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[188282_at] heat shock protein

Description Y46H3A.3 /REP_DB=WormBase Gene ID /WP=CE22002 /GEN=hsp-16.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 86      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 59      6grp_total > 76      4grp_total > 23      6grp_highest_sub > In      6grp_cgc > 48      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4891
1 2 3 4 range
A 5481.7
(3)
P 		590.4
(-3)
P 		1755.2
(-1.6)
P 		3974.2
(1.2)
P 		4891
B 5354.1
(3)
P 		630.9
(-3)
P 		1773.4
(-1.5)
P 		3795.4
(1)
P 		4723
C 4970.2
(3)
P 		711.3
(-3)
P 		1877.3
(-1.4)
P 		3897.6
(1.5)
P 		4259
avr 5268.7
(3) 		644.2
(-3) 		1802
(-1.5) 		3889.1
(1.2) 		4625

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4247
1 2 3 4 5 6 range
A 1538.9
(-3)
P 		1521.3
(-3)
P 		2473.2
(-1.6)
P 		2877.6
(-1.1)
P 		2132.1
(-2.1)
P 		5736.6
(3)
P 		4215
B 1489.2
(-3)
P 		1562.5
(-2.9)
P 		2119.8
(-2)
P 		2319.6
(-1.7)
P 		2257.7
(-1.8)
P 		5369
(3)
P 		3880
C 1621
(-3)
P 		1647.2
(-3)
P 		2572.9
(-1.6)
P 		2711.3
(-1.4)
P 		2290.4
(-2)
P 		5684.9
(3)
P 		4064
avr 1549.7
(-3) 		1577
(-3) 		2388.6
(-1.8) 		2636.2
(-1.4) 		2226.7
(-2) 		5596.8
(3) 		4047
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1868
1 2 3 4 range
A 1995.9
(3)
P 		128.2
(-3)
P 		458.8
(-1.9)
P 		581.7
(-1.5)
P 		1868
B 1707.4
(3)
P 		155.8
(-3)
P 		410.9
(-2)
P 		808.8
(-0.5)
P 		1552
C 1645.3
(3)
P 		204.5
(-3)
P 		654.3
(-1.1)
P 		657.2
(-1.1)
P 		1441
avr 1782.9
(3) 		162.8
(-3) 		508
(-1.7) 		682.6
(-1.1) 		1620

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1718
1 2 3 4 5 6 range
A 283.7
(-3)
P 		541
(-1.6)
P 		538.6
(-1.6)
P 		729.8
(-0.6)
P 		643.4
(-1.1)
P 		1396.7
(3)
P 		1113
B 518.2
(-3)
P 		520.1
(-3)
P 		514.7
(-3)
P 		614.4
(-2.6)
P 		917.3
(-1.4)
P 		2002.1
(3)
P 		1487
C 600.8
(-2.6)
P 		502.6
(-3)
P 		654.8
(-2.3)
P 		545.3
(-2.8)
P 		741.1
(-2)
P 		1903.1
(3)
P 		1401
avr 467.6
(-3) 		521.2
(-2.8) 		569.4
(-2.5) 		629.8
(-2.3) 		767.3
(-1.6) 		1767.3
(3) 		1300
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188282_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15865
Sequence Type Exemplar sequence
Representative Public ID Y46H3A.3 NCBI
Target Description Y46H3A.3 /REP_DB=WormBase Gene ID /WP=CE22002 /GEN=hsp-16.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071106(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071106 NCBI Caenorhabditis elegans Heat Shock Protein family member (hsp-16.2) (hsp-16.2) mRNA, complete cds. 11/11 None
SNAP00000020847 ENSEMBL cdna:SNAP chromosome:CEL140:V:1804269:1804752:-1 11/11 None
GENEFINDER00000020855 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1804269:1804752:-1 11/11 None
Y46H3A.3 ENSEMBL cdna:known chromosome:CEL140:V:1804159:1804752:-1 gene:Y46H3A.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1804268-1804752(-) 100.0 100.0
Public Domain and Genome References
Gene Title heat shock protein
Gene Symbol hsp-16.2
Ensembl Y46H3A.3
Entrez Gene 178659 Entrez gene
SwissProt P06582 EMBL-EBI
WormBase CE22002 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503507 Heat Shock Protein family member (hsp-16.2) [Caenorhabditis elegans] gb|AAF60615.1| Heat shock protein protein 16.2 [Caenorhabditis elegans] sp|P06582|HSP12_CAEEL Heat shock protein Hsp-16.2 gb|AAA28071.1| heat shock protein 16-2 5.0E-76
blast AAB04839 Heat shock protein protein 16.1 [Caenorhabditis elegans] gb|AAB04842.1| Heat shock protein protein 16.11 [Caenorhabditis elegans] ref|NP_505357.1| Heat Shock Protein family member (hsp-16.11) [Caenorhabditis elegans] ref|NP_505354.1| Heat Shock Protein family member (hsp-16.1) [Caenorhabditis elegans] gb|AAA28068.1| heat shock protein 16-1a [Caenorhabditis elegans] sp|P34696|HSP11_CAEEL Heat shock protein Hsp16-1 7.0E-71
blast AAA28066 heat shock protein 16-1 [Caenorhabditis elegans] 2.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 8.1E-36
Sequence
Target Sequence 
>C. ELEGANS:188282_AT
gatatggctctgatggaacgccaatttgctccagtctgcagaatctctccatctgagtct
tctgagattgttaacaatgatcaaaagtttgccatcaatctcaacgtctcacagtttaaa
ccagaagatttgaaaattaatttggatggtcgtacgctatcaatccaaggagaacaagaa
ttgaagactgatcatggatattcaaaaaaatcattttctcgtgttattcttctacctgaa
gatgtagatgttggtgcagttgcttcgaatctttcagaagatggtaaactttcgattgaa
gcgccaaagaaagaagcggttcaaggaagatcaattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATGGCTCTGATGGAACGCCAAT 665 425 73 Antisense
TGATGGAACGCCAATTTGCTCCAGT 517 565 83 Antisense
TTTGCTCCAGTCTGCAGAATCTCTC 149 669 97 Antisense
GCAGAATCTCTCCATCTGAGTCTTC 561 313 110 Antisense
AAGTTTGCCATCAATCTCAACGTCT 355 157 157 Antisense
CATCAATCTCAACGTCTCACAGTTT 183 213 165 Antisense
AACGTCTCACAGTTTAAACCAGAAG 21 139 175 Antisense
ATTAATTTGGATGGTCGTACGCTAT 536 11 208 Antisense
GGTCGTACGCTATCAATCCAAGGAG 9 503 220 Antisense
GGTAAACTTTCGATTGAAGCGCCAA 212 505 355 Antisense
GAAGCGGTTCAAGGAAGATCAATTC 103 343 385 Antisense
Sequence Source Affymetrix Proprietary Database