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[188249_at] ATPase

Description R05C11.3 /REP_DB=WormBase Gene ID /WP=CE21090 /TR=Q9TYP9 /GB=AAD12806.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ATPase
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 29      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 60      6grp_total > 28      4grp_total > 53      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 291
1 2 3 4 range
A 733.1
(3)
P 		587.3
(-0.6)
P 		604.1
(-0.2)
P 		488.1
(-3)
P 		245
B 688
(3)
P 		599.2
(0.4)
P 		483.6
(-3)
P 		570.7
(-0.4)
P 		204
C 679
(1.8)
P 		738.3
(3)
P 		641.1
(1)
P 		447.8
(-3)
P 		291
avr 700
(3) 		641.6
(1.2) 		576.3
(-0.8) 		502.2
(-3) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 397
1 2 3 4 5 6 range
A 534.4
(2.9)
P 		526
(2.7)
P 		270.5
(-3)
P 		368.1
(-0.8)
P 		435.1
(0.7)
P 		537.6
(3)
P 		267
B 555.6
(3)
P 		439.6
(0.5)
P 		275.9
(-3)
P 		301
(-2.5)
P 		322.4
(-2)
P 		394.9
(-0.4)
P 		280
C 667.3
(3)
P 		453.2
(-0.3)
P 		433.1
(-0.6)
P 		280.4
(-3)
P 		570.6
(1.5)
P 		301.9
(-2.7)
P 		387
avr 585.8
(3) 		472.9
(0.5) 		326.5
(-2.8) 		316.5
(-3) 		442.7
(-0.2) 		411.5
(-0.9) 		269
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 266.9
(3)
P 		127.5
(-1.3)
P 		157.9
(-0.3)
P 		71.4
(-3)
P 		196
B 219.4
(3)
P 		148
(-1)
P 		112.1
(-3)
P 		121.6
(-2.5)
P 		107
C 224.8
(3)
P 		212.3
(2.5)
P 		223.4
(2.9)
P 		75.5
(-3)
P 		149
avr 237
(3) 		162.6
(-0) 		164.5
(0.1) 		89.5
(-3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 191
1 2 3 4 5 6 range
A 98.5
(-1.1)
P 		187.1
(3)
P 		58.9
(-3)
P 		93.4
(-1.4)
P 		131.3
(0.4)
P 		130.9
(0.4)
P 		128
B 193.3
(3)
P 		146.3
(0.8)
P 		67
(-3)
P 		79.7
(-2.4)
P 		131
(0)
P 		147.3
(0.8)
P 		126
C 247.4
(3)
P 		138.3
(-0.4)
P 		110.2
(-1.3)
P 		56.4
(-3)
P 		184.6
(1)
P 		101.1
(-1.6)
P 		191
avr 179.7
(3) 		157.2
(1.7) 		78.7
(-2.9) 		76.5
(-3) 		149
(1.2) 		126.4
(-0.1) 		103
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188249_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10906
Sequence Type Exemplar sequence
Representative Public ID R05C11.3 NCBI
Target Description R05C11.3 /REP_DB=WormBase Gene ID /WP=CE21090 /TR=Q9TYP9 /GB=AAD12806.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ATPase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067760(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067760 NCBI Caenorhabditis elegans R05C11.3 (ATPase) mRNA, complete cds. 11/11 None
SNAP00000026668 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2085251:2090149:1 10/11 None
GENEFINDER00000026669 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2084050:2090149:1 10/11 None
R05C11.3 ENSEMBL cdna:known chromosome:CEL140:IV:2084033:2091921:1 gene:R05C11.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2084055-2091409(+) 100.0 100.0
Public Domain and Genome References
Gene Title ATPase
Ensembl R05C11.3
Entrez Gene 177004 Entrez gene
SwissProt Q9TYP9 EMBL-EBI
WormBase CE28747 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18296_AT calcium-transporting ATPase 4, plasma membrane-type / Ca2+-ATPase, isoform 4 (ACA4) at
ATH1-121501:245117_AT calcium-transporting ATPase 4, plasma membrane-type / Ca2+-ATPase, isoform 4 (ACA4) at
CANINE:1594040_AT similar to plasma membrane calcium ATPase 2 isoform b cfa
CANINE:1592543_AT similar to plasma membrane calcium ATPase 2 isoform b cfa
CANINE_2:CFA.9608.1.A1_AT similar to plasma membrane calcium ATPase 2 isoform b cfa
CANINE_2:CFAAFFX.8587.1.S1_AT similar to plasma membrane calcium ATPase 2 isoform b cfa
CANINE_2:CFAAFFX.8587.1.S1_S_AT similar to plasma membrane calcium ATPase 2 isoform b cfa
CHICKEN:GGAAFFX.3001.1.S1_AT similar to plasma membrane calcium ATPase 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase isoform 2 Na+K+ transporting beta polypeptide 2 gga
CHICKEN:GGAAFFX.3004.1.S1_AT similar to plasma membrane calcium ATPase 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase isoform 2 Na+K+ transporting beta polypeptide 2 gga
HG-U95AV2:37053_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U95AV2:41090_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133_PLUS_2:211586_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133A:211586_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133A_2:211586_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HU35KSUBA:L20977_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HUGENEFL:X63575_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-FOCUS:204685_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133A:204685_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133A_2:204685_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133_PLUS_2:204685_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
U133_X3P:G190098_3P_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
U133_X3P:HS.89512.3.S1_3P_AT ATPase, Ca++ transporting, plasma membrane 2 hs
U133_X3P:HS.89512.0.S2_3P_A_AT ATPase, Ca++ transporting, plasma membrane 2 hs
U133_X3P:HS.305923.3.S1_3P_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133A_2:216120_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133_PLUS_2:216120_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133A:216120_S_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133_PLUS_2:234714_X_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133B:234714_X_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U95C:59397_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133_PLUS_2:234741_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U133B:234741_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HG-U95B:59803_AT ATPase, Ca++ transporting, plasma membrane 2 hs
HU35KSUBC:RC_H62161_AT ATPase, Ca++ transporting, plasma membrane 2 hs
MOE430A:1420402_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MOUSE430_2:1420402_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MOUSE430A_2:1420402_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MOUSE430_2:1420403_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MOE430A:1420403_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MOUSE430A_2:1420403_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MU11KSUBB:MSA.28504.0_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MG-U74AV2:95324_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MG-U74CV2:138539_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MOE430A:1433888_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MOUSE430A_2:1433888_AT ATPase, Ca++ transporting, plasma membrane 2 mm
MOUSE430_2:1433888_AT ATPase, Ca++ transporting, plasma membrane 2 mm
RN-U34:L05557CDS_G_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RG-U34A:L05557CDS_G_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RN-U34:J03754COMPLETE_SEQ_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RG-U34A:J03754COMPLETE_SEQ_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RG-U34A:L05557CDS_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RAE230A:1368698_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RAT230_2:1368698_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RAE230A:1387285_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RAT230_2:1387285_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RG-U34A:RC_AA943784_S_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RN-U34:RC_AA943784_S_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RN-U34:RC_AA955388_S_AT ATPase, Ca++ transporting, plasma membrane 2 rn
RG-U34A:RC_AA955388_S_AT ATPase, Ca++ transporting, plasma membrane 2 rn
YEAST_2:1770017_AT Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6812 cation transport inferred from electronic annotation QuickGO AmiGO
6816 calcium ion transport inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
5388 calcium-transporting ATPase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
15085 calcium ion transporter activity inferred from electronic annotation QuickGO AmiGO
15662 ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism inferred from electronic annotation QuickGO AmiGO
16820 hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_500161 R05C11.3 [Caenorhabditis elegans] gb|AAD12806.2| Hypothetical protein R05C11.3 [Caenorhabditis elegans] 0.0
blast CAA09308 calcium ATPase [Caenorhabditis elegans] 0.0
blast CAE74692 Hypothetical protein CBG22506 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A1A4_HUMAN (Q13733) Sodium/potassium-transporting ATPase alpha-4 chain (EC 3.6.3.9) (Sodium pump 4) (Na+/K+ ATPase 4) 0.0
ec A1A2_HUMAN (P50993) Sodium/potassium-transporting ATPase alpha-2 chain precursor (EC 3.6.3.9) (Sodium pump 2) (Na+/K+ ATPase 2) 1.0E-180
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 3.8E-21
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 2.9E-25
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 4.3E-21
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 4.9E-16
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 1.1E-19
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 3.8E-21
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 4.3E-21
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 4.9E-16
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500161.1 10 89-107,122-144,326-348,368-390,816-838,848-867,888-910,930-952,964-986,1001-1023
R05C11.3 10 89-107,122-144,326-348,368-390,816-838,848-867,888-910,930-952,964-986,1001-1023
SNAP00000026668 8 7-26,83-105,126-148,578-600,650-672,692-714,726-748,763-785
GENEFINDER00000026669 10 89-107,122-144,326-348,368-390,816-838,848-867,888-910,930-952,964-986,1001-1023
Sequence
Target Sequence 
>C. ELEGANS:188249_AT
ttcttttcgcgatcatgttctggggagacaaactgattccaaatactccatccggaagaa
acgccgagttgggatcaccaccaagtgctcacttcacaattattttcaacgcttttgtct
tgatgacgttggtaaatgagatcaatgccaggaagattcatggagagagaaatgttttca
agggaattttcaccaacccaatcttctgtgtaatctggatcactactctcatctcgcaca
tcctcatcgtccagttcggaggtcaatggttctccaccgcaccgctagacttgacacaat
ggatcatttgtatttgttgcggagtcggcgagctcttctggggacagattatcaactgca
ttcccgcatcgattctcccgaagtcattccgattcggaaagggagacgttcaaccgacgt
cgatcatgctctccggagagtatgatatgccaagcaccgcgtcgacgctgccaatgaagg
atggcgcggccgctgaccatcagagagccggacaatcgctctggttgctcggtttgacca
ggttacagacgcaggggctggtggccta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTTTTCGCGATCATGTTCTGGGG 512 689 2720 Antisense
CCAACCCAATCTTCTGTGTAATCTG 270 277 2912 Antisense
TAATCTGGATCACTACTCTCATCTC 559 635 2930 Antisense
CATCCTCATCGTCCAGTTCGGAGGT 206 211 2958 Antisense
GTATTTGTTGCGGAGTCGGCGAGCT 313 453 3029 Antisense
AGATTATCAACTGCATTCCCGCATC 538 65 3065 Antisense
ATTCTCCCGAAGTCATTCCGATTCG 410 9 3091 Antisense
CGGCCGCTGACCATCAGAGAGCCGG 342 241 3206 Antisense
AGAGCCGGACAATCGCTCTGGTTGC 583 67 3223 Antisense
GGTTGCTCGGTTTGACCAGGTTACA 649 507 3242 Antisense
TACAGACGCAGGGGCTGGTGGCCTA 269 641 3263 Antisense
Sequence Source Affymetrix Proprietary Database