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[188248_at] CDC2

Description B0495.2 /REP_DB=WormBase Gene ID /WP=CE01761 /TR=SW:Q09437 /GB=AAA62523.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=CDC2
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 66      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 75      6grp_total > 66      4grp_total > 47      6grp_highest_sub > Da      6grp_high1_sub > Da      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 438
1 2 3 4 range
A 1036.8
(3)
P 		657.2
(-2.4)
P 		612.6
(-3)
P 		625.2
(-2.8)
P 		424
B 1025.5
(3)
P 		676.7
(-2.8)
P 		677.7
(-2.7)
P 		661.7
(-3)
P 		364
C 1050.7
(3)
P 		651.6
(-3)
P 		773.1
(-1.2)
P 		731.7
(-1.8)
P 		399
avr 1037.7
(3) 		661.8
(-3) 		687.8
(-2.6) 		672.9
(-2.8) 		376

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 544
1 2 3 4 5 6 range
A 859.7
(-1.2)
P 		905.5
(-0.6)
P 		792.3
(-2.1)
P 		1034.7
(1.1)
P 		727.5
(-3)
P 		1178.3
(3)
P 		451
B 885.2
(0.8)
P 		634.3
(-3)
P 		924.7
(1.4)
P 		710.4
(-1.8)
P 		681.2
(-2.3)
P 		1029.3
(3)
P 		395
C 771.5
(-2.6)
P 		748.3
(-3)
P 		961.5
(0.3)
P 		953.4
(0.2)
P 		842
(-1.5)
P 		1134.6
(3)
P 		386
avr 838.8
(-1.5) 		762.7
(-2.8) 		892.8
(-0.6) 		899.5
(-0.5) 		750.2
(-3) 		1114.1
(3) 		364
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 286
1 2 3 4 range
A 377.5
(3)
P 		142.7
(-1.9)
P 		160.1
(-1.6)
P 		91.5
(-3)
P 		286
B 327
(3)
P 		167.1
(-2.2)
P 		157
(-2.5)
P 		141
(-3)
P 		186
C 347.8
(3)
P 		187.3
(-1.3)
P 		269.5
(0.9)
P 		123.4
(-3)
P 		224
avr 350.8
(3) 		165.7
(-1.8) 		195.5
(-1) 		118.6
(-3) 		232

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 225
1 2 3 4 5 6 range
A 158.5
(-3)
P 		322
(3)
P 		172.6
(-2.5)
P 		262.4
(0.8)
P 		219.5
(-0.8)
P 		286.9
(1.7)
P 		164
B 308
(0.7)
P 		211.1
(-2.3)
P 		224.6
(-1.9)
P 		188.2
(-3)
P 		276.8
(-0.3)
P 		383.8
(3)
P 		196
C 286
(0)
P 		228.3
(-1.8)
P 		244.7
(-1.3)
P 		191.7
(-3)
P 		272.5
(-0.4)
P 		379.8
(3)
P 		188
avr 250.8
(-1.4) 		253.8
(-1.2) 		214
(-3) 		214.1
(-3) 		256.3
(-1.1) 		350.2
(3) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188248_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4622
Sequence Type Exemplar sequence
Representative Public ID B0495.2 NCBI
Target Description B0495.2 /REP_DB=WormBase Gene ID /WP=CE01761 /TR=SW:Q09437 /GB=AAA62523.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=CDC2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063216(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063216 NCBI Caenorhabditis elegans B0495.2 (CDC2) mRNA, complete cds. 11/11 None
SNAP00000031531 ENSEMBL cdna:SNAP chromosome:CEL140:II:7699228:7701845:1 11/11 None
GENEFINDER00000031541 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7699228:7701845:1 11/11 None
B0495.2 ENSEMBL cdna:known chromosome:CEL140:II:7699215:7701912:1 gene:B0495.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7699224-7701842(+) 100.0 100.0
Public Domain and Genome References
Gene Title CDC2
Ensembl B0495.2
Entrez Gene 174244 Entrez gene
SwissProt Q09437 EMBL-EBI
WormBase CE01761 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1606155_AT similar to cell division cycle 2-like 1 (PITSLRE proteins) isoform 1 cfa
CANINE_2:CFA.21616.1.S1_AT similar to cell division cycle 2-like 1 (PITSLRE proteins) isoform 1 cfa
CANINE_2:CFA.21616.1.S1_S_AT similar to cell division cycle 2-like 1 (PITSLRE proteins) isoform 1 cfa
DROSOPHILA_2:1629503_S_AT dm
DROSOPHILA_2:1633625_S_AT dm
DROSGENOME1:152617_AT dm
CHICKEN:GGA.16336.1.S1_AT similar to protein kinase gga
CHICKEN:GGA.18485.1.S1_S_AT similar to protein kinase gga
CHICKEN:GGAAFFX.24037.1.S1_S_AT similar to protein kinase gga
MOUSE430_2:1418841_S_AT cell division cycle 2-like 1 mm
MOE430A:1418841_S_AT cell division cycle 2-like 1 mm
MOUSE430A_2:1418841_S_AT cell division cycle 2-like 1 mm
MU11KSUBA:L37092_S_AT cell division cycle 2-like 1 mm
MG-U74AV2:99961_S_AT cell division cycle 2-like 1 mm
MG-U74AV2:95805_AT cell division cycle 2-like 1 mm
MG-U74AV2:161457_AT cell division cycle 2-like 1 mm
RG-U34C:RC_AA817907_AT similar to cell division cycle 2-like 1 rn
RAE230A:1398957_AT similar to cell division cycle 2-like 1 rn
RAT230_2:1398957_AT similar to cell division cycle 2-like 1 rn
RAE230B:1390757_AT similar to cell division cycle 2-like 1 rn
RAT230_2:1390757_AT similar to cell division cycle 2-like 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA62523 Hypothetical protein B0495.2 [Caenorhabditis elegans] ref|NP_495617.1| B0495.2 [Caenorhabditis elegans] sp|Q09437|YP62_CAEEL Putative serine/threonine-protein kinase B0495.2 0.0
blast CAE67480 Hypothetical protein CBG12984 [Caenorhabditis briggsae] 0.0
blast CAA90342 Hypothetical protein ZC504.3 [Caenorhabditis elegans] ref|NP_509746.1| ZC504.3 [Caenorhabditis elegans] 1.0E-155
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-70
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 9.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-87
Sequence
Target Sequence 
>C. ELEGANS:188248_AT
gttgtaactctctggtatcgatcaccggaattgcttcttggtacacgtctctattcaact
cccgttgatatgtggtctgttggatgtattatggcagagtttattctcttaaaaccattg
ttcccgggacgcggtgaacttgaacaaattaagaaaattttcatggaaatgggaactccc
acggaatcaatatggcctggagtcactgaactcgacggttggaaggcattgacatttgag
aagtatccatataatcaattgagaaagaggttcctagctggaagacttctcaatgacact
ggttttaagttacttaatggcctactcactctggacccaaagaatcgattctccgctaca
caagctcttgatcacgaatggttcactgaagaaccttatccagtgccaccggaggagttt
ccaacgtttcccgctaaaagcgaacagaataaagctccaccgccggcgaagcagaaacaa
caagaaaatcgcatttctcatgtggatccagaaactgccaagctcctaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGTAACTCTCTGGTATCGATCAC 467 437 1570 Antisense
GATCACCGGAATTGCTTCTTGGTAC 702 419 1589 Antisense
TTGGTACACGTCTCTATTCAACTCC 177 709 1607 Antisense
TGTTCCCGGGACGCGGTGAACTTGA 528 147 1688 Antisense
GGCCTGGAGTCACTGAACTCGACGG 221 535 1763 Antisense
TACTTAATGGCCTACTCACTCTGGA 593 643 1880 Antisense
TCTCCGCTACACAAGCTCTTGATCA 365 611 1919 Antisense
TATCCAGTGCCACCGGAGGAGTTTC 128 651 1966 Antisense
CGAACAGAATAAAGCTCCACCGCCG 410 253 2010 Antisense
ATCGCATTTCTCATGTGGATCCAGA 540 37 2057 Antisense
GATCCAGAAACTGCCAAGCTCCTAA 622 415 2074 Antisense
Sequence Source Affymetrix Proprietary Database