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[188257_at]

Description T15H9.1 /REP_DB=WormBase Gene ID /WP=CE01664 /TR=SW:Q10005 /GB=CAA87414.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=DNAJ
Cluster 6grp_high1_10_10 > 48      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_total > 48      4grp_total > 01      6grp_cgc > 07      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 281
1 2 3 4 range
A 174.7
(-1.2)
P 		92.8
(-3)
P 		149.9
(-1.8)
P 		370.2
(3)
P 		277
B 164.4
(-2.3)
P 		137.8
(-3)
P 		143.9
(-2.8)
P 		373.3
(3)
P 		236
C 157.9
(-1.9)
P 		120
(-3)
P 		160.2
(-1.9)
P 		333.4
(3)
P 		213
avr 165.7
(-1.8) 		116.9
(-3) 		151.3
(-2.1) 		359
(3) 		242

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 70.1
(-1.9)
P 		71.5
(-1.7)
P 		103.9
(1)
P 		56.4
(-3)
P 		73.3
(-1.6)
P 		128.2
(3)
P 		72
B 165.2
(3)
P 		68
(-2.9)
P 		66.4
(-3)
P 		81
(-2.1)
A 		118.3
(0.2)
P 		113.7
(-0.1)
P 		99
C 177.7
(3)
P 		92.7
(-1)
P 		93.3
(-1)
P 		80.7
(-1.6)
P 		50.5
(-3)
P 		129.4
(0.7)
P 		127
avr 137.7
(3) 		77.4
(-2.6) 		87.9
(-1.6) 		72.7
(-3) 		80.7
(-2.3) 		123.8
(1.7) 		65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 63.6
(3)
P 		20.2
(-3)
P 		39.2
(-0.4)
P 		54.2
(1.7)
P 		43
B 52.4
(-0.5)
P 		34
(-2.9)
P 		33.3
(-3)
P 		79.6
(3)
P 		46
C 52.3
(1.9)
P 		34.5
(-3)
P 		55.8
(2.9)
P 		56.2
(3)
P 		22
avr 56.1
(1.7) 		29.6
(-3) 		42.8
(-0.6) 		63.3
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 12.9
(-3)
P 		25.4
(1.1)
P 		22.6
(0.2)
P 		14.3
(-2.5)
P 		22.1
(0)
P 		31.2
(3)
P 		18
B 57.5
(3)
P 		22.6
(-2.1)
P 		16.1
(-3)
P 		21.5
(-2.2)
A 		48.1
(1.6)
P 		42.4
(0.8)
P 		41
C 65.9
(3)
P 		28.3
(-1.5)
P 		23.8
(-2.1)
P 		16.2
(-3)
P 		16.4
(-3)
P 		43.3
(0.3)
P 		50
avr 45.4
(3) 		25.4
(-1.3) 		20.8
(-2.3) 		17.3
(-3) 		28.9
(-0.5) 		39
(1.6) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188257_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3346
Sequence Type Exemplar sequence
Representative Public ID T15H9.1 NCBI
Target Description T15H9.1 /REP_DB=WormBase Gene ID /WP=CE01664 /TR=SW:Q10005 /GB=CAA87414.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=DNAJ
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063671(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063671 NCBI Caenorhabditis elegans T15H9.1 (T15H9.1) mRNA, complete cds. 10/11 None
GENEFINDER00000036803 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9618551:9624081:-1 11/11 None
T15H9.1.2 ENSEMBL cdna:known chromosome:CEL140:II:9618551:9623175:-1 gene:T15H9.1 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171030 3/11 Cross Hyb Matching Probes None
SNAP00000036799 7/11 Cross Hyb Matching Probes None
SNAP00000036801 3/11 Cross Hyb Matching Probes None
T15H9.1.1 7/11 Cross Hyb Matching Probes A 
T15H9.7 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9618547-9624078(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T15H9.1
Entrez Gene 3565672 Entrez gene
SwissProt Q10005 EMBL-EBI
WormBase CE30689 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE57914 Hypothetical protein CBG00965 [Caenorhabditis briggsae] 1.0E-157
blast CAD31695 Hypothetical protein T15H9.7 [Caenorhabditis elegans] ref|NP_741036.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-20) [Caenorhabditis elegans] sp|Q8MPX3|DNJ20_CAEEL DnaJ homolog dnj-20 precursor (DnaJ domain protein 20) 1.0E-106
blast XP_422682 PREDICTED: similar to DnaJ homolog subfamily B member 11 precursor (ER-associated dnaJ protein 3) (ErJ3) (ER-associated Hsp40 co-chaperone) (hDj9) (PWP1-interacting protein 4) (UNQ537/PRO1080) [Gallus gallus] 1.0E-103
blast CAA87414 Hypothetical protein T15H9.1 [Caenorhabditis elegans] ref|NP_496072.2| T15H9.1 [Caenorhabditis elegans] sp|Q10005|YRY1_CAEEL Hypothetical protein T15H9.1 in chromosome II 4.0E-41
blast CAE57914 Hypothetical protein CBG00965 [Caenorhabditis briggsae] 1.0E-36
blast AAF51493 CG4164-PA [Drosophila melanogaster] gb|AAK93202.1| LD30318p [Drosophila melanogaster] ref|NP_608525.1| CG4164-PA [Drosophila melanogaster] 2.0E-22
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 5.00000009770741E-26
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 3.4E-6
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 1.6E-22
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 2.6E-36
Sequence
Target Sequence 
>C. ELEGANS:188257_AT
ggaactcgtcaatgtaactgccgtcacgagatgcgtaccgaacaaatgggacagggaagg
ttccaaatgttccaagtgaaagtttgtgacgagtgcccaaatgtgaagctggttcaggag
aataaagttttggaggttgaagtggaggttggagccgataatggtcatcaacaaattttc
cacggagagggagaaccacacattgaaggagatccaggagatttgaaattcaagattcga
attcagaagcatccaaggttcgaacgcaagggtgatgatctgtacacaaatgtgacaatt
tcacttcaagacgcgctcaacggcttcgaaatggaaattcaacatctcgacgggcacatt
gtcaaggttcaaagagataaggtaacctggccaggggcccgtcttcgtaagaaggacgaa
ggaatgccatcccttgaagataacaacaagaagggtatgcttgtcgtcacttttgatgtt
gaattccccaagacggagctgtcggacgaacagaaagcgcagatcatcgagattcttcaa
c

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACTCGTCAATGTAACTGCCGTC 388 529 502 Antisense
TGCCGTCACGAGATGCGTACCGAAC 518 585 520 Antisense
GGTCATCAACAAATTTTCCACGGAG 137 503 664 Antisense
GAAGCATCCAAGGTTCGAACGCAAG 653 341 747 Antisense
GACAATTTCACTTCAAGACGCGCTC 75 365 795 Antisense
CGCGCTCAACGGCTTCGAAATGGAA 533 255 813 Antisense
AAATTCAACATCTCGACGGGCACAT 516 115 836 Antisense
GGACGAAGGAATGCCATCCCTTGAA 296 521 915 Antisense
AGAAGGGTATGCTTGTCGTCACTTT 657 71 950 Antisense
AATTCCCCAAGACGGAGCTGTCGGA 524 175 983 Antisense
GCGCAGATCATCGAGATTCTTCAAC 52 289 1018 Antisense
Sequence Source Affymetrix Proprietary Database