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[188271_at]

Description B0261.2 /REP_DB=WormBase Gene ID /WP=CE07705 /TR=O01438 /GB=AAB52354.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=kinase
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 26      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 60      6grp_total > 26      4grp_total > 47      6grp_highest_sub > 01_02      6grp_cgc > 14      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 417
1 2 3 4 range
A 575.7
(3)
P 		215.4
(-3)
P 		255.2
(-2.3)
P 		258.6
(-2.3)
P 		360
B 612.4
(3)
P 		239.3
(-3)
P 		272.7
(-2.5)
P 		333
(-1.5)
P 		373
C 535.2
(3)
P 		195.3
(-3)
P 		304.1
(-1.1)
P 		257.4
(-1.9)
P 		340
avr 574.4
(3) 		216.7
(-3) 		277.3
(-2) 		283
(-1.9) 		358

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 445
1 2 3 4 5 6 range
A 455.2
(1.7)
P 		515.8
(3)
P 		274.6
(-2.3)
P 		295
(-1.9)
P 		244.8
(-3)
P 		310.8
(-1.5)
P 		271
B 432.4
(3)
P 		431.7
(3)
P 		265.2
(-1.4)
P 		276.7
(-1.1)
P 		204.1
(-3)
P 		335.9
(0.5)
P 		228
C 648.6
(3)
P 		463.2
(0.2)
P 		390
(-0.9)
P 		247
(-3)
P 		278.6
(-2.5)
P 		257.6
(-2.8)
P 		402
avr 512.1
(3) 		470.2
(2.1) 		309.9
(-1.5) 		272.9
(-2.3) 		242.5
(-3) 		301.4
(-1.7) 		270
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 172
1 2 3 4 range
A 209.6
(3)
P 		46.7
(-2.7)
P 		66.7
(-2)
P 		37.9
(-3)
P 		172
B 195.3
(3)
P 		59.1
(-3)
P 		63.2
(-2.8)
P 		71
(-2.5)
P 		136
C 177.2
(3)
P 		56.2
(-2.4)
P 		106
(-0.2)
P 		43.4
(-3)
P 		134
avr 194
(3) 		54
(-2.9) 		78.6
(-1.8) 		50.8
(-3) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 191
1 2 3 4 5 6 range
A 83.9
(-1.8)
P 		183.4
(3)
P 		59.8
(-3)
P 		74.8
(-2.3)
P 		73.9
(-2.3)
P 		75.7
(-2.2)
P 		124
B 150.4
(3)
P 		143.7
(2.5)
P 		64.4
(-3)
P 		73.3
(-2.4)
P 		82.9
(-1.7)
P 		125.3
(1.2)
P 		86
C 240.4
(3)
P 		141.3
(-0.1)
P 		99.3
(-1.4)
P 		49.7
(-3)
P 		90.2
(-1.7)
P 		86.2
(-1.9)
P 		191
avr 158.2
(3) 		156.1
(2.9) 		74.5
(-2.4) 		65.9
(-3) 		82.3
(-1.9) 		95.7
(-1.1) 		92
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188271_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.726
Sequence Type Exemplar sequence
Representative Public ID B0261.2 NCBI
Target Description B0261.2 /REP_DB=WormBase Gene ID /WP=CE07705 /TR=O01438 /GB=AAB52354.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059151(11), NM_059148(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059151 NCBI Caenorhabditis elegans LEThal family member (let-363) (let-363) mRNA, complete cds. 11/11 None
NM_059148 NCBI Caenorhabditis elegans LEThal family member (let-363) (let-363) mRNA, complete cds. 11/11 None
SNAP00000040715 ENSEMBL cdna:SNAP chromosome:CEL140:I:5243502:5262993:1 11/11 None
GENEFINDER00000040723 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5245814:5268119:1 11/11 None
B0261.2b ENSEMBL cdna:known chromosome:CEL140:I:5245746:5263427:1 gene:B0261.2 11/11 A 
B0261.2a ENSEMBL cdna:known chromosome:CEL140:I:5245784:5262993:1 gene:B0261.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5245783-5262993(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0261.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN84886 Lethal protein 363, isoform b [Caenorhabditis elegans] ref|NP_491552.2| LEThal family member (let-363) [Caenorhabditis elegans] 0.0
blast AAN84885 Lethal protein 363, isoform a [Caenorhabditis elegans] ref|NP_491549.2| LEThal family member (let-363) [Caenorhabditis elegans] 0.0
blast Q95Q95 Target of rapamycin homolog (CeTOR) (Lethal protein 363) pir||T25444 hypothetical protein B0261.2 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 1.1E-104
Pfam IPR010729 EMBL-EBI Mitochondrial 39-S ribosomal L47 9.6E-64
Pfam IPR003152 EMBL-EBI PIK-related kinase, FATC 3.8E-21
Pfam IPR003151 EMBL-EBI PIK-related kinase, FAT 1.0E-126
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 2.5E-4
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 1.1E-104
Pfam IPR003152 EMBL-EBI PIK-related kinase, FATC 4.6E-16
Pfam IPR003151 EMBL-EBI PIK-related kinase, FAT 1.0E-126
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 1.1E-104
Pfam IPR003152 EMBL-EBI PIK-related kinase, FATC 3.8E-21
Pfam IPR003151 EMBL-EBI PIK-related kinase, FAT 1.0E-126
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 1.1E-104
Pfam IPR003152 EMBL-EBI PIK-related kinase, FATC 3.8E-21
Pfam IPR003151 EMBL-EBI PIK-related kinase, FAT 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188271_AT
ggttggatatattcttggtctcggtgacagacatccatccaacttgatgctcgatcgatt
gaccggaaaagttgtacacattgatttcggtgactgtttcgaagttgctatgctccgtga
aaaattccctgaacgagttccattccgacttaccagaatgctcatcaacgctatggaagt
aactggattggacggagtatacaattacactgccgaacgtgtcttgaaaatgttgcgaac
aaatcaagaatcattgttagcagttttagaagcatttgtctatgatccagtaattaattg
gcgtcttgtggaaggaatgaaaaaggatccgaaaactagaaaagatacaggtggacgtca
gaatatggctggtgctgtattaccatcatcaagtacaactgattcaattatggagacgat
taagaggaaactcgatgggaccgaattcgttcacactgacggatcaactccacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTGGATATATTCTTGGTCTCGGT 131 509 7530 Antisense
TGGTCTCGGTGACAGACATCCATCC 227 553 7545 Antisense
GACATCCATCCAACTTGATGCTCGA 89 371 7559 Antisense
TGATGCTCGATCGATTGACCGGAAA 391 565 7574 Antisense
GTTTCGAAGTTGCTATGCTCCGTGA 294 449 7625 Antisense
TTCCCTGAACGAGTTCCATTCCGAC 95 697 7654 Antisense
TCCGACTTACCAGAATGCTCATCAA 616 595 7673 Antisense
ATTACACTGCCGAACGTGTCTTGAA 254 13 7733 Antisense
GGCTGGTGCTGTATTACCATCATCA 187 541 7896 Antisense
GATGGGACCGAATTCGTTCACACTG 462 407 7963 Antisense
TTCACACTGACGGATCAACTCCACC 127 685 7979 Antisense
Sequence Source Affymetrix Proprietary Database