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[188241_at]

Description F55A12.3 /REP_DB=WormBase Gene ID /WP=CE11121 /GEN=ppk-1 /TR=O01759 /GB=AAB54130.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 04      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 51      6grp_total > 15      4grp_total > 55      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 382
1 2 3 4 range
A 734.3
(3)
P 		564.2
(-0.4)
P 		522.6
(-1.2)
P 		430.9
(-3)
P 		303
B 812.9
(3)
P 		469.3
(-3)
P 		528
(-2)
P 		469.4
(-3)
P 		344
C 770.8
(3)
P 		593.9
(-1.4)
P 		589.1
(-1.6)
P 		531.4
(-3)
P 		239
avr 772.7
(3) 		542.5
(-1.7) 		546.6
(-1.6) 		477.2
(-3) 		296

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 412
1 2 3 4 5 6 range
A 602
(0.4)
P 		735
(3)
P 		432.8
(-3)
P 		511
(-1.4)
P 		486.6
(-1.9)
P 		478.1
(-2.1)
P 		302
B 626.3
(3)
P 		562.7
(1.1)
P 		588.7
(1.9)
P 		471.6
(-1.6)
P 		440.1
(-2.5)
P 		424.5
(-3)
P 		202
C 796.2
(3)
P 		594.1
(0.1)
P 		570.4
(-0.3)
P 		527.1
(-0.9)
P 		497.3
(-1.4)
P 		384.3
(-3)
P 		412
avr 674.8
(3) 		630.6
(1.9) 		530.6
(-0.5) 		503.2
(-1.2) 		474.7
(-1.9) 		429
(-3) 		246
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 267.4
(3)
P 		122.5
(-1.3)
P 		136.6
(-0.8)
P 		63.1
(-3)
P 		204
B 259.2
(3)
P 		115.9
(-2.4)
P 		122.3
(-2.2)
P 		100
(-3)
P 		159
C 255.2
(3)
P 		170.7
(-0.1)
P 		205.3
(1.2)
P 		89.6
(-3)
P 		166
avr 260.6
(3) 		136.4
(-1.2) 		154.7
(-0.6) 		84.2
(-3) 		176

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 201
1 2 3 4 5 6 range
A 111
(-2.4)
P 		261.4
(3)
P 		94.3
(-3)
P 		129.6
(-1.7)
P 		146.8
(-1.1)
P 		116.4
(-2.2)
P 		167
B 217.9
(3)
P 		187.3
(1)
P 		142.9
(-1.8)
P 		124.9
(-3)
P 		178.8
(0.5)
P 		158.3
(-0.8)
P 		93
C 295.1
(3)
P 		181.3
(-0.6)
P 		145.2
(-1.8)
P 		106
(-3)
P 		160.9
(-1.3)
P 		128.6
(-2.3)
P 		189
avr 208
(2.9) 		210
(3) 		127.5
(-2.5) 		120.2
(-3) 		162.2
(-0.2) 		134.4
(-2.1) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188241_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1822
Sequence Type Exemplar sequence
Representative Public ID F55A12.3 NCBI
Target Description F55A12.3 /REP_DB=WormBase Gene ID /WP=CE11121 /GEN=ppk-1 /TR=O01759 /GB=AAB54130.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059175(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059175 NCBI Caenorhabditis elegans PIP Kinase family member (ppk-1) (ppk-1) mRNA, complete cds. 10/11 None
F55A12.3 ENSEMBL cdna:known chromosome:CEL140:I:5358523:5365173:1 gene:F55A12.3 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000029056 7/11 Cross Hyb Matching Probes None
SNAP00000029045 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5358527-5364803(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ppk-1
Ensembl F55A12.3
Entrez Gene 172184 Entrez gene
SwissProt O01759 EMBL-EBI
WormBase CE30541 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.29304.1.S1_S_AT similar to phosphatidylinositol-4-phosphate 5-kinase, type I, gamma cfa
CANINE_2:CFAAFFX.29305.1.S1_AT similar to phosphatidylinositol-4-phosphate 5-kinase, type I, gamma cfa
DROSOPHILA_2:1639668_AT dm
DROSOPHILA_2:1630829_AT dm
DROSOPHILA_2:1622913_A_AT dm
DROSGENOME1:151562_AT dm
DROSGENOME1:147724_AT dm
DROSGENOME1:142409_AT dm
CHICKEN:GGAAFFX.515.1.S1_AT similar to phosphatidylinositol-4-phosphate 5-kinase, type I, gamma; phosphatidylinositol-4-phosphate 5-kinase I-gamma; diphosphoinositide kinase; type I PIP kinase; PtdIns(4)P-5-kinase gga
CHICKEN:GGAAFFX.515.2.S1_S_AT similar to phosphatidylinositol-4-phosphate 5-kinase, type I, gamma; phosphatidylinositol-4-phosphate 5-kinase I-gamma; diphosphoinositide kinase; type I PIP kinase; PtdIns(4)P-5-kinase gga
HG-U133_PLUS_2:212518_AT phosphatidylinositol-4-phosphate 5-kinase, type I, gamma hs
HG-U133A_2:212518_AT phosphatidylinositol-4-phosphate 5-kinase, type I, gamma hs
HG-U133A:212518_AT phosphatidylinositol-4-phosphate 5-kinase, type I, gamma hs
U133_X3P:HS.275182.0.S1_3P_AT phosphatidylinositol-4-phosphate 5-kinase, type I, gamma hs
HG-U95AV2:32133_AT phosphatidylinositol-4-phosphate 5-kinase, type I, gamma hs
MOE430A:1450228_A_AT phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma mm
MOUSE430A_2:1450228_A_AT phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma mm
MOUSE430_2:1450228_A_AT phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma mm
MG-U74AV2:94119_AT phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma mm
MOE430A:1424954_A_AT phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma mm
MOUSE430A_2:1424954_A_AT phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma mm
MOUSE430_2:1424954_A_AT phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma mm
MG-U74BV2:105266_AT Phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma, mRNA (cDNA clone MGC:29231 IMAGE:5041431) mm
MG-U74BV2:109785_AT Phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma, mRNA (cDNA clone MGC:29231 IMAGE:5041431) mm
MU19KSUBA:TC17541_AT Phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma, mRNA (cDNA clone MGC:29231 IMAGE:5041431) mm
RAE230A:1372861_AT Phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (predicted) rn
RAT230_2:1372861_AT Phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (predicted) rn
RG-U34C:AA942675_AT Phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (predicted) rn
RG-U34A:RC_AA799473_AT Phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16308 1-phosphatidylinositol-4-phosphate 5-kinase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB54130 Pip kinase protein 1 [Caenorhabditis elegans] ref|NP_491576.2| PIP Kinase family member (ppk-1) [Caenorhabditis elegans] 0.0
blast CAE66932 Hypothetical protein CBG12324 [Caenorhabditis briggsae] 0.0
blast AAF46920 CG3682-PA, isoform A [Drosophila melanogaster] ref|NP_611729.2| CG3682-PA, isoform A [Drosophila melanogaster] 1.0E-141
Protein Domains
Database ID Description E-Value
Pfam IPR002498 EMBL-EBI Phosphatidylinositol-4-phosphate 5-kinase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188241_AT
ggggacacaatttccgtgcacaacccaaacttctacgcatctcgatttttaacttttatg
accgaaaaagttttcaaaaagggaacagctctgaaacagtcgccatctcgtagacggatg
ttaaaaggaaatagtcatgaagatgattcaattgttattgtaagcagtggacatcgacaa
catcgagaatctgaacgagatggattgaatatgacggttacagtaaccgatggagcgagt
acatcaacaccacggggagataccacacaatttggatctcaaacagcacgagaatctact
agactatataatagagattctacaggaatcaatgaggatcgggatctcaaaaaccgaaca
aaaatttttcagatttttagagaatttttcttcaaaattttcaaaattgggataatttat
tctaatttcattttcttcgagcaactcgattatagttctattgcatccaccattgccgtt
cattcttcttcttccgacatagtcttcagtttcaaccaaaatctgccaatgttcctttca
aa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGGACACAATTTCCGTGCACAACC 315 499 1300 Antisense
CAAACTTCTACGCATCTCGATTTTT 505 191 1325 Antisense
GGAACAGCTCTGAAACAGTCGCCAT 415 529 1381 Antisense
ACAGTCGCCATCTCGTAGACGGATG 224 107 1395 Antisense
GTAAGCAGTGGACATCGACAACATC 158 463 1459 Antisense
TGGAGCGAGTACATCAACACCACGG 392 547 1530 Antisense
AACACCACGGGGAGATACCACACAA 98 97 1545 Antisense
AATTTCATTTTCTTCGAGCAACTCG 145 175 1723 Antisense
TATAGTTCTATTGCATCCACCATTG 350 661 1750 Antisense
CTTCTTCTTCCGACATAGTCTTCAG 535 221 1784 Antisense
AAAATCTGCCAATGTTCCTTTCAAA 260 133 1817 Antisense
Sequence Source Affymetrix Proprietary Database