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[188330_s_at]

Description F42A9.1B /REP_DB=WormBase Gene ID /WP=CE10294 /TR=Q20320 /GB=AAB03166.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=kinase
Cluster 6grp_total > 16      4grp_total > 38      6grp_cgc > 13      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 295
1 2 3 4 range
A 247.6
(3)
A 		79.6
(-1.4)
A 		21
(-3)
A 		81.2
(-1.4)
A 		227
B 264.5
(3)
P 		107.4
(-0.8)
A 		18.8
(-3)
A 		95
(-1.1)
A 		246
C 313.3
(3)
P 		72.8
(-2.1)
A 		31.3
(-3)
A 		67.2
(-2.2)
A 		282
avr 275.1
(3) 		86.6
(-1.5) 		23.7
(-3) 		81.1
(-1.6) 		251

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 356
1 2 3 4 5 6 range
A 277
(2.5)
A 		299.8
(3)
P 		7.5
(-3)
A 		125.8
(-0.6)
A 		71.2
(-1.7)
A 		85.2
(-1.4)
A 		292
B 322.4
(3)
P 		193.8
(0.1)
A 		71
(-2.7)
A 		104.8
(-1.9)
A 		56.2
(-3)
A 		59
(-2.9)
A 		266
C 363.4
(3)
P 		135.9
(-0.9)
A 		98.5
(-1.5)
A 		82.5
(-1.8)
A 		90.1
(-1.7)
A 		12.4
(-3)
A 		351
avr 320.9
(3) 		209.8
(0.5) 		59
(-2.8) 		104.4
(-1.8) 		72.5
(-2.5) 		52.2
(-3) 		269
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 90.2
(3)
A 		17.3
(-2.2)
A 		5.5
(-3)
A 		11.9
(-2.5)
A 		85
B 84.3
(3)
P 		26.5
(-1.3)
A 		4.4
(-3)
A 		20.2
(-1.8)
A 		80
C 103.7
(3)
P 		20.9
(-2.4)
A 		10.9
(-3)
A 		11.3
(-3)
A 		93
avr 92.7
(3) 		21.6
(-2) 		6.9
(-3) 		14.5
(-2.5) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 51.1
(-0.2)
A 		106.6
(3)
P 		1.6
(-3)
A 		31.9
(-1.3)
A 		21.5
(-1.9)
A 		20.7
(-1.9)
A 		105
B 112.2
(3)
P 		64.5
(-0)
A 		17.2
(-3)
A 		27.8
(-2.3)
A 		22.8
(-2.6)
A 		22
(-2.7)
A 		95
C 134.7
(3)
P 		41.5
(-1.3)
A 		25.1
(-2)
A 		16.6
(-2.4)
A 		29.2
(-1.9)
A 		4.2
(-3)
A 		131
avr 99.3
(3) 		70.9
(1) 		14.6
(-3) 		25.4
(-2.2) 		24.5
(-2.3) 		15.6
(-2.9) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188330_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8728
Sequence Type Exemplar sequence
Representative Public ID F42A9.1B NCBI
Target Description F42A9.1B /REP_DB=WormBase Gene ID /WP=CE10294 /TR=Q20320 /GB=AAB03166.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069075(11), NM_069076(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069075 NCBI Caenorhabditis elegans F42A9.1a (kinase) mRNA, complete cds. 11/11 None
NM_069076 NCBI Caenorhabditis elegans F42A9.1b (kinase) mRNA, complete cds. 11/11 None
F42A9.1a ENSEMBL cdna:known chromosome:CEL140:IV:8631415:8643986:1 gene:F42A9.1 11/11 A 
F42A9.1b ENSEMBL cdna:known chromosome:CEL140:IV:8631415:8643299:1 gene:F42A9.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000039869 8/11 Cross Hyb Matching Probes None
GENEFINDER00000039881 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8631420-8643305(+) 100.0 100.0
Public Domain and Genome References
Ensembl F42A9.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB03166 Temporarily assigned gene name protein 137, isoform a [Caenorhabditis elegans] ref|NP_501476.1| F42A9.1a [Caenorhabditis elegans] 0.0
blast AAB03165 Temporarily assigned gene name protein 137, isoform b [Caenorhabditis elegans] ref|NP_501477.1| F42A9.1b [Caenorhabditis elegans] 0.0
blast CAE60006 Hypothetical protein CBG03505 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 3.1E-62
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 1.1E-11
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 8.3E-57
Pfam IPR001660 EMBL-EBI Sterile alpha motif SAM 2.2E-24
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 8.3E-57
Pfam IPR001660 EMBL-EBI Sterile alpha motif SAM 2.2E-24
Sequence
Target Sequence 
>C. ELEGANS:188330_S_AT
aagatcccgattcactgcatggtttcacaaacgatttggaactagatctgcaccatttgc
attcgctaatattccatactggacgagcgaagaagtatgtgcatggctctcttcaattgg
aatgtctgaatatggatcaacgttccgaaagaatgatattcaaggttctgaattgatgca
tttagaacgatccgatattatggatattgggatcacaaaaattggacatgtgaagaggtt
gcagtccgccattgtcgacctccgagctcaaaaccaacgtgcacgtcgcgctcaggctcg
taaaaaacgtgtcgccaaagactataaacccgatcattctggtccttctggtgctggcgg
tgcagtagataaacgaaaagaatcaagtgctgaaagatatgcagatggacaaccatcaac
atcatctacgggagctaccacttcatctggaaaaacacggagaattccaccgaaagatgg
agaatccgcgcctgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGATCCCGATTCACTGCATGGTTT 481 155 3447 Antisense
AGATCTGCACCATTTGCATTCGCTA 440 67 3490 Antisense
GCATTCGCTAATATTCCATACTGGA 525 307 3505 Antisense
AGTATGTGCATGGCTCTCTTCAATT 338 63 3540 Antisense
TGTGAAGAGGTTGCAGTCCGCCATT 206 557 3675 Antisense
TCAAAACCAACGTGCACGTCGCGCT 506 627 3714 Antisense
CGTCGCGCTCAGGCTCGTAAAAAAC 78 245 3730 Antisense
TAAACCCGATCATTCTGGTCCTTCT 484 639 3771 Antisense
TTCTGGTGCTGGCGGTGCAGTAGAT 400 695 3792 Antisense
ACATCATCTACGGGAGCTACCACTT 657 105 3865 Antisense
GAAAGATGGAGAATCCGCGCCTGCT 133 361 3918 Antisense
Sequence Source Affymetrix Proprietary Database