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[188305_at]

Description Y55F3AL.1 /REP_DB=WormBase Gene ID /WP=CE25480 /GEN=plx-1 /TR=Q9N375 /GB=AAF60788.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 24      4grp_total > 37      6grp_cgc > 30      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 40.4
(1.5)
A 		51
(3)
A 		8.1
(-3)
A 		13.9
(-2.2)
A 		43
B 50.5
(3)
A 		15.3
(-3)
A 		28.6
(-0.7)
A 		33.5
(0.1)
A 		35
C 67.6
(3)
A 		46.4
(0.9)
A 		6
(-3)
A 		38.2
(0.1)
A 		62
avr 52.8
(3) 		37.6
(0.6) 		14.2
(-3) 		28.5
(-0.8) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 76.4
(3)
A 		39.3
(-0.4)
A 		33.5
(-1)
A 		23.1
(-1.9)
A 		11.6
(-3)
A 		14
(-2.8)
A 		65
B 21.4
(-1.9)
A 		54.8
(3)
A 		26.4
(-1.1)
A 		38.6
(0.6)
A 		13.6
(-3)
A 		13.5
(-3)
A 		41
C 33.4
(2.7)
A 		33.5
(2.8)
A 		34.5
(3)
A 		13.7
(-1.8)
A 		18.7
(-0.6)
A 		8.5
(-3)
A 		26
avr 43.7
(3) 		42.5
(2.8) 		31.5
(0.7) 		25.1
(-0.5) 		14.6
(-2.5) 		12
(-3) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 14.7
(3)
A 		11.1
(1.3)
A 		2.1
(-3)
A 		2
(-3)
A 		13
B 16.1
(3)
A 		3.8
(-3)
A 		6.6
(-1.6)
A 		7.1
(-1.4)
A 		12
C 22.4
(3)
A 		13.3
(0.3)
A 		2.1
(-3)
A 		6.4
(-1.7)
A 		20
avr 17.7
(3) 		9.4
(-0.5) 		3.6
(-3) 		5.2
(-2.3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 14.1
(3)
A 		14
(2.9)
A 		7.3
(-0.8)
A 		5.9
(-1.6)
A 		3.5
(-2.9)
A 		3.4
(-3)
A 		11
B 7.5
(-1.9)
A 		18.2
(3)
A 		6.4
(-2.4)
A 		10.2
(-0.6)
A 		5.5
(-2.8)
A 		5
(-3)
A 		13
C 12.4
(3)
A 		10.2
(1.6)
A 		8.8
(0.8)
A 		2.7
(-3)
A 		6.1
(-0.9)
A 		2.8
(-2.9)
A 		10
avr 11.3
(1.4) 		14.1
(3) 		7.5
(-0.8) 		6.3
(-1.5) 		5
(-2.3) 		3.7
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188305_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11207
Sequence Type Exemplar sequence
Representative Public ID Y55F3AL.1 NCBI
Target Description Y55F3AL.1 /REP_DB=WormBase Gene ID /WP=CE25480 /GEN=plx-1 /TR=Q9N375 /GB=AAF60788.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067617(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067617 NCBI Caenorhabditis elegans PLeXin family member (plx-1) (plx-1) mRNA, complete cds. 11/11 None
SNAP00000030046 ENSEMBL cdna:SNAP chromosome:CEL140:IV:980654:983709:1 11/11 None
GENEFINDER00000030071 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:981599:984310:1 11/11 None
Y55F3AL.1 ENSEMBL cdna:known chromosome:CEL140:IV:959452:984392:1 gene:Y55F3AL.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:959526-983149(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol plx-1
Ensembl Y55F3AL.1
Entrez Gene 246019 Entrez gene
WormBase CE37069 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.2962.1.S1_S_AT similar to plexin A4 cfa
DROSOPHILA_2:1640271_S_AT plexin A dm
DROSGENOME1:153857_AT plexin A dm
CHICKEN:GGAAFFX.4028.1.S1_AT similar to plexin-A4 gga
CHICKEN:GGAAFFX.4028.2.S1_AT similar to plexin-A4 gga
CHICKEN:GGAAFFX.4028.2.S1_S_AT similar to plexin-A4 gga
CHICKEN:GGAAFFX.4028.3.S1_S_AT similar to plexin-A4 gga
MOUSE430_2:1441371_AT plexin A4 mm
MOE430B:1441371_AT plexin A4 mm
MOUSE430_2:1457840_AT plexin A4 mm
MOE430B:1457840_AT plexin A4 mm
RAE230A:1376391_AT Plexin A4 (predicted) rn
RAT230_2:1376391_AT Plexin A4 (predicted) rn
RAE230B:1381249_AT Plexin A4 (predicted) rn
RAT230_2:1381249_AT Plexin A4 (predicted) rn
RAE230B:1381853_AT Plexin A4 (predicted) rn
RAT230_2:1381853_AT Plexin A4 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7275 development inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from direct assay QuickGO AmiGO
1726 ruffle inferred from direct assay QuickGO AmiGO
5884 actin filament inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
4872 receptor activity inferred from electronic annotation QuickGO AmiGO
17154 semaphorin receptor activity inferred from sequence similarity QuickGO AmiGO
48365 Rac GTPase binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast BAB85224.1 plexin A [Caenorhabditis elegans] 0.0
blast NP_500018 PLeXin family member (plx-1) [Caenorhabditis elegans] 0.0
blast ABA00169 Plexin protein 1 [Caenorhabditis elegans] 0.0
blast ABA00169 Plexin protein 1 [Caenorhabditis elegans] 1.0E-179
blast BAB85224.1 plexin A [Caenorhabditis elegans] 1.0E-172
Protein Domains
Database ID Description E-Value
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 5.1E-17
Pfam IPR002909 EMBL-EBI Cell surface receptor IPT/TIG 5.2E-14
Pfam IPR002909 EMBL-EBI Cell surface receptor IPT/TIG 1.2E-14
Pfam IPR002909 EMBL-EBI Cell surface receptor IPT/TIG 3.3E-7
Pfam IPR002165 EMBL-EBI Plexin 5.1E-12
Pfam IPR002165 EMBL-EBI Plexin 1.8E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500018.3 1 1258-1280
Y55F3AL.1 1 1258-1280
Sequence
Target Sequence 
>C. ELEGANS:188305_AT
gaaccctggcgaaattcatcacttctctgttggagagcatcttttcgtcgtctacagtac
ccccttgcattaagtatatgtttgattttatggatgaacaagctcgagagcatggaatca
acgatccggaggtggtgcacgcgtggaagagcaacgcattgcctctgaggttttgggtca
atttgatcaagaacccccatttcctattcgacatccaaaagcccaccaaaatcgaaggat
gcttgtcagtagttgctcaaactctaatggatgcctgttccactcaagaccatcagctca
cgaaagactcgccgagctcaaagctccttttcgcaaaggatatgtaccaatacagggacc
ttgtcgactcgtattacactgaaatttccatgacgccgcgtattgatgatgcgacgatgg
gcgccaatttgtctgcagaatcccgacaacacaacggagaattccacgttttttcggcgc
tcaacgagctcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACCCTGGCGAAATTCATCACTTC 88 347 5237 Antisense
TGTTGGAGAGCATCTTTTCGTCGTC 612 559 5264 Antisense
TACAGTACCCCCTTGCATTAAGTAT 322 643 5289 Antisense
TCAACGATCCGGAGGTGGTGCACGC 256 631 5354 Antisense
AGAGCAACGCATTGCCTCTGAGGTT 540 69 5384 Antisense
TCCTATTCGACATCCAAAAGCCCAC 337 591 5438 Antisense
GACCATCAGCTCACGAAAGACTCGC 567 383 5524 Antisense
AAAGACTCGCCGAGCTCAAAGCTCC 606 123 5539 Antisense
TACAGGGACCTTGTCGACTCGTATT 455 641 5587 Antisense
GGCGCCAATTTGTCTGCAGAATCCC 27 543 5656 Antisense
TTTTTTCGGCGCTCAACGAGCTCTA 557 675 5705 Antisense
Sequence Source Affymetrix Proprietary Database