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[188327_at]

Description ZK84.6 /REP_DB=WormBase Gene ID /WP=CE15253 /GEN=ins-6 /TR=SW:P56174 /GB=AAC48208.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 57      4grp_highest_8_8 > 07      4grp_high_8_8 > 21      6grp_total > 59      4grp_total > 18      6grp_cgc > 28      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1013
1 2 3 4 range
A 208.5
(-3)
P 		467.6
(-1.4)
P 		293.8
(-2.5)
P 		1195.8
(3)
P 		987
B 182.9
(-3)
P 		416
(-1.6)
P 		281.4
(-2.4)
P 		1162.9
(3)
P 		980
C 212.1
(-3)
P 		395.6
(-1.7)
P 		314.4
(-2.3)
P 		1078.4
(3)
P 		866
avr 201.2
(-3) 		426.4
(-1.6) 		296.5
(-2.4) 		1145.7
(3) 		945

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 303
1 2 3 4 5 6 range
A 282.3
(1.9)
P 		67.2
(-3)
A 		113.8
(-1.9)
P 		130.3
(-1.6)
P 		146.1
(-1.2)
P 		329.2
(3)
P 		262
B 225
(0.4)
P 		134.6
(-2.2)
P 		106.1
(-3)
P 		116.2
(-2.7)
P 		126.7
(-2.4)
P 		315.6
(3)
P 		210
C 210.8
(-0.5)
P 		105.1
(-2.8)
A 		96
(-3)
A 		112.3
(-2.6)
M 		129.3
(-2.3)
P 		370.3
(3)
P 		274
avr 239.4
(0.5) 		102.3
(-3) 		105.3
(-2.9) 		119.6
(-2.6) 		134
(-2.2) 		338.4
(3) 		236
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 75.9
(-3)
P 		101.5
(-1.5)
P 		76.8
(-2.9)
P 		175
(3)
P 		99
B 58.3
(-3)
P 		102.7
(-1.6)
P 		65.2
(-2.8)
P 		247.8
(3)
P 		190
C 70.2
(-3)
P 		113.7
(-0.7)
P 		109.6
(-0.9)
P 		181.8
(3)
P 		112
avr 68.1
(-3) 		106
(-1.3) 		83.9
(-2.3) 		201.5
(3) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 52
(0)
P 		23.9
(-3)
A 		24.8
(-2.9)
P 		33.1
(-2)
P 		44.1
(-0.8)
P 		80.1
(3)
P 		56
B 78.3
(0.4)
P 		44.8
(-1.8)
P 		25.8
(-3)
P 		30.8
(-2.7)
P 		51.5
(-1.3)
P 		117.7
(3)
P 		92
C 78.1
(0.3)
P 		32.1
(-2.4)
A 		24.4
(-2.9)
A 		22.6
(-3)
M 		41.8
(-1.9)
P 		124
(3)
P 		101
avr 69.5
(0.2) 		33.6
(-2.4) 		25
(-3) 		28.8
(-2.7) 		45.8
(-1.5) 		107.3
(3) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188327_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5163
Sequence Type Exemplar sequence
Representative Public ID ZK84.6 NCBI
Target Description ZK84.6 /REP_DB=WormBase Gene ID /WP=CE15253 /GEN=ins-6 /TR=SW:P56174 /GB=AAC48208.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062797(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062797 NCBI Caenorhabditis elegans INSulin related family member (ins-6) (ins-6) mRNA, complete cds. 11/11 None
SNAP00000003781 ENSEMBL cdna:SNAP chromosome:CEL140:II:6000449:6000841:1 11/11 None
GENEFINDER00000003790 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6000449:6000841:1 11/11 None
ZK84.6 ENSEMBL cdna:known chromosome:CEL140:II:6000449:6000841:1 gene:ZK84.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6000445-6000838(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-6
Ensembl ZK84.6
Entrez Gene 191687 Entrez gene
SwissProt P56174 EMBL-EBI
WormBase CE15253 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7582 physiological process inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC48208 Insulin related protein 6 [Caenorhabditis elegans] ref|NP_495198.1| INSulin related family member (ins-6) [Caenorhabditis elegans] sp|P56174|ILB5_CAEEL Probable insulin-like peptide beta-type 5 precursor 3.0E-50
blast CAE59234 Hypothetical protein CBG02555 [Caenorhabditis briggsae] 3.0E-17
blast AAC46744 Insulin related protein 4 [Caenorhabditis elegans] ref|NP_495196.1| INSulin related family member (ins-4) [Caenorhabditis elegans] sp|Q09626|ILB1_CAEEL Probable insulin-like peptide beta-type 1 precursor 1.0E-16
Protein Domains
Database ID Description E-Value
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 1.3E-40
Sequence
Target Sequence 
>C. ELEGANS:188327_AT
aactctgtctttactatcatcttcgttttgtgcgcactccaagtcgctgcaagtttccgt
caatccttcggtccttcaatgtctgaagaatcagcaagcatgcaacttctccgtgaactt
caacacaacatgatggaatcagctcaccgaccaatgccacgagcaagacgtgttccagca
ccaggagaaactcgtgcctgcggaagaaaactcatctctttagtcatggctgtctgtgga
gatctttgcaacccacaagaaggaaaggacattgcgactgaatgctgcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTCTGTCTTTACTATCATCTTCG 517 141 16 Antisense
ACTATCATCTTCGTTTTGTGCGCAC 566 101 28 Antisense
TGCAAGTTTCCGTCAATCCTTCGGT 278 573 63 Antisense
TCCTTCGGTCCTTCAATGTCTGAAG 133 585 79 Antisense
ATCAGCAAGCATGCAACTTCTCCGT 89 29 105 Antisense
ACCGACCAATGCCACGAGCAAGACG 485 89 161 Antisense
GACGTGTTCCAGCACCAGGAGAAAC 162 377 182 Antisense
GAGAAACTCGTGCCTGCGGAAGAAA 189 393 200 Antisense
ATCTCTTTAGTCATGGCTGTCTGTG 663 31 229 Antisense
GCTGTCTGTGGAGATCTTTGCAACC 554 295 244 Antisense
GGACATTGCGACTGAATGCTGCGGA 702 525 282 Antisense
Sequence Source Affymetrix Proprietary Database