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[188308_s_at] DnaJ

Description F44E2.3 /REP_DB=WormBase Gene ID /WP=CE00181 /TR=SW:P34433 /GB=AAA28041.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=DnaJ
Cluster 6grp_highest_10_10 > 53      6grp_high1_10_10 > 35      4grp_highest_8_8 > 45      4grp_high_8_8 > 09      6grp_highest_nonorm > 81      6grp_total > 36      4grp_total > 23      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 227.6
(1.4)
P 		109.8
(-3)
P 		124.2
(-2.5)
P 		268.9
(3)
P 		159
B 297.4
(3)
P 		101.2
(-3)
P 		126.2
(-2.2)
P 		228.5
(0.9)
P 		196
C 297.5
(3)
P 		107.8
(-3)
P 		124
(-2.5)
P 		222.6
(0.6)
P 		190
avr 274.2
(3) 		106.3
(-3) 		124.8
(-2.3) 		240
(1.8) 		168

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 173
1 2 3 4 5 6 range
A 282.3
(0.4)
P 		349
(3)
P 		254.1
(-0.7)
P 		202.7
(-2.7)
P 		194.6
(-3)
P 		268.6
(-0.1)
P 		154
B 284.6
(3)
P 		283.3
(2.9)
P 		186.2
(-3)
P 		199.1
(-2.2)
P 		231.2
(-0.3)
P 		226
(-0.6)
P 		98
C 260.8
(0.8)
P 		310.7
(3)
P 		261.5
(0.8)
P 		199.6
(-1.9)
P 		175.8
(-3)
P 		294.2
(2.3)
P 		135
avr 275.9
(1) 		314.3
(3) 		233.9
(-1.2) 		200.5
(-3) 		200.5
(-3) 		262.9
(0.3) 		114
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 82.9
(3)
P 		23.8
(-3)
P 		32.5
(-2.1)
P 		39.4
(-1.4)
P 		59
B 94.8
(3)
P 		25
(-3)
P 		29.2
(-2.6)
P 		48.7
(-1)
P 		70
C 98.5
(3)
P 		31
(-3)
P 		43.2
(-1.9)
P 		37.5
(-2.4)
P 		68
avr 92.1
(3) 		26.6
(-3) 		35
(-2.2) 		41.9
(-1.6) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 52
(-3)
P 		124.1
(3)
P 		55.3
(-2.7)
P 		51.4
(-3)
P 		58.7
(-2.4)
P 		65.4
(-1.8)
P 		73
B 99
(3)
P 		94.3
(2.5)
P 		45.2
(-3)
P 		52.7
(-2.2)
P 		93.9
(2.4)
P 		84.3
(1.4)
P 		54
C 96.7
(2.8)
P 		94.8
(2.6)
P 		66.6
(-0.3)
P 		40.1
(-3)
P 		56.9
(-1.3)
P 		98.5
(3)
P 		58
avr 82.6
(0.7) 		104.4
(3) 		55.7
(-2.2) 		48.1
(-3) 		69.8
(-0.7) 		82.7
(0.7) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188308_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7310
Sequence Type Exemplar sequence
Representative Public ID F44E2.3 NCBI
Target Description F44E2.3 /REP_DB=WormBase Gene ID /WP=CE00181 /TR=SW:P34433 /GB=AAA28041.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=DnaJ
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066549(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066549 NCBI Caenorhabditis elegans F44E2.3 (DnaJ) mRNA, complete cds. 11/11 None
SNAP00000039998 ENSEMBL cdna:SNAP chromosome:CEL140:III:8827399:8829189:-1 11/11 None
GENEFINDER00000040009 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8827399:8829189:-1 11/11 None
F44E2.3 ENSEMBL cdna:known chromosome:CEL140:III:8827313:8829189:-1 gene:F44E2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8827394-8829185(-) 100.0 100.0
Public Domain and Genome References
Gene Title DnaJ
Ensembl F44E2.3
Entrez Gene 176242 Entrez gene
SwissProt P34433 EMBL-EBI
WormBase CE00181 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA28041 Hypothetical protein F44E2.3 [Caenorhabditis elegans] ref|NP_498950.1| F44E2.3 [Caenorhabditis elegans] sp|P34433|YL53_CAEEL Hypothetical protein F44E2.3 in chromosome III 6.0E-85
blast CAE62740 Hypothetical protein CBG06903 [Caenorhabditis briggsae] 2.0E-78
blast XP_877872 PREDICTED: hypothetical protein XP_872779 isoform 4 [Bos taurus] emb|CAI13168.1| RP11-297I6.1 [Homo sapiens] gb|AAH50434.1| FLJ10154 protein [Homo sapiens] dbj|BAA91467.1| unnamed protein product [Homo sapiens] 2.0E-12
Sequence
Target Sequence 
>C. ELEGANS:188308_S_AT
aaacggaggcatcgaagctcatcgtcagaaggttcacaagccgaaccacatcaattggga
agtattttcagagaagagagacgtcgacgagagagaaatgaatctccaaaacttccacct
cctcctccaccaccaccgtcggatcccccagttgacacaagcatcccattcgacgtgagc
actttgaacgaaccaactaaaaaatggctggaagagaagattgttgagcaggtatcggct
cgtgtacatcaactggaagcaatgatggctgaaaaagcgacatcggcaagaaatgagatg
gaaaagatgttgagagctcaaatcgaagcagaaatggcagtagaattggcggaatgcaag
aaacgggatgaagaatctcgtaaaaagtgcaaacaattggaagcagaactagaaagaaaa
gttctggaagctgaagaaagtcgcaagaaatttgaggaagatcgtttggcaatgcttgag
caaaagagccaattggaacgagatcgtgctgaacttgcacgtcagaaatcggacatgaaa
aagaatgagcagcaggcgatattgaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACGGAGGCATCGAAGCTCATCGT 20 125 133 Antisense
AAGCTCATCGTCAGAAGGTTCACAA 203 151 147 Antisense
TTCACAAGCCGAACCACATCAATTG 538 685 165 Antisense
CACAAGCATCCCATTCGACGTGAGC 158 195 288 Antisense
GTGAGCACTTTGAACGAACCAACTA 404 483 307 Antisense
GTTGAGCAGGTATCGGCTCGTGTAC 98 437 355 Antisense
ATCGGCTCGTGTACATCAACTGGAA 353 35 366 Antisense
GGAAGATCGTTTGGCAATGCTTGAG 327 531 588 Antisense
AGAGCCAATTGGAACGAGATCGTGC 105 71 617 Antisense
GATCGTGCTGAACTTGCACGTCAGA 187 419 634 Antisense
GAATGAGCAGCAGGCGATATTGAAC 561 331 675 Antisense
Sequence Source Affymetrix Proprietary Database