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[188292_at] cuticulin

Description M142.2 /REP_DB=WormBase Gene ID /WP=CE06204 /TR=Q21540 /GB=CAA97806.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=cuticulin
Cluster 6grp_high1_10_10 > 92      6grp_total > 91      4grp_total > 26      6grp_cgc > 87      6grp_high1_sub > 04_05      6grp_cgc_sub > 05      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 8
(-3)
A 		37.9
(2.6)
A 		35.2
(2.1)
A 		39.9
(3)
A 		32
B 21.1
(-1.4)
A 		29
(-0.4)
A 		7.9
(-3)
A 		56.6
(3)
A 		49
C 8.5
(-3)
A 		39
(2.3)
A 		20.4
(-0.9)
A 		42.8
(3)
A 		34
avr 12.5
(-3) 		35.3
(1) 		21.2
(-1.5) 		46.4
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 957
1 2 3 4 5 6 range
A 56.3
(-2.7)
P 		14.4
(-3)
A 		84.8
(-2.6)
P 		971.3
(3)
P 		851.9
(2.3)
P 		106
(-2.4)
P 		957
B 34.2
(-3)
A 		93.5
(-2.6)
P 		141.9
(-2.3)
P 		930.1
(3)
P 		884.8
(2.7)
P 		144.2
(-2.3)
P 		896
C 55.9
(-3)
P 		49.9
(-3)
A 		59.8
(-2.9)
P 		880.8
(3)
P 		859.8
(2.8)
P 		130.9
(-2.4)
P 		831
avr 48.8
(-3) 		52.6
(-3) 		95.5
(-2.7) 		927.4
(3) 		865.5
(2.6) 		127
(-2.5) 		879
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 2.9
(-3)
A 		8.2
(2)
A 		9.2
(3)
A 		5.8
(-0.2)
A 		6
B 6.7
(-0.1)
A 		7.2
(0.1)
A 		1.8
(-3)
A 		12.1
(3)
A 		10
C 2.8
(-3)
A 		11.2
(3)
A 		7.1
(0.1)
A 		7.2
(0.1)
A 		8
avr 4.1
(-3) 		8.9
(3) 		6
(-0.6) 		8.4
(2.4) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 354
1 2 3 4 5 6 range
A 10.4
(-2.9)
P 		5.1
(-3)
A 		18.5
(-2.7)
P 		246.3
(2.7)
P 		257.1
(3)
P 		25.8
(-2.5)
P 		252
B 11.9
(-3)
A 		31.1
(-2.7)
P 		34.5
(-2.6)
P 		246.3
(1)
P 		359.5
(3)
P 		53.8
(-2.3)
P 		348
C 20.7
(-2.9)
P 		15.2
(-3)
A 		15.2
(-3)
P 		177.1
(0.7)
P 		278.2
(3)
P 		43.8
(-2.3)
P 		263
avr 14.3
(-3) 		17.1
(-2.9) 		22.7
(-2.8) 		223.2
(1.4) 		298.3
(3) 		41.1
(-2.4) 		284
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188292_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6919
Sequence Type Exemplar sequence
Representative Public ID M142.2 NCBI
Target Description M142.2 /REP_DB=WormBase Gene ID /WP=CE06204 /TR=Q21540 /GB=CAA97806.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=cuticulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066999(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066999 NCBI Caenorhabditis elegans M142.2 (cuticulin) mRNA, complete cds. 10/11 None
SNAP00000000571 ENSEMBL cdna:SNAP chromosome:CEL140:III:10907475:10916434:1 11/11 None
GENEFINDER00000000576 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10911376:10916444:1 10/11 None
M142.2 ENSEMBL cdna:known chromosome:CEL140:III:10911355:10916444:1 gene:M142.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10911371-10915333(+) 98.79 100.0
Public Domain and Genome References
Gene Title cuticulin
Gene Symbol cut-6
Ensembl M142.2
Entrez Gene 176520 Entrez gene
SwissProt Q21540 EMBL-EBI
WormBase CE35746 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA97806 Hypothetical protein M142.2 [Caenorhabditis elegans] ref|NP_499400.2| M142.2 [Caenorhabditis elegans] 0.0
blast CAE71373 Hypothetical protein CBG18277 [Caenorhabditis briggsae] 0.0
blast CAE71373 Hypothetical protein CBG18277 [Caenorhabditis briggsae] 1.0E-171
blast CAE71372 Hypothetical protein CBG18276 [Caenorhabditis briggsae] 1.0E-122
Protein Domains
Database ID Description E-Value
Pfam IPR002035 EMBL-EBI von Willebrand factor, type A 5.3E-58
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 7.4E-54
Pfam IPR008015 EMBL-EBI GMP-PDE, delta subunit 5.8E-78
Pfam IPR002035 EMBL-EBI von Willebrand factor, type A 5.3E-58
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 7.4E-54
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499400.2 1 542-564
M142.2 1 542-564
GENEFINDER00000000576 1 542-564
Sequence
Target Sequence 
>C. ELEGANS:188292_AT
acagtgttccgtgagcaaatctatcaaatgcctcaatgtgcctacaccctccgaaaaggc
gcccctgatggcccaatagtccgttttgcaactctcggtgaaagtgtatatcatagatgg
gaatgcattgaagtggagggtgctgataaggacactttcggaatgttagttcactcttgc
tatgtggataacggctacggtgatagagtggatattcttgattcgaatggttgtggccta
gacgcggtgcttctctcaactccggactacgatacttctctgcgtctcgccacgaaaccc
taccacgtatttaagtacgcggaccgcccggtactacaatttcagtgtcaaataacacta
tgtctgaaatatgatggaggatgtgaagggattactcctccacagaattgtaagaagctt
cctggtgaggatggtcatcatcatcatcatcatccggagaaaaggaggaaattggttagg
cgactggcggatggtgttggaactattgatgtatttaccgattctgttacggttctggaa
caggagccagcgtgtcagcaagtaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGTGTTCCGTGAGCAAATCTATC 552 107 1045 Antisense
TCAAATGCCTCAATGTGCCTACACC 408 629 1068 Antisense
CCCTGATGGCCCAATAGTCCGTTTT 349 259 1107 Antisense
TAGTCCGTTTTGCAACTCTCGGTGA 255 653 1121 Antisense
GAATGTTAGTTCACTCTTGCTATGT 377 331 1205 Antisense
CGAATGGTTGTGGCCTAGACGCGGT 439 253 1268 Antisense
TCAACTCCGGACTACGATACTTCTC 254 629 1300 Antisense
CCGCCCGGTACTACAATTTCAGTGT 695 261 1368 Antisense
GTGAAGGGATTACTCCTCCACAGAA 524 481 1427 Antisense
TAGGCGACTGGCGGATGGTGTTGGA 691 649 1521 Antisense
AGGAGCCAGCGTGTCAGCAAGTAAC 287 51 1586 Antisense
Sequence Source Affymetrix Proprietary Database