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[188306_at]

Description Y105C5B.2 /REP_DB=WormBase Gene ID /WP=CE24069 /GEN=gcy-25 /TR=Q9U317 /GB=CAB60311.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 06      4grp_total > 03      6grp_cgc > 21      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 71.8
(-0.8)
A 		54.2
(-3)
A 		56.5
(-2.7)
A 		102.9
(3)
A 		49
B 72.4
(2.6)
A 		58.9
(-0.3)
A 		46.6
(-3)
A 		74.4
(3)
P 		28
C 50.3
(-2.9)
A 		48.5
(-3)
A 		61.6
(-2.4)
A 		175.1
(3)
A 		127
avr 64.8
(-2) 		53.9
(-3) 		54.9
(-2.9) 		117.5
(3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 99
(3)
M 		50.4
(-1.2)
A 		84.9
(1.8)
A 		47.5
(-1.4)
A 		42.8
(-1.8)
A 		29
(-3)
A 		70
B 78.4
(3)
A 		39.7
(-3)
P 		57.1
(-0.3)
A 		56.3
(-0.4)
A 		64
(0.8)
A 		44.4
(-2.3)
A 		39
C 75.3
(2.1)
A 		87.8
(3)
A 		33.8
(-0.9)
A 		3.7
(-3)
A 		22.1
(-1.7)
A 		40.1
(-0.4)
A 		84
avr 84.2
(3) 		59.3
(-0.1) 		58.6
(-0.2) 		35.8
(-3) 		43
(-2.1) 		37.8
(-2.8) 		48
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 26.1
(3)
A 		11.8
(-3)
A 		14.8
(-1.7)
A 		15.1
(-1.6)
A 		14
B 23.1
(3)
A 		14.6
(-1.1)
A 		10.8
(-3)
A 		15.9
(-0.5)
P 		12
C 16.7
(-1.9)
A 		13.9
(-3)
A 		21.5
(-0.1)
A 		29.5
(3)
A 		16
avr 22
(3) 		13.4
(-3) 		15.7
(-1.4) 		20.2
(1.7) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 18.3
(2.9)
M 		17.9
(2.7)
A 		18.5
(3)
A 		12
(-0.4)
A 		12.9
(0.1)
A 		7.1
(-3)
A 		11
B 27.3
(3)
A 		13.2
(-3)
P 		13.9
(-2.7)
A 		14.9
(-2.3)
A 		26
(2.4)
A 		16.5
(-1.6)
A 		14
C 27.9
(3)
A 		26.8
(2.8)
A 		8.6
(-1.3)
A 		0.7
(-3)
A 		7.1
(-1.6)
A 		13.4
(-0.2)
A 		27
avr 24.5
(3) 		19.3
(1) 		13.7
(-1.2) 		9.2
(-3) 		15.3
(-0.6) 		12.3
(-1.8) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188306_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9275
Sequence Type Exemplar sequence
Representative Public ID Y105C5B.2 NCBI
Target Description Y105C5B.2 /REP_DB=WormBase Gene ID /WP=CE24069 /GEN=gcy-25 /TR=Q9U317 /GB=CAB60311.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070490(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070490 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-25) (gcy-25) mRNA, complete cds. 11/11 None
SNAP00000018386 ENSEMBL cdna:SNAP chromosome:CEL140:IV:15872922:15882339:1 10/11 None
GENEFINDER00000018422 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:15871293:15883457:1 11/11 None
Y105C5B.2 ENSEMBL cdna:known chromosome:CEL140:IV:15872922:15883457:1 gene:Y105C5B.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:15872927-15883463(+) 99.21 100.0
Public Domain and Genome References
Gene Title
Gene Symbol gcy-25
Ensembl Y105C5B.2
Entrez Gene 191653 Entrez gene
SwissProt Q9U317 EMBL-EBI
WormBase CE24069 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60311 Hypothetical protein Y105C5B.2 [Caenorhabditis elegans] ref|NP_502891.1| Guanylyl CYclase family member (gcy-25) [Caenorhabditis elegans] 0.0
blast T26377 hypothetical protein Y105C5B.a - Caenorhabditis elegans 0.0
blast CAE57395 Hypothetical protein CBG00346 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 9.9E-48
Pfam IPR001054 EMBL-EBI Guanylate cyclase 9.9E-48
Pfam IPR001054 EMBL-EBI Guanylate cyclase 9.9E-48
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502891.1 2 284-306,441-463
Y105C5B.2 2 284-306,441-463
SNAP00000018386 1 411-433
GENEFINDER00000018422 5 12-34,54-76,89-111,417-439,574-596
Sequence
Target Sequence 
>C. ELEGANS:188306_AT
aaactctacaagaactctactcctccttcgacaacattgtacagaaaaataaggcgttta
aagtggaaaacgtgggagatgcctacctgatttgcagtggaatacctgaaatgagtggat
ttcgtcatttaagagaaatttgcaaaatttcgctgaaattgcaagcgttcatgaaaacgt
tcaaagtccgtcatagaccatctcacacactacaaataaaaatgggaattaccagtggag
cagttgcagctggaatactgggaagtacggctccaagattttgtatattcggtgatactg
tgaatatggcgtgtcgtatggcaagtactggaaatcctggaagtattcaattaagtgaat
tgactgcaaatacgttgatggagaagtttccatcgtttatgttggaggagagaggcatga
ttgatgtgaagggaaaaggcgcttgcttaaccttctggttaactggagaaaaggacataa
tgagacgtcaatcttctcgttccagctgcataagtcagattaaattcgagttggatgaag
ctgacaattcgaagaaaatgtttctaaatccatacatcagtatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACTCTACAAGAACTCTACTCCTC 473 127 2675 Antisense
AAACGTGGGAGATGCCTACCTGATT 434 117 2742 Antisense
GATGCCTACCTGATTTGCAGTGGAA 6 415 2752 Antisense
CAAAGTCCGTCATAGACCATCTCAC 169 193 2856 Antisense
GACCATCTCACACACTACAAATAAA 605 383 2870 Antisense
GAATTACCAGTGGAGCAGTTGCAGC 352 323 2900 Antisense
GGAAGTACGGCTCCAAGATTTTGTA 404 529 2935 Antisense
GAATATGGCGTGTCGTATGGCAAGT 66 327 2976 Antisense
AAAAGGCGCTTGCTTAACCTTCTGG 479 127 3108 Antisense
TTCTCGTTCCAGCTGCATAAGTCAG 317 693 3168 Antisense
GTTTCTAAATCCATACATCAGTATC 656 449 3234 Antisense
Sequence Source Affymetrix Proprietary Database