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[188319_at]

Description ZK84.3 /REP_DB=WormBase Gene ID /WP=CE17309 /GEN=ins-5 /TR=SW:P56173 /GB=AAC48209.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 52      4grp_total > 52      6grp_cgc > 92      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 900
1 2 3 4 range
A 587.9
(1.1)
P 		792.3
(3)
P 		555.4
(0.8)
P 		149.4
(-3)
A 		643
B 682.7
(1.4)
P 		924.2
(3)
P 		530.2
(0.4)
P 		23.9
(-3)
A 		900
C 682.9
(2)
P 		814
(3)
P 		603.3
(1.3)
P 		54.6
(-3)
A 		759
avr 651.2
(1.5) 		843.5
(3) 		563
(0.8) 		76
(-3) 		768

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 305
1 2 3 4 5 6 range
A 68
(-2.4)
A 		191.4
(0.1)
P 		332.7
(3)
P 		117.4
(-1.4)
A 		165.8
(-0.4)
P 		39.8
(-3)
A 		293
B 159
(-0.7)
A 		209
(0.5)
A 		318.8
(3)
P 		208.9
(0.5)
M 		133.9
(-1.2)
A 		57
(-3)
A 		262
C 89
(-2.8)
A 		173.6
(-0.9)
A 		345.2
(3)
P 		263.8
(1.2)
P 		80.9
(-3)
A 		90.9
(-2.8)
A 		264
avr 105.3
(-2) 		191.3
(-0.1) 		332.2
(3) 		196.7
(-0) 		126.9
(-1.6) 		62.6
(-3) 		270
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 229
1 2 3 4 range
A 214.1
(3)
P 		172
(1.7)
P 		145.2
(0.8)
P 		21.9
(-3)
A 		192
B 217.7
(2.7)
P 		228.3
(3)
P 		122.9
(0.2)
P 		5.1
(-3)
A 		223
C 226.1
(2.8)
P 		234
(3)
P 		210.3
(2.4)
P 		9.2
(-3)
A 		225
avr 219.3
(3) 		211.4
(2.8) 		159.5
(1.3) 		12.1
(-3) 		207

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 12.5
(-2.7)
A 		68.1
(2.6)
P 		72.4
(3)
P 		29.8
(-1.1)
A 		50
(0.9)
P 		9.7
(-3)
A 		63
B 55.3
(0.6)
A 		69.6
(2.2)
A 		77.4
(3)
P 		55.3
(0.6)
M 		54.4
(0.5)
A 		21.2
(-3)
A 		56
C 33
(-2.3)
A 		53
(-0.4)
A 		87.9
(3)
P 		53.1
(-0.4)
P 		26.2
(-3)
A 		30.4
(-2.6)
A 		62
avr 33.6
(-1.7) 		63.6
(1.4) 		79.2
(3) 		46.1
(-0.4) 		43.5
(-0.6) 		20.4
(-3) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188319_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4969
Sequence Type Exemplar sequence
Representative Public ID ZK84.3 NCBI
Target Description ZK84.3 /REP_DB=WormBase Gene ID /WP=CE17309 /GEN=ins-5 /TR=SW:P56173 /GB=AAC48209.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062796(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062796 NCBI Caenorhabditis elegans INSulin related family member (ins-5) (ins-5) mRNA, complete cds. 11/11 None
SNAP00000003780 ENSEMBL cdna:SNAP chromosome:CEL140:II:5998442:5998808:1 11/11 None
ZK84.3 ENSEMBL cdna:known chromosome:CEL140:II:5998442:5998791:1 gene:ZK84.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000003787 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5998438-5998789(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-5
Ensembl ZK84.3
Entrez Gene 191686 Entrez gene
SwissProt P56173 EMBL-EBI
WormBase CE36081 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495197 INSulin related family member (ins-5) [Caenorhabditis elegans] 7.0E-42
blast AAC48209 Insulin related protein 5 [Caenorhabditis elegans] 5.0E-41
blast P56173 Putative insulin-like peptide beta-type 2.0E-34
blast NP_495197 INSulin related family member (ins-5) [Caenorhabditis elegans] 2.0E-31
blast P56173 Putative insulin-like peptide beta-type 3.0E-31
blast T29013 hypothetical protein ZK84.3 - Caenorhabditis elegans 4.0E-28
Sequence
Target Sequence 
>C. ELEGANS:188319_AT
gtcgccttgatgctcatcggaacaattctcccaatcgctgctcttcaccagaagcatcaa
ggcttcatcctgtcgtcatccgattcaaccggaaaccaaccaatggatgcgatctcaaga
gccgaccgtcacaccaactaccgatcatgcgcattgggctcatcccgcatgtctgaaaac
ggaatcgatgtcgctcaaaaatgttgctccactgattgcagctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGCCTTGATGCTCATCGGAACAA 544 473 25 Antisense
CATCGGAACAATTCTCCCAATCGCT 665 211 39 Antisense
TCGCTGCTCTTCACCAGAAGCATCA 478 599 59 Antisense
AAGCATCAAGGCTTCATCCTGTCGT 375 151 76 Antisense
CCTGTCGTCATCCGATTCAACCGGA 587 265 93 Antisense
GATTCAACCGGAAACCAACCAATGG 472 419 106 Antisense
TGGATGCGATCTCAAGAGCCGACCG 196 551 128 Antisense
ACACCAACTACCGATCATGCGCATT 542 109 155 Antisense
GCTCATCCCGCATGTCTGAAAACGG 201 301 182 Antisense
GATGTCGCTCAAAAATGTTGCTCCA 344 409 211 Antisense
AATGTTGCTCCACTGATTGCAGCTC 166 163 224 Antisense
Sequence Source Affymetrix Proprietary Database