1
(2.7)
P 		523
B 21.6
(-2.8)
A 		4.6
(-3)
A 		36.9
(-2.7)
A 		45.6
(-2.6)
A 		539.1
(1.8)
P 		671
(3)
P 		666
C 4.9
(-3)
A 		13.9
(-2.9)
A 		6.9
(-3)
A 		223.1
(-1)
P 		670.8
(3)
P 		350.8
(0.1)
P 		666
avr 11.4
(-3) 		13.2
(-3) 		18.1
(-2.9) 		154.6
(-1.5) 		580.3
(3) 		509
(2.2) 		569
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 3.7
(3)
A 		3.5
(2.6)
A 		2.2
(-0.1)
A 		0.8
(-3)
A 		3
B 1.8
(-1.9)
A 		4.5
(3)
A 		2.1
(-1.4)
A 		1.2
(-3)
A 		3
C 1.8
(-3)
A 		2.2
(-2.7)
A 		2.3
(-2.6)
A 		9
(3)
A 		7
avr 2.4
(-2.2) 		3.4
(1.8) 		2.2
(-3) 		3.7
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 249
1 2 3 4 5 6 range
A 1.4
(-3)
A 		7.5
(-2.8)
A 		2.3
(-3)
A 		49.5
(-1.2)
P 		160.2
(3)
P 		123
(1.6)
P 		159
B 7.5
(-2.9)
A 		1.5
(-3)
A 		9
(-2.8)
A 		12.1
(-2.7)
A 		219
(2.2)
P 		250.2
(3)
P 		249
C 1.8
(-3)
A 		4.2
(-2.9)
A 		1.8
(-3)
A 		44.9
(-1.8)
P 		217.1
(3)
P 		117.4
(0.2)
P 		215
avr 3.6
(-3) 		4.4
(-3) 		4.4
(-3) 		35.5
(-2) 		198.8
(3) 		163.5
(1.9) 		195
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188337_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2861
Sequence Type Exemplar sequence
Representative Public ID Y57A10A.11 NCBI
Target Description Y57A10A.11 /REP_DB=WormBase Gene ID /WP=CE22614 /GEN=rol-1 /TR=Q9NA83 /GB=CAB55014.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064188(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064188 NCBI Caenorhabditis elegans ROLler: helically twisted, animals roll when moving family member (rol-1) (rol-1) mRNA, complete cds. 11/11 None
SNAP00000026020 ENSEMBL cdna:SNAP chromosome:CEL140:II:12165193:12175909:1 11/11 None
GENEFINDER00000026049 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12172503:12175909:1 11/11 None
Y57A10A.11 ENSEMBL cdna:known chromosome:CEL140:II:12172476:12175958:1 gene:Y57A10A.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12172499-12175906(+) 94.84 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rol-1
Ensembl Y57A10A.11
Entrez Gene 174857 Entrez gene
SwissProt Q9NA83 EMBL-EBI
WormBase CE22614 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6817 phosphate transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
42302 structural constituent of cuticle inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB55013 Hypothetical protein Y57A10A.8 [Caenorhabditis elegans] ref|NP_496588.2| Y57A10A.8 [Caenorhabditis elegans] 0.0
blast CAB55014 Hypothetical protein Y57A10A.11 [Caenorhabditis elegans] ref|NP_496589.1| ROLler: helically twisted, animals roll when moving family member (rol-1) [Caenorhabditis elegans] 1.0E-143
blast CAB55014 Hypothetical protein Y57A10A.11 [Caenorhabditis elegans] ref|NP_496589.1| ROLler: helically twisted, animals roll when moving family member (rol-1) [Caenorhabditis elegans] 1.0E-139
blast CAE73466 Hypothetical protein CBG20917 [Caenorhabditis briggsae] 1.0E-111
blast CAE73466 Hypothetical protein CBG20917 [Caenorhabditis briggsae] 1.0E-110
blast CAE73561 Hypothetical protein CBG21031 [Caenorhabditis briggsae] 2.0E-49
Protein Domains
Database ID Description E-Value
Pfam IPR002486 EMBL-EBI Nematode cuticle collagen, N-terminal 4.7E-7
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.2E-4
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.2E-8
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.0E-11
Pfam IPR006570 EMBL-EBI SPK 1.1E-35
Pfam IPR006570 EMBL-EBI SPK 1.9E-59
Pfam IPR002486 EMBL-EBI Nematode cuticle collagen, N-terminal 4.7E-7
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.2E-4
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.2E-8
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.0E-11
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496589.1 1 12-34
Y57A10A.11 1 12-34
GENEFINDER00000026049 1 12-34
Sequence
Target Sequence 
>C. ELEGANS:188337_AT
gtccagggcctccaggaaaagccggagccaacgggctcccaggaaactcgggaaaaccta
cgaaacgcccgtgcaacccggttaccaagccaccatgtgtcccatgtcctcaaggacccc
tcggacccgcaggaccagctggaccacaaggaaatcttggaggacctggggtgttcggtg
agaagggaccacaaggacccgtcggggcgcccggccaaaaggggcgacgcggggagaagg
gtaaggacggggagccaggagcccctggagcacctggagaaaatgtcaaggaggtcccag
cgatcccaggaccacaaggaactccaggtccacgtggaagacaaggaccacgtggagctc
caggaattcctggaaaagatgggcttggaggtgaccagggagctcctggagagcccggag
ccgatggagagccaggaatggatggagttcctggaaatccgggtagagatggaactcatg
gacacggaggagagaagggagtgtgtccaaagtattgttcggctgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCCAGGGCCTCCAGGAAAAGCCGG 351 475 827 Antisense
GGAGCCAACGGGCTCCCAGGAAACT 556 521 850 Antisense
ATCTTGGAGGACCTGGGGTGTTCGG 662 1 980 Antisense
GGGTGTTCGGTGAGAAGGGACCACA 619 361 995 Antisense
GCGCCCGGCCAAAAGGGGCGACGCG 317 277 1033 Antisense
AATGTCAAGGAGGTCCCAGCGATCC 339 163 1108 Antisense
GGAACTCCAGGTCCACGTGGAAGAC 163 529 1144 Antisense
GGGCTTGGAGGTGACCAGGGAGCTC 6 493 1207 Antisense
GGAGTTCCTGGAAATCCGGGTAGAG 666 515 1270 Antisense
GATGGAACTCATGGACACGGAGGAG 484 407 1294 Antisense
GTGTCCAAAGTATTGTTCGGCTGAT 223 487 1329 Antisense
Sequence Source Affymetrix Proprietary Database