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[188272_at] ArsA

Description ZK637.5 /REP_DB=WormBase Gene ID /WP=CE00436 /TR=SW:P30632 /GB=CAA77452.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=ArsA
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 22      4grp_high_8_8 > 07      6grp_highest_nonorm > 67      6grp_total > 70      4grp_total > 01      6grp_highest_sub > 03_04      6grp_cgc > 94      6grp_high1_sub > 03_04      6grp_cgc_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2735
1 2 3 4 range
A 1627.3
(-1.7)
P 		1140.3
(-3)
P 		1506
(-2)
P 		3426.9
(3)
P 		2287
B 1467.9
(-2.8)
P 		1375.2
(-3)
P 		1576.8
(-2.5)
P 		3875.3
(3)
P 		2500
C 1635.5
(-1.9)
P 		1168.3
(-3)
P 		1625.8
(-1.9)
P 		3736.3
(3)
P 		2568
avr 1576.9
(-2.1) 		1227.9
(-3) 		1569.5
(-2.2) 		3679.5
(3) 		2452

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 755
1 2 3 4 5 6 range
A 1622.6
(-0.9)
P 		1358.5
(-3)
P 		1861.4
(1)
P 		2113.8
(3)
P 		1506.4
(-1.8)
P 		1581.3
(-1.2)
P 		755
B 1579.2
(0.4)
P 		1630.9
(1.8)
P 		1460.4
(-3)
P 		1639.6
(2.1)
P 		1586.1
(0.6)
P 		1671.9
(3)
P 		212
C 1651.6
(0.2)
P 		1686.1
(0.8)
P 		1687.7
(0.8)
P 		1826.9
(3)
P 		1450.7
(-3)
P 		1640.5
(0)
P 		376
avr 1617.8
(-1.2) 		1558.5
(-2.2) 		1669.8
(-0.3) 		1860.1
(3) 		1514.4
(-3) 		1631.2
(-1) 		346
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 578
1 2 3 4 range
A 592.5
(3)
P 		247.5
(-3)
P 		393.7
(-0.5)
P 		501.6
(1.4)
P 		345
B 468.1
(-1.4)
P 		339.7
(-3)
P 		365.4
(-2.7)
P 		825.8
(3)
P 		486
C 541.4
(1.2)
P 		335.9
(-3)
P 		566.6
(1.7)
P 		630
(3)
P 		294
avr 534
(0.9) 		307.7
(-3) 		441.9
(-0.7) 		652.5
(3) 		345

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 345
1 2 3 4 5 6 range
A 299.1
(-3)
P 		483.1
(1.7)
P 		405.4
(-0.3)
P 		536.1
(3)
P 		454.6
(0.9)
P 		385
(-0.8)
P 		237
B 549.5
(1)
P 		542.9
(0.9)
P 		354.6
(-3)
P 		434.3
(-1.3)
P 		644.4
(3)
P 		623.5
(2.6)
P 		290
C 612.2
(3)
P 		514.5
(0.6)
P 		429.5
(-1.5)
P 		367.4
(-3)
P 		469.4
(-0.5)
P 		549.2
(1.5)
P 		245
avr 486.9
(1.3) 		513.5
(2.6) 		396.5
(-3) 		445.9
(-0.7) 		522.8
(3) 		519.2
(2.8) 		126
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188272_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7524
Sequence Type Exemplar sequence
Representative Public ID ZK637.5 NCBI
Target Description ZK637.5 /REP_DB=WormBase Gene ID /WP=CE00436 /TR=SW:P30632 /GB=CAA77452.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=ArsA
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066564(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066564 NCBI Caenorhabditis elegans ZK637.5 (ArsA) mRNA, complete cds. 11/11 None
SNAP00000013202 ENSEMBL cdna:SNAP chromosome:CEL140:III:8894991:8896403:1 11/11 None
GENEFINDER00000013239 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8894991:8896403:1 11/11 None
ZK637.5 ENSEMBL cdna:known chromosome:CEL140:III:8894988:8896547:1 gene:ZK637.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8894986-8896399(+) 100.0 100.0
Public Domain and Genome References
Gene Title ArsA
Gene Symbol tag-205
Ensembl ZK637.5
Entrez Gene 176254 Entrez gene
SwissProt P30632 EMBL-EBI
WormBase CE00436 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261627_AT anion-transporting ATPase, putative at
CANINE:1592283_AT similar to arsA arsenite transporter, ATP-binding, homolog 1 cfa
CANINE:1585122_AT similar to arsA arsenite transporter, ATP-binding, homolog 1 cfa
CANINE_2:CFAAFFX.26326.1.S1_S_AT similar to arsA arsenite transporter, ATP-binding, homolog 1 cfa
CANINE_2:CFAAFFX.5088.1.S1_S_AT similar to arsA arsenite transporter, ATP-binding, homolog 1 cfa
CANINE_2:CFAAFFX.5088.1.S1_X_AT similar to arsA arsenite transporter, ATP-binding, homolog 1 cfa
DROSOPHILA_2:1639416_AT dm
DROSGENOME1:146748_AT dm
HUGENEFL:U60276_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) hs
HG-U133A:202024_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) hs
HG-U133_PLUS_2:202024_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) hs
HG-FOCUS:202024_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) hs
HG-U133A_2:202024_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) hs
U133_X3P:G4757795_3P_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) hs
HG-U95AV2:40439_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) hs
MG-U74AV2:92428_AT arsA (bacterial) arsenite transporter, ATP-binding, homolog 1 mm
MG-U74CV2:169775_R_AT arsA (bacterial) arsenite transporter, ATP-binding, homolog 1 mm
MOE430A:1418292_AT arsA (bacterial) arsenite transporter, ATP-binding, homolog 1 mm
MOUSE430A_2:1418292_AT arsA (bacterial) arsenite transporter, ATP-binding, homolog 1 mm
MOUSE430_2:1418292_AT arsA (bacterial) arsenite transporter, ATP-binding, homolog 1 mm
MU19KSUBA:TC20682_AT ArsA (bacterial) arsenite transporter, ATP-binding, homolog 1, mRNA (cDNA clone MGC:28999 IMAGE:2616359) mm
MU19KSUBC:TC39336_AT ArsA (bacterial) arsenite transporter, ATP-binding, homolog 1, mRNA (cDNA clone MGC:28999 IMAGE:2616359) mm
RG-U34B:RC_AA997032_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (predicted) rn
RAE230A:1372142_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (predicted) rn
RAT230_2:1372142_AT arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (predicted) rn
YEAST_2:1778988_AT ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6820 anion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA77452 Hypothetical protein ZK637.5 [Caenorhabditis elegans] ref|NP_498965.1| ZK637.5 [Caenorhabditis elegans] sp|P30632|ARSA_CAEEL Putative arsenical pump-driving ATPase (Arsenite-translocating ATPase) (Arsenical resistance ATPase) (Arsenite-transporting ATPase) 1.0E-171
blast CAE57614 Hypothetical protein CBG00595 [Caenorhabditis briggsae] 1.0E-155
blast XP_867457 PREDICTED: similar to arsA arsenite transporter, ATP-binding, homolog 1 isoform 4 [Canis familiaris] 5.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR003348 EMBL-EBI Anion-transporting ATPase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188272_AT
tgatagactctcttgacttcgacgttgtggtctttgacaccgctccaactgggcacactc
ttcgtcttcttcaattcccaacacttttggaaaaagtatttacgaaaattctgtcacttc
aaggaatgtttggaccaatgatgaatcaattcggtggaatgtttggaatgggaggtggat
caatgaatgaaatgattgaaaagatgacaacgactctagaatctgtgaaaaagatgaacg
cacagtttaaggatcctaattgcaccacattcgtctgcgtctgcattgccgaattcctct
ctctttacgaaaccgaacgacttattcaggagttgtccaaacaaggaatcgatactcata
atattattgtgaatcaacttctctttccggataccgatgcaaatggaacagtttcatgca
gaaaatgtgcatcgagacaggcgattcagtcgaaatacttgacagatatcgatgagctct
acgaggatttccacgtcgtcaaacttccacttcttgaggcagaagtgcgtggagggcccg
caattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATAGACTCTCTTGACTTCGACGT 48 565 449 Antisense
CGACGTTGTGGTCTTTGACACCGCT 131 251 468 Antisense
CTTCTTCAATTCCCAACACTTTTGG 451 221 514 Antisense
GAACGCACAGTTTAAGGATCCTAAT 514 343 684 Antisense
CTAATTGCACCACATTCGTCTGCGT 219 227 704 Antisense
TCTCTCTTTACGAAACCGAACGACT 249 595 746 Antisense
AACTTCTCTTTCCGGATACCGATGC 95 139 824 Antisense
GATGAGCTCTACGAGGATTTCCACG 684 411 919 Antisense
GGATTTCCACGTCGTCAAACTTCCA 112 509 933 Antisense
GTCAAACTTCCACTTCTTGAGGCAG 102 465 946 Antisense
GAAGTGCGTGGAGGGCCCGCAATTC 244 335 970 Antisense
Sequence Source Affymetrix Proprietary Database