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[188274_at]

Description K03B4.3 /REP_DB=WormBase Gene ID /WP=CE07323 /GEN=taf-11 /TR=Q21172 /GB=AAA97996.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 85      6grp_total > 59      4grp_total > 21      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 606
1 2 3 4 range
A 928.3
(0.3)
P 		657.1
(-2.4)
P 		597.3
(-3)
P 		1203.6
(3)
P 		606
B 989.7
(1)
P 		838.8
(-0.7)
P 		636.1
(-3)
P 		1163.7
(3)
P 		528
C 1017
(3)
P 		850.2
(0.6)
P 		607
(-3)
P 		962.7
(2.2)
P 		410
avr 978.3
(1.4) 		782
(-1) 		613.5
(-3) 		1110
(3) 		497

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 660
1 2 3 4 5 6 range
A 875.3
(1.1)
P 		570.5
(-1.9)
P 		459.5
(-3)
P 		629.8
(-1.3)
P 		925.4
(1.6)
P 		1062.2
(3)
P 		603
B 1000.3
(2.3)
P 		647.9
(-2.6)
P 		616.5
(-3)
P 		685.7
(-2)
P 		885.4
(0.7)
P 		1047.1
(3)
P 		431
C 667.4
(-1.8)
P 		577.9
(-2.8)
P 		557.9
(-3)
P 		684.8
(-1.6)
P 		664.8
(-1.9)
P 		1119.1
(3)
P 		561
avr 847.7
(0.4) 		598.8
(-2.4) 		544.6
(-3) 		666.8
(-1.6) 		825.2
(0.2) 		1076.1
(3) 		532
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 338
(3)
P 		142.6
(-3)
P 		156.1
(-2.6)
P 		176.2
(-2)
P 		195
B 315.6
(3)
P 		207.2
(-0.9)
P 		147.4
(-3)
P 		248
(0.6)
P 		168
C 336.6
(3)
P 		244.4
(-0.2)
P 		211.6
(-1.3)
P 		162.3
(-3)
P 		174
avr 330.1
(3) 		198.1
(-2) 		171.7
(-3) 		195.5
(-2.1) 		158

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 290
1 2 3 4 5 6 range
A 161.4
(-0.9)
P 		202.9
(0.4)
P 		100.1
(-3)
P 		159.7
(-1)
P 		279.2
(3)
P 		258.6
(2.3)
P 		179
B 348.1
(1.9)
P 		215.7
(-1.4)
P 		149.7
(-3)
P 		181.6
(-2.2)
P 		359.7
(2.2)
P 		390.5
(3)
P 		241
C 247.4
(-0.2)
P 		176.3
(-2)
P 		142
(-2.9)
P 		137.7
(-3)
P 		215.1
(-1)
P 		374.6
(3)
P 		237
avr 252.3
(0.5) 		198.3
(-1.1) 		130.6
(-3) 		159.7
(-2.2) 		284.7
(1.4) 		341.2
(3) 		211
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188274_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15520
Sequence Type Exemplar sequence
Representative Public ID K03B4.3 NCBI
Target Description K03B4.3 /REP_DB=WormBase Gene ID /WP=CE07323 /GEN=taf-11 /TR=Q21172 /GB=AAA97996.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071860(11), NM_071859(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071860 NCBI Caenorhabditis elegans TAF (TBP-associated transcription factor) family member (taf-10) (taf-10) mRNA, complete cds. 11/11 None
NM_071859 NCBI Caenorhabditis elegans TAF (TBP-associated transcription factor) family member (taf-10) (taf-10) mRNA, complete cds. 11/11 None
SNAP00000000711 ENSEMBL cdna:SNAP chromosome:CEL140:V:4683742:4687629:1 11/11 None
GENEFINDER00000000723 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4686928:4687629:1 11/11 None
K03B4.3a ENSEMBL cdna:known chromosome:CEL140:V:4686923:4688604:1 gene:K03B4.3 11/11 None
K03B4.3b ENSEMBL cdna:known chromosome:CEL140:V:4686928:4687629:1 gene:K03B4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4686927-4687629(+) 100.0 100.0
Public Domain and Genome References
Ensembl K03B4.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK82906 Taf (tbp-associated transcription factor) family protein 10, isoform b [Caenorhabditis elegans] ref|NP_504260.1| TAF (TBP-associated transcription factor) family member (taf-10) [Caenorhabditis elegans] 1.0E-99
blast AAA97996 Taf (tbp-associated transcription factor) family protein 10, isoform a [Caenorhabditis elegans] ref|NP_504261.1| TAF (TBP-associated transcription factor) family member (taf-10) [Caenorhabditis elegans] 5.0E-96
blast AAA97996 Taf (tbp-associated transcription factor) family protein 10, isoform a [Caenorhabditis elegans] ref|NP_504261.1| TAF (TBP-associated transcription factor) family member (taf-10) [Caenorhabditis elegans] 1.0E-87
blast AAA97996 Taf (tbp-associated transcription factor) family protein 10, isoform a [Caenorhabditis elegans] ref|NP_504261.1| TAF (TBP-associated transcription factor) family member (taf-10) [Caenorhabditis elegans] 2.0E-87
blast AAK82906 Taf (tbp-associated transcription factor) family protein 10, isoform b [Caenorhabditis elegans] ref|NP_504260.1| TAF (TBP-associated transcription factor) family member (taf-10) [Caenorhabditis elegans] 3.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR003923 EMBL-EBI Transcription initiation factor TFIID, 23-30 kDa subunit 1.9E-33
Pfam IPR003923 EMBL-EBI Transcription initiation factor TFIID, 23-30 kDa subunit 1.9E-33
Pfam IPR003923 EMBL-EBI Transcription initiation factor TFIID, 23-30 kDa subunit 1.9E-33
Sequence
Target Sequence 
>C. ELEGANS:188274_AT
agagcaatatgagccttcttcgtcgacagaaagcgtcctgatgcctccacccgcacttcc
acagcggccagcagcagctccacaagtttactccactcttgagccatctgttcaaaattt
aagatcgtcgttgcacaagcctctcaccggaaatcagcaacaatttgtgcagaaaacgct
ggaaaatgttcagaaaaatccatcgcaagacgacacacacgagttcatcaatcagctcgc
cgactatccgccgacgattcccgattctgtaacattgcacttcttgaagtctgccggtgt
tgatggatcggatccgcgagtgactagaatgatctcgctggcggctcaaaaacatgtttc
ggatattattcttgacgcgatgacaagtgctcgtatgaaaggtctcggaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGCAATATGAGCCTTCTTCGTCG 551 69 24 Antisense
TCGACAGAAAGCGTCCTGATGCCTC 459 601 46 Antisense
CTCCACAAGTTTACTCCACTCTTGA 620 235 101 Antisense
TCCACTCTTGAGCCATCTGTTCAAA 313 589 115 Antisense
TAAGATCGTCGTTGCACAAGCCTCT 259 637 143 Antisense
GCAAGACGACACACACGAGTTCATC 360 321 228 Antisense
CGAGTTCATCAATCAGCTCGCCGAC 154 251 243 Antisense
GACGATTCCCGATTCTGTAACATTG 455 377 276 Antisense
GAAGTCTGCCGGTGTTGATGGATCG 369 335 309 Antisense
TAGAATGATCTCGCTGGCGGCTCAA 197 651 348 Antisense
GTGCTCGTATGAAAGGTCTCGGACA 195 479 410 Antisense
Sequence Source Affymetrix Proprietary Database