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[188290_at]

Description ZK75.3 /REP_DB=WormBase Gene ID /WP=CE02102 /GEN=ins-3 /TR=SW:Q09628 /GB=AAC46746.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 24      6grp_total > 15      4grp_total > 20      6grp_cgc > 42      6grp_high1_sub > 02      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 94.2
(-0.2)
P 		85.1
(-0.6)
A 		31.5
(-3)
A 		164.2
(3)
P 		133
B 83.5
(-2.4)
M 		87.7
(-1.9)
A 		77.9
(-3)
A 		133.8
(3)
P 		56
C 86.9
(0.6)
P 		73.4
(-0.3)
A 		34.6
(-3)
A 		122.2
(3)
P 		88
avr 88.2
(-0.4) 		82.1
(-0.8) 		48
(-3) 		140.1
(3) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 94
(2.1)
P 		103.2
(3)
P 		98.8
(2.6)
A 		62
(-1.1)
A 		42.2
(-3)
A 		58.6
(-1.4)
A 		61
B 106.3
(2.4)
P 		115.7
(3)
P 		80.2
(0.5)
P 		28.9
(-3)
A 		94.3
(1.5)
P 		81.9
(0.7)
P 		87
C 125.1
(2.4)
P 		133.2
(3)
P 		78.4
(-1.2)
A 		111.7
(1.3)
P 		99.4
(0.4)
P 		55.6
(-3)
A 		78
avr 108.5
(2) 		117.4
(3) 		85.8
(-0.6) 		67.5
(-2.8) 		78.6
(-1.5) 		65.4
(-3) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 34.3
(3)
P 		18.5
(-0.6)
A 		8.2
(-3)
A 		24
(0.6)
P 		26
B 26.6
(1.9)
M 		21.7
(-0.9)
A 		18
(-3)
A 		28.5
(3)
P 		11
C 28.8
(3)
P 		21.1
(0.2)
A 		12.1
(-3)
A 		20.6
(0.1)
P 		17
avr 29.9
(3) 		20.4
(-0.3) 		12.8
(-3) 		24.4
(1.1) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 17.3
(-1.9)
P 		36.7
(3)
P 		21.5
(-0.8)
A 		15.7
(-2.3)
A 		12.7
(-3)
A 		14.3
(-2.6)
A 		24
B 37
(2.7)
P 		38.5
(3)
P 		19.5
(-0.7)
P 		7.7
(-3)
A 		38.3
(3)
P 		30.5
(1.4)
P 		31
C 46.4
(3)
P 		40.6
(1.7)
P 		20
(-2.7)
A 		22.5
(-2.2)
P 		32.2
(-0.1)
P 		18.6
(-3)
A 		28
avr 33.6
(1.7) 		38.6
(3) 		20.3
(-1.7) 		15.3
(-3) 		27.7
(0.2) 		21.1
(-1.5) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188290_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4599
Sequence Type Exemplar sequence
Representative Public ID ZK75.3 NCBI
Target Description ZK75.3 /REP_DB=WormBase Gene ID /WP=CE02102 /GEN=ins-3 /TR=SW:Q09628 /GB=AAC46746.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062794(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062794 NCBI Caenorhabditis elegans INSulin related family member (ins-3) (ins-3) mRNA, complete cds. 11/11 None
SNAP00000034977 ENSEMBL cdna:SNAP chromosome:CEL140:II:5990407:5990670:-1 9/11 None
ZK75.3 ENSEMBL cdna:known chromosome:CEL140:II:5990266:5990670:-1 gene:ZK75.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5990262-5990667(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-3
Ensembl ZK75.3
Entrez Gene 191684 Entrez gene
SwissProt Q09628 EMBL-EBI
WormBase CE02102 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7582 physiological process inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46746 Insulin related protein 3 [Caenorhabditis elegans] ref|NP_495195.1| INSulin related family member (ins-3) [Caenorhabditis elegans] sp|Q09628|ILB3_CAEEL Probable insulin-like peptide beta-type 3 precursor 3.0E-58
blast AAC46746 Insulin related protein 3 [Caenorhabditis elegans] ref|NP_495195.1| INSulin related family member (ins-3) [Caenorhabditis elegans] sp|Q09628|ILB3_CAEEL Probable insulin-like peptide beta-type 3 precursor 1.0E-39
blast CAE59232 Hypothetical protein CBG02553 [Caenorhabditis briggsae] 2.0E-30
blast CAE59232 Hypothetical protein CBG02553 [Caenorhabditis briggsae] 8.0E-19
Protein Domains
Database ID Description E-Value
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 4.2E-32
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 2.1E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495195.1 1 4-26
ZK75.3 1 4-26
SNAP00000034977 1 4-26
Sequence
Target Sequence 
>C. ELEGANS:188290_AT
cgttgttcttgcacttttcattattttccaacttggagctgcaagtcttatgcgtaactg
gatgttcgattttgagaaagaattggaacacgattatgatgattcggaaattggattcca
taacattcactccctgatggccagatcaagaagaggagacaaagtgaagatttgtggtac
aaaagttctgaaaatggtgatggtaatgtgtggaggagaatgttcatcaacgaatgagaa
catcgctacagaatgctgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTTGTTCTTGCACTTTTCATTATT 93 247 24 Antisense
TGTTCTTGCACTTTTCATTATTTTC 154 563 27 Antisense
ATTATTTTCCAACTTGGAGCTGCAA 616 13 43 Antisense
GGAGCTGCAAGTCTTATGCGTAACT 699 521 58 Antisense
GTCTTATGCGTAACTGGATGTTCGA 216 469 68 Antisense
ACACGATTATGATGATTCGGAAATT 551 109 111 Antisense
TGGATTCCATAACATTCACTCCCTG 519 549 135 Antisense
TCACTCCCTGATGGCCAGATCAAGA 148 627 150 Antisense
AATGGTGATGGTAATGTGTGGAGGA 321 163 216 Antisense
GTGTGGAGGAGAATGTTCATCAACG 3 487 231 Antisense
GAGAACATCGCTACAGAATGCTGTG 686 391 259 Antisense
Sequence Source Affymetrix Proprietary Database