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[188273_at]

Description T28H11.4 /REP_DB=WormBase Gene ID /WP=CE14375 /GEN=pes-1 /TR=Q27253 /GB=AAB04600.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 58      4grp_total > 07      6grp_cgc > 07      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 30.8
(-2)
A 		28.5
(-3)
A 		29
(-2.8)
A 		41.8
(3)
A 		13
B 59.9
(3)
A 		9.6
(-2.8)
A 		7.6
(-3)
A 		53.4
(2.3)
A 		52
C 71.3
(3)
A 		6.5
(-3)
A 		59.9
(1.9)
A 		71.3
(3)
A 		65
avr 54
(2.8) 		14.9
(-3) 		32.2
(-0.4) 		55.5
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 74.2
(3)
A 		36.2
(-0.6)
A 		10.4
(-3)
A 		45.5
(0.3)
A 		16.8
(-2.4)
A 		21.9
(-1.9)
A 		64
B 43.1
(1.6)
A 		7.4
(-2.6)
A 		19.1
(-1.2)
A 		3.6
(-3)
A 		3.9
(-3)
A 		55.3
(3)
P 		52
C 26.6
(-1.2)
A 		12
(-2.8)
A 		63.6
(3)
A 		10.6
(-3)
A 		53.1
(1.8)
A 		49.6
(1.4)
M 		53
avr 48
(3) 		18.5
(-3) 		31
(-0.5) 		19.9
(-2.7) 		24.6
(-1.8) 		42.3
(1.8) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 11.2
(3)
A 		6.2
(-2.9)
A 		7.6
(-1.2)
A 		6.1
(-3)
A 		5
B 19.1
(3)
A 		2.4
(-2.8)
A 		1.8
(-3)
A 		11.4
(0.3)
A 		17
C 23.6
(3)
A 		1.9
(-3)
A 		20.9
(2.3)
A 		12
(-0.2)
A 		22
avr 18
(3) 		3.5
(-3) 		10.1
(-0.3) 		9.8
(-0.4) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 13.7
(3)
A 		12.9
(2.6)
A 		2.3
(-3)
A 		11.5
(1.8)
A 		5.1
(-1.5)
A 		5.3
(-1.4)
A 		11
B 15
(1.3)
A 		2.4
(-2.6)
A 		4.6
(-1.9)
A 		1
(-3)
A 		1.6
(-2.8)
A 		20.6
(3)
P 		20
C 9.9
(0.1)
A 		3.7
(-2.4)
A 		16.2
(2.6)
A 		2.1
(-3)
A 		17.2
(3)
A 		16.6
(2.8)
M 		15
avr 12.9
(2.2) 		6.3
(-2.1) 		7.7
(-1.2) 		4.9
(-3) 		8
(-1) 		14.2
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188273_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11199
Sequence Type Exemplar sequence
Representative Public ID T28H11.4 NCBI
Target Description T28H11.4 /REP_DB=WormBase Gene ID /WP=CE14375 /GEN=pes-1 /TR=Q27253 /GB=AAB04600.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028235(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028235 NCBI Caenorhabditis elegans Patterned Expression Site family member (pes-1) (pes-1) mRNA, complete cds. 11/11 None
GENEFINDER00000000758 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5004404:5005337:1 11/11 None
T28H11.4 ENSEMBL cdna:known chromosome:CEL140:IV:5002742:5005337:1 gene:T28H11.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000000748 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5002747-5005343(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pes-1
Ensembl T28H11.4
Entrez Gene 177267 Entrez gene
SwissProt Q27253 EMBL-EBI
WormBase CE14375 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB04600 Patterned expression site protein 1 [Caenorhabditis elegans] emb|CAA82226.1| PES-1A protein [Caenorhabditis elegans] emb|CAA82224.1| PES-1 [Caenorhabditis elegans] ref|NP_001023406.1| Patterned Expression Site family member (pes-1) [Caenorhabditis elegans] 1.0E-127
blast CAA82225 PES-1B protein [Caenorhabditis elegans] 1.0E-105
blast AAB04600 Patterned expression site protein 1 [Caenorhabditis elegans] emb|CAA82226.1| PES-1A protein [Caenorhabditis elegans] emb|CAA82224.1| PES-1 [Caenorhabditis elegans] ref|NP_001023406.1| Patterned Expression Site family member (pes-1) [Caenorhabditis elegans] 1.0E-105
blast CAE56896 Hypothetical protein CBG24736 [Caenorhabditis briggsae] 5.0E-36
blast CAE56896 Hypothetical protein CBG24736 [Caenorhabditis briggsae] 6.0E-36
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 1.0000000272452E-27
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 3.0000000817356E-27
Pfam IPR001766 EMBL-EBI Fork head transcription factor 8.8E-33
Pfam IPR001766 EMBL-EBI Fork head transcription factor 1.4E-31
Sequence
Target Sequence 
>C. ELEGANS:188273_AT
acccgctctgaatcagtcggtcaacaacagtctggtagaaattcaccagtctcaagctcc
accgaatcaccaaccaaaagaccaaaatactcgtacaatgcattgatcgccatggctatt
cagagcagtccattcaaaagtttgagagtctccgagatctacaaatatatttcttccaac
ttctcctactacaaaaaccagaagccacttcaatggcaaaactcggttcgccacaacttg
tctttgcataaagagttccgaaaggttcgaactttggatggaaaaggcagctactgggca
atgactgcggatttaggaactgatgtttatatcagcaacaactgcggaaagcttcgtcgc
caaaaaagcaaagtggccaagtttccaccaatgcaacaacattttccaatccctcagctt
ccaacccaaaacattcaccaactttgtatgcaaaatcctcaaattcttgctacattgctt
caaaatatgtatcttcaaaatatgcaaaacctccagaatatccccatggttcctggcttt
ccaataattccagttcccattaatcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCCGCTCTGAATCAGTCGGTCAAC 487 87 211 Antisense
TAGAAATTCACCAGTCTCAAGCTCC 218 653 246 Antisense
GTACAATGCATTGATCGCCATGGCT 76 461 303 Antisense
CGCCATGGCTATTCAGAGCAGTCCA 13 257 318 Antisense
GTTCGCCACAACTTGTCTTTGCATA 703 441 436 Antisense
AACAACTGCGGAAAGCTTCGTCGCC 437 135 547 Antisense
ATGCAACAACATTTTCCAATCCCTC 523 43 601 Antisense
CTCAAATTCTTGCTACATTGCTTCA 414 227 668 Antisense
TGCAAAACCTCCAGAATATCCCCAT 372 575 713 Antisense
GGTTCCTGGCTTTCCAATAATTCCA 221 507 738 Antisense
AATAATTCCAGTTCCCATTAATCCT 575 171 753 Antisense
Sequence Source Affymetrix Proprietary Database