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[188251_at]

Description T26C12.4 /REP_DB=WormBase Gene ID /WP=CE26010 /GEN=gcy-23 /TR=O61859 /GB=AAC17555.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 06      4grp_total > 14      6grp_cgc > 22      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 165
1 2 3 4 range
A 169.9
(1.3)
P 		100.3
(-3)
A 		111
(-2.3)
A 		198.4
(3)
A 		98
B 194.3
(2)
P 		91.2
(-1.7)
A 		56.8
(-3)
A 		221.9
(3)
P 		165
C 144.9
(0.3)
P 		77.4
(-2.9)
A 		75.5
(-3)
A 		203.2
(3)
P 		128
avr 169.7
(1.2) 		89.6
(-2.6) 		81.1
(-3) 		207.8
(3) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 120.5
(3)
P 		117.5
(2.7)
P 		88
(-0.1)
A 		75.1
(-1.4)
M 		66.6
(-2.2)
A 		58.3
(-3)
A 		62
B 164.5
(3)
P 		108.6
(0.1)
A 		75.1
(-1.6)
A 		47.3
(-3)
A 		90.2
(-0.8)
A 		77
(-1.5)
A 		117
C 199.9
(3)
P 		129.5
(-0.1)
A 		124
(-0.4)
M 		108.3
(-1)
A 		76.1
(-2.5)
P 		64.1
(-3)
A 		136
avr 161.6
(3) 		118.5
(0.3) 		95.7
(-1.2) 		76.9
(-2.3) 		77.6
(-2.3) 		66.5
(-3) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 61.9
(3)
P 		21.8
(-3)
A 		29
(-1.9)
A 		29
(-1.9)
A 		40
B 62
(3)
P 		22.5
(-1.9)
A 		13.2
(-3)
A 		47.3
(1.2)
P 		49
C 48
(3)
P 		22.3
(-3)
A 		26.3
(-2.1)
A 		34.3
(-0.2)
P 		26
avr 57.3
(3) 		22.2
(-3) 		22.8
(-2.9) 		36.9
(-0.5) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 22.2
(-1.3)
P 		41.8
(3)
P 		19.2
(-1.9)
A 		19
(-2)
M 		20.1
(-1.7)
A 		14.2
(-3)
A 		28
B 57.2
(3)
P 		36.1
(0.2)
A 		18.2
(-2.2)
A 		12.5
(-3)
A 		36.7
(0.2)
A 		28.7
(-0.8)
A 		45
C 74.1
(3)
P 		39.5
(-0.9)
A 		31.6
(-1.8)
M 		21.8
(-3)
A 		24.6
(-2.6)
P 		21.5
(-3)
A 		53
avr 51.2
(3) 		39.1
(0.8) 		23
(-2.1) 		17.8
(-3) 		27.1
(-1.3) 		21.5
(-2.3) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188251_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11208
Sequence Type Exemplar sequence
Representative Public ID T26C12.4 NCBI
Target Description T26C12.4 /REP_DB=WormBase Gene ID /WP=CE26010 /GEN=gcy-23 /TR=O61859 /GB=AAC17555.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067908(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067908 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-23) (gcy-23) mRNA, complete cds. 11/11 None
T26C12.4 ENSEMBL cdna:known chromosome:CEL140:IV:3282881:3288946:-1 gene:T26C12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3283010-3291210(-) 97.49 100.0
Public Domain and Genome References
Gene Title
Gene Symbol gcy-23
Ensembl T26C12.4
Entrez Gene 191652 Entrez gene
SwissProt O61859 EMBL-EBI
WormBase CE36614 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_500309 Guanylyl CYclase family member (gcy-23) [Caenorhabditis elegans] gb|AAC17555.3| Guanylyl cyclase protein 23 [Caenorhabditis elegans] 0.0
blast CAB05325 Hypothetical protein ZK896.8 [Caenorhabditis elegans] ref|NP_502449.1| Guanylyl CYclase family member (gcy-18) [Caenorhabditis elegans] 0.0
blast CAE56618 Hypothetical protein CBG24374 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.3.1.Di-heme cytochrome c peroxidase All alpha proteins; Cytochrome c; Cytochrome c; Di-heme cytochrome c peroxidase 3.5
Pfam IPR000719 EMBL-EBI Protein kinase 5.2E-9
Pfam IPR001054 EMBL-EBI Guanylate cyclase 3.7E-77
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 4.7E-66
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500309.2 1 428-450
T26C12.4 1 428-450
Sequence
Target Sequence 
>C. ELEGANS:188251_AT
gatccgtgccggcaaagacattcgatatggctacggtgatgttcagtgacatcgtgggct
tcacaacaatctgctcttcgtcgactccgctggaggttgtgagcatgttgaacagtatct
acagcaaatttgacgacgctattaataagcacggatcttataaggtcgaaacaatcgggg
acgcctacatgatcgtctctggaatccccgaagaaaacggcaacgagcacatcagaaaca
tctgcaacacggctctcgagctaatgcttctcctcaaaacctacgagatccctcatcgcc
gaaacgtgaagctccgcatccgccttggcatccacactggaaccgtcgcggcgggagtcg
tcggtcttaccgcacctcgttattgcctattcggtgacactgtaaatgttgcctcgagaa
tggagtctactagtgagccggagaagatccagatgagccaggaagctcgcgacttttgtg
tcaggtactactcggaattccaaatcaccctccgcggaaccgtcgaggcgaaaggcaaag
gaccagtgacgtcgtattggttgctcggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCCGTGCCGGCAAAGACATTCGA 136 417 2810 Antisense
GACATCGTGGGCTTCACAACAATCT 656 369 2857 Antisense
TTCGTCGACTCCGCTGGAGGTTGTG 624 611 2886 Antisense
GAAACAATCGGGGACGCCTACATGA 653 355 2977 Antisense
GCCTACATGATCGTCTCTGGAATCC 192 283 2992 Antisense
CACGGCTCTCGAGCTAATGCTTCTC 339 199 3057 Antisense
ATCCCTCATCGCCGAAACGTGAAGC 335 37 3097 Antisense
GTTATTGCCTATTCGGTGACACTGT 110 447 3188 Antisense
GAAGCTCGCGACTTTTGTGTCAGGT 448 343 3271 Antisense
TACTCGGAATTCCAAATCACCCTCC 211 645 3298 Antisense
GTGACGTCGTATTGGTTGCTCGGAA 639 481 3355 Antisense
Sequence Source Affymetrix Proprietary Database