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[188296_at]

Description F32A6.4 /REP_DB=WormBase Gene ID /WP=CE04472 /GEN=ags-3.1 /TR=Q19955 /GB=AAA81387.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 06      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 60      6grp_total > 07      4grp_total > 22      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 649
1 2 3 4 range
A 810.1
(2.8)
P 		358.3
(-2.1)
P 		275.2
(-3)
P 		828.5
(3)
P 		553
B 787.3
(2.2)
P 		416.4
(-1.6)
P 		283
(-3)
P 		861.2
(3)
P 		578
C 911.1
(3)
P 		397.1
(-1.8)
P 		261.9
(-3)
P 		739.3
(1.4)
P 		649
avr 836.2
(3) 		390.6
(-1.8) 		273.4
(-3) 		809.7
(2.7) 		563

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 359
1 2 3 4 5 6 range
A 501.6
(3)
P 		460.3
(2)
P 		322.2
(-1.5)
P 		370.1
(-0.3)
P 		260.7
(-3)
P 		360.9
(-0.5)
P 		241
B 475.9
(3)
P 		353.2
(-1.5)
P 		401.2
(0.3)
P 		352.1
(-1.5)
P 		330.3
(-2.3)
P 		312.6
(-3)
P 		163
C 619.7
(3)
P 		368.3
(-2.3)
P 		431.8
(-1)
P 		362.5
(-2.4)
P 		397.8
(-1.7)
P 		336.1
(-3)
P 		284
avr 532.4
(3) 		393.9
(-1.1) 		385.1
(-1.4) 		361.6
(-2.1) 		329.6
(-3) 		336.5
(-2.8) 		203
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 236
1 2 3 4 range
A 295
(3)
P 		77.8
(-2.8)
P 		72
(-3)
P 		121.3
(-1.7)
P 		223
B 251.1
(3)
P 		102.8
(-1.8)
P 		65.6
(-3)
P 		183.5
(0.8)
P 		186
C 301.6
(3)
P 		114.2
(-2.3)
P 		91.3
(-3)
P 		124.7
(-2)
P 		210
avr 282.6
(3) 		98.3
(-2.4) 		76.3
(-3) 		143.2
(-1.1) 		206

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 92.5
(-1.6)
P 		163.7
(3)
P 		70.2
(-3)
P 		93.9
(-1.5)
P 		78.7
(-2.5)
P 		87.9
(-1.9)
P 		94
B 165.6
(3)
P 		117.6
(-1)
P 		97.4
(-2.7)
P 		93.3
(-3)
P 		134.2
(0.4)
P 		116.6
(-1.1)
P 		72
C 229.7
(3)
P 		112.4
(-1.5)
P 		109.9
(-1.6)
P 		72.9
(-3)
P 		128.7
(-0.9)
P 		112.5
(-1.5)
P 		157
avr 162.6
(3) 		131.2
(0.5) 		92.5
(-2.5) 		86.7
(-3) 		113.9
(-0.8) 		105.7
(-1.5) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188296_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16048
Sequence Type Exemplar sequence
Representative Public ID F32A6.4 NCBI
Target Description F32A6.4 /REP_DB=WormBase Gene ID /WP=CE04472 /GEN=ags-3.1 /TR=Q19955 /GB=AAA81387.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171684(11), NM_171951(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171684 NCBI Caenorhabditis elegans Activator of G protein Signalling family member (ags-3) (ags-3) mRNA, complete cds. 11/11 None
NM_171951 NCBI Caenorhabditis elegans Activator of G protein Signalling family member (ags-3) (ags-3) mRNA, complete cds. 11/11 None
F32A6.4a ENSEMBL cdna:known chromosome:CEL140:X:5289688:5298005:-1 gene:F32A6.4 11/11 A 
F32A6.4b ENSEMBL cdna:known chromosome:CEL140:X:5290076:5297991:-1 gene:F32A6.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000030563 5/11 Cross Hyb Matching Probes None
GENEFINDER00000030572 5/11 Cross Hyb Matching Probes None
SNAP00000030564 5/11 Cross Hyb Matching Probes None
GENEFINDER00000030570 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180801 3 2 NM_171684 174401_at (11), 188296_at (11)
NM_171951 188296_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5290076-5297992(-) 100.0 100.0
Public Domain and Genome References
Ensembl F32A6.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81387 Activator of g protein signalling protein 3, isoform a [Caenorhabditis elegans] ref|NP_741787.1| Activator of G protein Signalling family member (ags-3) [Caenorhabditis elegans] 0.0
blast AAL27247 Activator of g protein signalling protein 3, isoform b [Caenorhabditis elegans] ref|NP_741788.1| Activator of G protein Signalling family member (ags-3) [Caenorhabditis elegans] 0.0
blast CAE68728 Hypothetical protein CBG14654 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003109 EMBL-EBI GoLoco 2.2E-12
Pfam IPR003109 EMBL-EBI GoLoco 2.6E-12
Pfam IPR003109 EMBL-EBI GoLoco 2.6E-12
Pfam IPR003109 EMBL-EBI GoLoco 2.4E-10
Pfam IPR001440 EMBL-EBI TPR repeat 9.1E-4
Pfam IPR001440 EMBL-EBI TPR repeat 0.096
Pfam IPR001440 EMBL-EBI TPR repeat 0.0076
Pfam IPR001440 EMBL-EBI TPR repeat 0.066
Pfam IPR001440 EMBL-EBI TPR repeat 3.3E-4
Pfam IPR003109 EMBL-EBI GoLoco 2.2E-12
Pfam IPR003109 EMBL-EBI GoLoco 2.6E-12
Pfam IPR003109 EMBL-EBI GoLoco 2.6E-12
Pfam IPR003109 EMBL-EBI GoLoco 2.4E-10
Pfam IPR001440 EMBL-EBI TPR repeat 0.0076
Pfam IPR001440 EMBL-EBI TPR repeat 0.066
Pfam IPR001440 EMBL-EBI TPR repeat 3.3E-4
Sequence
Target Sequence 
>C. ELEGANS:188296_AT
tgagcatcgcgaacttcgtcttacctactttcaaaaacgcaatattcacgataaaccagt
caatacgccggaggatccgctcgccgaggaccgctacgagaaggaagagttctttgatat
gcttgcaaagctgcagtcgaaacggatgaacgatcaacgtgtcgacgcgtcagttctcag
ttcaaatcttgactttcgagttcgccaagtggacactgagcccgacaactcatcgactga
tggttctgaggtactgatcgatttgcttctgaacgctcaaggacgaagaatggatgatca
gcgggcgccattcttgccggggctcaatccgaacggacagattattcttcgcagactcaa
tacagatcccggaaacgaggagctagatgaacatcttgtcgagtggctgatgcgtgttca
aggcgaacggcttgacgagcaacgtagcgagctgccacctatcaaaccagagggtcagga
agaagttgacaagaaaaaagaagaagatgtgaccgcaattgtgatgcgcatgcaagccgg
tcgcctcgaggaccaaagagcaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAGCATCGCGAACTTCGTCTTACC 546 569 1158 Antisense
CCAGTCAATACGCCGGAGGATCCGC 426 271 1213 Antisense
TCGCCGAGGACCGCTACGAGAAGGA 389 599 1238 Antisense
ACGATCAACGTGTCGACGCGTCAGT 701 91 1307 Antisense
AAATCTTGACTTTCGAGTTCGCCAA 217 117 1341 Antisense
GATCGATTTGCTTCTGAACGCTCAA 420 417 1413 Antisense
TCGCAGACTCAATACAGATCCCGGA 63 601 1506 Antisense
GTGGCTGATGCGTGTTCAAGGCGAA 601 491 1560 Antisense
TGACGAGCAACGTAGCGAGCTGCCA 268 571 1590 Antisense
ATTGTGATGCGCATGCAAGCCGGTC 362 3 1675 Antisense
GTCGCCTCGAGGACCAAAGAGCACA 369 475 1697 Antisense
Sequence Source Affymetrix Proprietary Database