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[188287_at] 7TM receptor, srb family

Description R05H5.6 /REP_DB=WormBase Gene ID /WP=CE02293 /GEN=srb-6 /TR=SW:P54141 /GB=CAA88729.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 91      4grp_total > 0      6grp_cgc > 96      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 14.4
(-1.4)
A 		6.8
(-3)
A 		36.1
(3)
A 		32.4
(2.2)
A 		29
B 25.9
(-0.1)
P 		10.6
(-3)
A 		21.2
(-1)
A 		41.9
(3)
P 		31
C 28.9
(-2.2)
M 		18.9
(-3)
A 		21.3
(-2.8)
A 		95.3
(3)
P 		76
avr 23.1
(-1.5) 		12.1
(-3) 		26.2
(-1.1) 		56.5
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 39.3
(2.1)
A 		2.9
(-3)
A 		26.1
(0.2)
M 		45.8
(3)
A 		44.6
(2.8)
A 		4.8
(-2.7)
A 		43
B 22.1
(-0.9)
A 		19.6
(-1.4)
A 		38.9
(2.7)
M 		12.1
(-3)
A 		40.3
(3)
P 		25.9
(-0.1)
P 		28
C 26.2
(-0.2)
A 		35
(0.8)
A 		2.1
(-3)
A 		54.5
(3)
A 		5.3
(-2.6)
A 		11.4
(-1.9)
A 		52
avr 29.2
(0.9) 		19.2
(-1.7) 		22.4
(-0.9) 		37.5
(3) 		30.1
(1.1) 		14
(-3) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 5.3
(-0.1)
A 		1.5
(-3)
A 		9.4
(3)
A 		4.7
(-0.6)
A 		8
B 8.3
(2.4)
P 		2.6
(-3)
A 		4.9
(-0.8)
A 		8.9
(3)
P 		6
C 9.6
(-0.6)
M 		5.4
(-3)
A 		7.4
(-1.9)
A 		16.1
(3)
P 		11
avr 7.7
(1) 		3.2
(-3) 		7.2
(0.6) 		9.9
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 7.2
(-0)
A 		1
(-3)
A 		5.7
(-0.7)
M 		11.6
(2.1)
A 		13.5
(3)
A 		1.2
(-2.9)
A 		13
B 7.7
(-1)
A 		6.5
(-1.5)
A 		9.5
(-0.1)
M 		3.2
(-3)
A 		16.4
(3)
P 		9.7
(-0)
P 		13
C 9.7
(2.3)
A 		10.7
(2.8)
A 		0.5
(-3)
A 		11
(3)
A 		1.7
(-2.3)
A 		3.8
(-1.1)
A 		11
avr 8.2
(0.5) 		6.1
(-1.7) 		5.2
(-2.7) 		8.6
(1) 		10.5
(3) 		4.9
(-3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188287_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2702
Sequence Type Exemplar sequence
Representative Public ID R05H5.6 NCBI
Target Description R05H5.6 /REP_DB=WormBase Gene ID /WP=CE02293 /GEN=srb-6 /TR=SW:P54141 /GB=CAA88729.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063798(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063798 NCBI Caenorhabditis elegans Serpentine Receptor, class B (beta) family member (srb-6) (srb-6) mRNA, complete cds. 11/11 None
SNAP00000021967 ENSEMBL cdna:SNAP chromosome:CEL140:II:10195848:10196746:1 11/11 None
GENEFINDER00000021972 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10192440:10197241:1 11/11 None
R05H5.6 ENSEMBL cdna:known chromosome:CEL140:II:10194816:10196746:1 gene:R05H5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10194812-10196743(+) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM receptor, srb family
Gene Symbol srb-6
Ensembl R05H5.6
Entrez Gene 191788 Entrez gene
SwissProt P54141 EMBL-EBI
WormBase CE02293 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7606 sensory perception of chemical stimulus inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88729 Hypothetical protein R05H5.6 [Caenorhabditis elegans] ref|NP_496199.1| Serpentine Receptor, class B (beta) family member (srb-6) [Caenorhabditis elegans] sp|P54141|SRB6_CAEEL Serpentine receptor class beta-6 (Protein srb-6) prf||2123261U chemosensory receptor 0.0
blast CAA88729 Hypothetical protein R05H5.6 [Caenorhabditis elegans] ref|NP_496199.1| Serpentine Receptor, class B (beta) family member (srb-6) [Caenorhabditis elegans] sp|P54141|SRB6_CAEEL Serpentine receptor class beta-6 (Protein srb-6) prf||2123261U chemosensory receptor 1.0E-126
blast CAA88730 Hypothetical protein R05H5.7 [Caenorhabditis elegans] ref|NP_496198.1| R05H5.7 [Caenorhabditis elegans] 1.0E-112
blast CAE57856 Hypothetical protein CBG00894 [Caenorhabditis briggsae] 3.0E-77
blast CAE59057 Hypothetical protein CBG02340 [Caenorhabditis briggsae] 9.0E-55
blast NP_494963 Serpentine Receptor, class B (beta) family member (srb-3) [Caenorhabditis elegans] gb|AAK68209.1| Serpentine receptor, class b (beta) protein 3 [Caenorhabditis elegans] sp|Q95ZY2|SRB3_CAEEL Serpentine receptor class beta-3 (Protein srb-3) prf||2123261Q chemosensory receptor 5.0E-51
Protein Domains
Database ID Description E-Value
Pfam IPR002184 EMBL-EBI Integral membrane protein Srb, nematode type 2.3E-50
Pfam IPR002184 EMBL-EBI Integral membrane protein Srb, nematode type 1.2E-31
Pfam IPR002184 EMBL-EBI Integral membrane protein Srb, nematode type 2.3E-50
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496199.1 7 18-40,53-75,102-124,137-159,181-203,232-254,274-296
R05H5.6 7 18-40,53-75,102-124,137-159,181-203,232-254,274-296
SNAP00000021967 4 26-48,71-93,122-144,164-186
GENEFINDER00000021972 7 241-263,276-298,325-347,360-382,404-426,455-477,497-519
Sequence
Target Sequence 
>C. ELEGANS:188287_AT
ggagtgtttatcggtgtttttgcaatgctttgcgacatggcacttgtctatttcttcttc
ctcgatgagaaattcgatcaaccgtccatctcatactttatggttcctgatacttccggt
tataaaatgaattggttatgctattccttacttgctattaatagtgtaaatttagttttc
aattattttttggtaaagatcaacactattttgaaagaaaaatggcgtaattctttatca
actcgttatcaaatggaagagaacattataaccacgaaattctccacttttatctcattt
attcatgtattcttcttctcgctctatctcattttcaccctaattattcgtcttctagga
ccaggatttttgaaaacacaagccgatttgatgtcggtcagaggtgtttacatcactatt
cccacgtataatttgattattggtattgcttcatgcgtcattctacggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGTGTTTATCGGTGTTTTTGCAA 429 515 427 Antisense
ACATGGCACTTGTCTATTTCTTCTT 493 107 461 Antisense
GAGAAATTCGATCAACCGTCCATCT 101 391 493 Antisense
TGGTTCCTGATACTTCCGGTTATAA 282 549 527 Antisense
TTATGCTATTCCTTACTTGCTATTA 208 679 562 Antisense
GGCGTAATTCTTTATCAACTCGTTA 277 541 650 Antisense
ATTCTCCACTTTTATCTCATTTATT 320 11 705 Antisense
TTTATTCATGTATTCTTCTTCTCGC 57 675 724 Antisense
TTTCACCCTAATTATTCGTCTTCTA 238 673 759 Antisense
GGTGTTTACATCACTATTCCCACGT 544 499 829 Antisense
TTGCTTCATGCGTCATTCTACGGCA 268 703 872 Antisense
Sequence Source Affymetrix Proprietary Database