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[188314_at]

Description ZK75.1 /REP_DB=WormBase Gene ID /WP=CE02100 /GEN=ins-4 /TR=SW:Q09626 /GB=AAC46744.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 04      6grp_highest_nonorm > 80      6grp_total > 33      4grp_total > 50      6grp_highest_sub > De      6grp_cgc > 80      6grp_high1_sub > De      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 291
1 2 3 4 range
A 113.6
(-0.9)
P 		270
(3)
P 		241.1
(2.3)
P 		28.3
(-3)
A 		242
B 147
(-1.3)
P 		294.6
(3)
P 		205.7
(0.4)
P 		90.9
(-3)
M 		204
C 144.6
(-1.7)
P 		319.4
(3)
P 		220.3
(0.4)
P 		94.7
(-3)
A 		225
avr 135.1
(-1.3) 		294.7
(3) 		222.4
(1.1) 		71.3
(-3) 		223

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 255.7
(1.7)
P 		224.7
(0.5)
P 		286.8
(3)
P 		157.5
(-2.2)
P 		253.6
(1.7)
P 		137.7
(-3)
P 		149
B 249.1
(3)
P 		229.7
(1.5)
P 		236.3
(2)
P 		208.2
(-0.1)
P 		241.7
(2.4)
P 		169.1
(-3)
P 		80
C 216.1
(1.2)
P 		246.4
(3)
P 		204.3
(0.5)
P 		157
(-2.4)
P 		147
(-3)
P 		191.8
(-0.3)
P 		99
avr 240.3
(2.8) 		233.6
(2.3) 		242.5
(3) 		174.2
(-2.4) 		214.1
(0.8) 		166.2
(-3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 41.4
(0.8)
P 		58.6
(2.6)
P 		63
(3)
P 		4.1
(-3)
A 		59
B 46.9
(0.1)
P 		72.8
(3)
P 		47.7
(0.2)
P 		19.4
(-3)
M 		53
C 47.9
(-0.5)
P 		91.8
(3)
P 		76.8
(1.8)
P 		16
(-3)
A 		76
avr 45.4
(0.2) 		74.4
(3) 		62.5
(1.8) 		13.2
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 47.1
(-1.2)
P 		79.9
(3)
P 		62.5
(0.8)
P 		39.9
(-2.2)
P 		76.5
(2.6)
P 		33.5
(-3)
P 		46
B 86.7
(1.4)
P 		76.5
(-0)
P 		57.4
(-2.7)
P 		55.1
(-3)
P 		98.2
(3)
P 		63.1
(-1.9)
P 		43
C 80.1
(3)
P 		75.2
(2.4)
P 		52
(-0.5)
P 		31.6
(-3)
P 		47.6
(-1)
P 		64.2
(1)
P 		49
avr 71.3
(2) 		77.2
(3) 		57.3
(-0.4) 		42.2
(-3) 		74.1
(2.5) 		53.6
(-1) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188314_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4786
Sequence Type Exemplar sequence
Representative Public ID ZK75.1 NCBI
Target Description ZK75.1 /REP_DB=WormBase Gene ID /WP=CE02100 /GEN=ins-4 /TR=SW:Q09626 /GB=AAC46744.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062795(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062795 NCBI Caenorhabditis elegans INSulin related family member (ins-4) (ins-4) mRNA, complete cds. 11/11 None
SNAP00000003779 ENSEMBL cdna:SNAP chromosome:CEL140:II:5996465:5996732:1 9/11 None
GENEFINDER00000003792 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5996465:5996732:1 9/11 None
ZK75.1 ENSEMBL cdna:known chromosome:CEL140:II:5996354:5996732:1 gene:ZK75.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5996350-5996729(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-4
Ensembl ZK75.1
Entrez Gene 191685 Entrez gene
SwissProt Q09626 EMBL-EBI
WormBase CE02100 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7582 physiological process inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46744 Insulin related protein 4 [Caenorhabditis elegans] ref|NP_495196.1| INSulin related family member (ins-4) [Caenorhabditis elegans] sp|Q09626|ILB1_CAEEL Probable insulin-like peptide beta-type 1 precursor 2.0E-47
blast AAC46744 Insulin related protein 4 [Caenorhabditis elegans] ref|NP_495196.1| INSulin related family member (ins-4) [Caenorhabditis elegans] sp|Q09626|ILB1_CAEEL Probable insulin-like peptide beta-type 1 precursor 3.0E-36
blast CAB61047 Hypothetical protein Y116F11B.1 [Caenorhabditis elegans] ref|NP_507840.1| abnormal DAuer Formation family member (daf-28) [Caenorhabditis elegans] 2.0E-18
blast CAE59233 Hypothetical protein CBG02554 [Caenorhabditis briggsae] 4.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 4.4E-42
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 4.4E-42
Sequence
Target Sequence 
>C. ELEGANS:188314_AT
tcattctttacatatttccttctctccgcacttcttctctccgcttcatgtcgacaacct
tccatggacaccagcaaagccgatcgtattctacgagagatcgaaatggaaacagaactc
gaaaatcaactctcccgagcacgacgagtcccagctggagaggttcgtgcctgtggaaga
cgacttcttctctttgtctggtcaacctgtggagaaccatgcacgccacaagaggacatg
gacattgccacagtttgctgcacaacacagtgcactccatcata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATTCTTTACATATTTCCTTCTCT 247 619 19 Antisense
GACACCAGCAAAGCCGATCGTATTC 577 365 85 Antisense
GACGAGTCCCAGCTGGAGAGGTTCG 192 379 161 Antisense
GAGAGGTTCGTGCCTGTGGAAGACG 31 395 176 Antisense
GGAAGACGACTTCTTCTCTTTGTCT 665 529 193 Antisense
TTCTCTTTGTCTGGTCAACCTGTGG 121 689 206 Antisense
CAACCTGTGGAGAACCATGCACGCC 506 187 221 Antisense
AACCATGCACGCCACAAGAGGACAT 48 149 233 Antisense
GAGGACATGGACATTGCCACAGTTT 189 405 250 Antisense
GCCACAGTTTGCTGCACAACACAGT 411 279 265 Antisense
GCACAACACAGTGCACTCCATCATA 537 317 278 Antisense
Sequence Source Affymetrix Proprietary Database