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[188252_at]

Description F54D10.1 /REP_DB=WormBase Gene ID /WP=CE19451 /GEN=skr-15 /TR=Q9TZ83 /GB=AAC68782.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 77      4grp_total > 11      6grp_cgc > 49      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 40.2
(-3)
A 		43.7
(-2.6)
A 		48.7
(-2.1)
A 		97.2
(3)
A 		57
B 64.9
(-1.3)
A 		48
(-2.9)
A 		46.4
(-3)
A 		112.1
(3)
A 		66
C 45.2
(-1.3)
A 		62.8
(-0.4)
A 		13.2
(-3)
A 		125.6
(3)
M 		112
avr 50.1
(-1.9) 		51.5
(-1.8) 		36.1
(-3) 		111.6
(3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 375
1 2 3 4 5 6 range
A 8.4
(-3)
A 		60.8
(-2.1)
A 		50.3
(-2.2)
A 		39.6
(-2.4)
A 		108.4
(-1.2)
P 		339.7
(3)
P 		331
B 55
(-2.2)
A 		62.3
(-2)
A 		6.3
(-3)
A 		12.7
(-2.9)
A 		112.6
(-1.2)
P 		358.2
(3)
P 		352
C 39.7
(-2.5)
A 		21.8
(-2.8)
A 		75.5
(-2)
A 		11.6
(-3)
A 		128.8
(-1.1)
P 		381
(3)
P 		369
avr 34.4
(-2.8) 		48.3
(-2.5) 		44
(-2.6) 		21.3
(-3) 		116.6
(-1.3) 		359.6
(3) 		338
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 14.6
(3)
A 		9.5
(-3)
A 		12.7
(0.8)
A 		14.2
(2.5)
A 		5
B 20.7
(1.5)
A 		11.9
(-2.5)
A 		10.7
(-3)
A 		23.9
(3)
A 		13
C 15
(0.8)
A 		18
(1.8)
A 		4.6
(-3)
A 		21.2
(3)
M 		17
avr 16.8
(1.3) 		13.1
(-0.8) 		9.3
(-3) 		19.8
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 1.5
(-3)
A 		21.6
(-1.5)
A 		11
(-2.3)
A 		10
(-2.4)
A 		32.7
(-0.7)
P 		82.7
(3)
P 		81
B 19.2
(-2.2)
A 		20.7
(-2.1)
A 		1.5
(-3)
A 		3.4
(-2.9)
A 		45.7
(-1)
P 		133.6
(3)
P 		132
C 14.7
(-2.4)
A 		6.7
(-2.8)
A 		19.2
(-2.2)
A 		2.3
(-3)
A 		41.7
(-1.1)
P 		127.5
(3)
P 		125
avr 11.8
(-2.6) 		16.3
(-2.4) 		10.6
(-2.7) 		5.2
(-3) 		40
(-1.1) 		114.6
(3) 		109
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188252_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5562
Sequence Type Exemplar sequence
Representative Public ID F54D10.1 NCBI
Target Description F54D10.1 /REP_DB=WormBase Gene ID /WP=CE19451 /GEN=skr-15 /TR=Q9TZ83 /GB=AAC68782.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062261(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062261 NCBI Caenorhabditis elegans SKp1 Related (ubiquitin ligase complex component) family member (skr-15) (skr-15) mRNA, complete cds. 11/11 None
SNAP00000030975 ENSEMBL cdna:SNAP chromosome:CEL140:II:3824148:3824702:1 11/11 None
GENEFINDER00000030984 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3824148:3824702:1 11/11 None
F54D10.1 ENSEMBL cdna:known chromosome:CEL140:II:3824148:3824702:1 gene:F54D10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3824146-3824701(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol skr-15
Ensembl F54D10.1
Entrez Gene 173729 Entrez gene
SwissProt Q9TZ83 EMBL-EBI
WormBase CE19451 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68782 Skp1 related (ubiquitin ligase complex component) protein 15 [Caenorhabditis elegans] ref|NP_494662.1| SKp1 Related (ubiquitin ligase complex component) family member (skr-15) [Caenorhabditis elegans] gb|AAL34104.1| SKR-15 [Caenorhabditis elegans] 3.0E-84
blast AAF60641 Skp1 related (ubiquitin ligase complex component) protein 7 [Caenorhabditis elegans] ref|NP_504221.1| SKp1 Related (ubiquitin ligase complex component) family member (skr-7) [Caenorhabditis elegans] gb|AAL34097.1| SKR-7 [Caenorhabditis elegans] 5.0E-36
blast AAF60635 Skp1 related (ubiquitin ligase complex component) protein 14 [Caenorhabditis elegans] ref|NP_504220.3| SKp1 Related (ubiquitin ligase complex component) family member (skr-14) [Caenorhabditis elegans] 4.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR001232 EMBL-EBI SKP1 component 3.0E-25
Pfam IPR001232 EMBL-EBI SKP1 component 6.5E-20
Sequence
Target Sequence 
>C. ELEGANS:188252_AT
cccagtcgtctactacagcattgagtctaacgacagagtggtcttgaaaatcagcgagca
agccatcaaacaatctgcaactctctcgaactccatcaccaacctcggatacagcgctga
gaacgccgagtcaatggtcccgattccaatcgagaaggtcaatgggaaaactttgaagct
ggttgttgaatggtgcgagcatcacaaggccgacccggttccagaagcctatccatctgg
taacacagtccttccagtgtgggatcgcaagttcgtggacattgagcacgacgccctaac
cgatttggtgaacgcctcgaacttcctcgaagtcatgacacttttgacttattgctgcaa
gttcatcgcgggtttggcaaaaggaatgtcgccggaagagatgagagtgtttttctgcat
tccgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCAGTCGTCTACTACAGCATTGAG 321 259 60 Antisense
TCAAACAATCTGCAACTCTCTCGAA 664 629 125 Antisense
ACCAACCTCGGATACAGCGCTGAGA 256 85 157 Antisense
CGAGTCAATGGTCCCGATTCCAATC 65 249 186 Antisense
GAAGCCTATCCATCTGGTAACACAG 188 345 283 Antisense
ACAGTCCTTCCAGTGTGGGATCGCA 430 105 304 Antisense
GTGGGATCGCAAGTTCGTGGACATT 244 489 318 Antisense
GACGCCCTAACCGATTTGGTGAACG 431 379 349 Antisense
ATGACACTTTTGACTTATTGCTGCA 308 45 394 Antisense
ATTGCTGCAAGTTCATCGCGGGTTT 623 5 410 Antisense
TGAGAGTGTTTTTCTGCATTCCGAC 271 569 461 Antisense
Sequence Source Affymetrix Proprietary Database