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[188328_at] annexin

Description C37H5.1 /REP_DB=WormBase Gene ID /WP=CE08625 /TR=P91144 /GB=AAB42365.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=annexin
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 17      4grp_highest_8_8 > 52      4grp_high_8_8 > 25      6grp_highest_nonorm > 60      6grp_total > 17      4grp_total > 39      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 594
1 2 3 4 range
A 633.7
(3)
P 		204.2
(-2)
P 		115.7
(-3)
P 		357.5
(-0.2)
P 		518
B 706
(3)
P 		195.5
(-2.2)
P 		111.9
(-3)
P 		347.7
(-0.6)
P 		594
C 701.5
(3)
P 		242.9
(-1.9)
P 		142.6
(-3)
P 		357.8
(-0.7)
P 		559
avr 680.4
(3) 		214.2
(-2) 		123.4
(-3) 		354.3
(-0.5) 		557

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 505
1 2 3 4 5 6 range
A 586.4
(3)
P 		433
(0.5)
P 		240.4
(-2.6)
P 		240
(-2.6)
P 		216.6
(-3)
P 		214.9
(-3)
P 		372
B 664.9
(3)
P 		346.2
(-0.9)
P 		296.9
(-1.5)
P 		204.7
(-2.6)
P 		172.9
(-3)
P 		266.4
(-1.9)
P 		492
C 578
(3)
P 		393.2
(0.3)
P 		316.2
(-0.8)
P 		159.7
(-3)
P 		242.9
(-1.8)
P 		205.4
(-2.3)
P 		418
avr 609.8
(3) 		390.8
(-0.2) 		284.5
(-1.8) 		201.5
(-3) 		210.8
(-2.9) 		228.9
(-2.6) 		408
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 206
1 2 3 4 range
A 230.7
(3)
P 		44.3
(-2.6)
P 		30.2
(-3)
P 		52.3
(-2.3)
P 		201
B 225.1
(3)
P 		48.3
(-2.3)
P 		25.9
(-3)
P 		74.1
(-1.5)
P 		199
C 232.2
(3)
P 		69.8
(-2.3)
P 		49.7
(-3)
P 		60.3
(-2.7)
P 		183
avr 229.3
(3) 		54.1
(-2.4) 		35.3
(-3) 		62.2
(-2.2) 		194

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 199
1 2 3 4 5 6 range
A 108.1
(0.3)
P 		154
(3)
P 		52.4
(-3)
P 		60.9
(-2.5)
P 		65.4
(-2.2)
P 		52.3
(-3)
P 		102
B 231.4
(3)
P 		115.2
(-0.9)
P 		72.1
(-2.4)
P 		54.2
(-3)
P 		70.2
(-2.5)
P 		99.3
(-1.5)
P 		177
C 214.3
(3)
P 		120
(-0.1)
P 		80.5
(-1.4)
P 		32.1
(-3)
P 		78.6
(-1.5)
P 		68.8
(-1.8)
P 		182
avr 184.6
(3) 		129.7
(0.6) 		68.3
(-2.1) 		49.1
(-3) 		71.4
(-2) 		73.5
(-1.9) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188328_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14864
Sequence Type Exemplar sequence
Representative Public ID C37H5.1 NCBI
Target Description C37H5.1 /REP_DB=WormBase Gene ID /WP=CE08625 /TR=P91144 /GB=AAB42365.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=annexin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071899(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071899 NCBI Caenorhabditis elegans C37H5.1 (annexin) mRNA, complete cds. 11/11 None
SNAP00000016590 ENSEMBL cdna:SNAP chromosome:CEL140:V:4855199:4856777:1 11/11 None
GENEFINDER00000016603 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4847573:4856777:1 11/11 None
C37H5.1 ENSEMBL cdna:known chromosome:CEL140:V:4855139:4857074:1 gene:C37H5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4855198-4856777(+) 100.0 100.0
Public Domain and Genome References
Gene Title annexin
Gene Symbol nex-4
Ensembl C37H5.1
Entrez Gene 183294 Entrez gene
SwissProt P91144 EMBL-EBI
WormBase CE08625 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
50819 negative regulation of coagulation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
5544 calcium-dependent phospholipid binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42365 Annexin family protein 4 [Caenorhabditis elegans] ref|NP_504300.1| C37H5.1 [Caenorhabditis elegans] 0.0
blast AAB42366 Hypothetical protein C37H5.2 [Caenorhabditis elegans] ref|NP_504299.2| C37H5.2 [Caenorhabditis elegans] 0.0
blast CAE64207 Hypothetical protein CBG08840 [Caenorhabditis briggsae] 1.0E-167
blast AAB42365 Annexin family protein 4 [Caenorhabditis elegans] ref|NP_504300.1| C37H5.1 [Caenorhabditis elegans] 1.0E-164
blast AAB42367 Hypothetical protein C37H5.3a [Caenorhabditis elegans] ref|NP_504297.1| C37H5.3a [Caenorhabditis elegans] 1.0E-152
blast BAE29257.1 unnamed protein product [Mus musculus] 3.0E-31
Protein Families
Method ID Description E-Value
ec 6PGL_DROME (Q9VZ64) Potential 6-phosphogluconolactonase (EC 3.1.1.31) (6PGL) 1.4
Protein Domains
Database ID Description E-Value
Pfam IPR001464 EMBL-EBI Annexin 1.5E-13
Pfam IPR001464 EMBL-EBI Annexin 1.6E-13
Pfam IPR001464 EMBL-EBI Annexin 3.5E-23
Pfam IPR000073 EMBL-EBI Alpha/beta hydrolase fold 4.6E-23
Pfam IPR001464 EMBL-EBI Annexin 8.5E-10
Pfam IPR001464 EMBL-EBI Annexin 1.6E-13
Pfam IPR001464 EMBL-EBI Annexin 3.5E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000016603 3 12-34,49-71,123-140
Sequence
Target Sequence 
>C. ELEGANS:188328_AT
aaatgatgcatctccttctgtgcaacaaccgcgaggagggcctcggagtccgtgtgaatg
atgagcttgtcgacaagcacgtcaaaatcttgatgaccactaccatcgatgatatcgcga
agaatgtaaacttattcgaagaacttttcatcggacactcatggaaacatattggaacag
tcttggacaaggttgatgaagcccgcaaagacgttcaagacattgaaagtatcattcgtc
gcaacaaaaatattcactccgagattcggctgattctattgacgattgctcgcgtttcac
gaaatattcaaatctatttcgctgaaaaactccgaacagcaatgaccgcagaacgtgtcg
accagtcaacgattattcggatttgcgttagtcgtagtgagattgacttacaagacatct
ctctcgagtacaagcgaaaatatcaacgccctctggagcacgacatttgccaatcaactt
ctggggaatatacccgaatgatttgcacacttctcagcggattcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATGATGCATCTCCTTCTGTGCAA 490 119 494 Antisense
CTGTGCAACAACCGCGAGGAGGGCC 543 239 511 Antisense
TGACCACTACCATCGATGATATCGC 548 569 587 Antisense
GAAGAACTTTTCATCGGACACTCAT 369 339 631 Antisense
TATTGACGATTGCTCGCGTTTCACG 484 665 770 Antisense
AAACTCCGAACAGCAATGACCGCAG 520 127 820 Antisense
ATGACCGCAGAACGTGTCGACCAGT 261 45 835 Antisense
GACTTACAAGACATCTCTCTCGAGT 614 371 898 Antisense
CGAAAATATCAACGCCCTCTGGAGC 165 247 928 Antisense
GGAGCACGACATTTGCCAATCAACT 372 519 948 Antisense
TTTGCACACTTCTCAGCGGATTCAA 150 669 995 Antisense
Sequence Source Affymetrix Proprietary Database