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[188239_s_at]

Description F35G12.6 /REP_DB=WormBase Gene ID /WP=CE00975 /GEN=mab-21 /TR=Q20054 /GB=CAA86330.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 23      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 62      6grp_total > 40      4grp_total > 58      6grp_highest_sub > 02      6grp_cgc > 50      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 380
1 2 3 4 range
A 434
(-1.4)
P 		676.6
(2.7)
P 		695.3
(3)
P 		336.9
(-3)
P 		358
B 468.1
(-1.1)
P 		676.2
(3)
P 		631.5
(2.1)
P 		370.1
(-3)
P 		306
C 464.1
(-0.5)
P 		615.8
(2)
P 		678
(3)
P 		315.7
(-3)
P 		362
avr 455.4
(-0.9) 		656.2
(2.8) 		668.3
(3) 		340.9
(-3) 		327

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 343
1 2 3 4 5 6 range
A 601.4
(3)
P 		593.3
(2.8)
P 		556.7
(2.1)
P 		587.2
(2.7)
P 		396.4
(-0.9)
P 		289
(-3)
P 		312
B 504.1
(1.6)
P 		562.4
(3)
P 		373.3
(-1.4)
P 		541.1
(2.5)
P 		336
(-2.3)
P 		305.1
(-3)
P 		257
C 507.2
(1)
P 		611.2
(2.8)
P 		622.7
(3)
P 		598.2
(2.6)
P 		368.2
(-1.5)
P 		280
(-3)
P 		343
avr 537.6
(2) 		589
(3) 		517.6
(1.6) 		575.5
(2.7) 		366.9
(-1.5) 		291.4
(-3) 		298
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 187
1 2 3 4 range
A 158
(1.9)
P 		146.9
(1.4)
P 		181.8
(3)
P 		49.3
(-3)
P 		133
B 149.3
(1.8)
P 		167
(3)
P 		146.3
(1.6)
P 		78.9
(-3)
P 		88
C 153.6
(0.3)
P 		177
(1.1)
P 		236.3
(3)
P 		53.2
(-3)
P 		183
avr 153.6
(1.4) 		163.6
(1.8) 		188.1
(3) 		60.5
(-3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 141
1 2 3 4 5 6 range
A 110.9
(-1.3)
P 		211
(3)
P 		121.2
(-0.8)
P 		148.9
(0.3)
P 		119.6
(-0.9)
P 		70.4
(-3)
P 		141
B 175.4
(2.3)
P 		187.2
(3)
P 		90.7
(-3)
P 		143.3
(0.3)
P 		136.5
(-0.2)
P 		113.8
(-1.6)
P 		97
C 188
(3)
P 		186.5
(2.9)
P 		158.5
(1.1)
P 		120.3
(-1.3)
P 		119.2
(-1.4)
P 		93.7
(-3)
P 		94
avr 158.1
(0.8) 		194.9
(3) 		123.5
(-1.2) 		137.5
(-0.4) 		125.1
(-1.1) 		92.6
(-3) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188239_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6147
Sequence Type Exemplar sequence
Representative Public ID F35G12.6 NCBI
Target Description F35G12.6 /REP_DB=WormBase Gene ID /WP=CE00975 /GEN=mab-21 /TR=Q20054 /GB=CAA86330.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065539(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065539 NCBI Caenorhabditis elegans Male ABnormal family member (mab-21) (mab-21) mRNA, complete cds. 11/11 None
GENEFINDER00000042251 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4600785:4602933:1 11/11 None
F35G12.6 ENSEMBL cdna:known chromosome:CEL140:III:4600702:4603017:1 gene:F35G12.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000042248 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4600784-4602933(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mab-21
Ensembl F35G12.6
Entrez Gene 175607 Entrez gene
SwissProt Q20054 EMBL-EBI
WormBase CE32651 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.10370.1.S1_AT similar to mab-21-like 1 cfa
DROSOPHILA_2:1628477_S_AT dm
DROSGENOME1:144567_AT dm
CHICKEN:GGA.32.1.S1_AT mab-21-like 1 (C. elegans) gga
HG-U133_PLUS_2:206163_AT mab-21-like 1 (C. elegans) hs
HG-U133A:206163_AT mab-21-like 1 (C. elegans) hs
HG-FOCUS:206163_AT mab-21-like 1 (C. elegans) hs
HG-U133A_2:206163_AT mab-21-like 1 (C. elegans) hs
HUGENEFL:U38810_AT mab-21-like 1 (C. elegans) hs
U133_X3P:G5031896_3P_AT mab-21-like 1 (C. elegans) hs
HG-U95AV2:39297_AT mab-21-like 1 (C. elegans) hs
MOUSE430_2:1421369_A_AT mab-21-like 1 (C. elegans) mm
MOE430A:1421369_A_AT mab-21-like 1 (C. elegans) mm
MOUSE430A_2:1421369_A_AT mab-21-like 1 (C. elegans) mm
MG-U74CV2:165815_R_AT mab-21-like 1 (C. elegans) mm
MOE430A:1424679_AT mab-21-like 1 (C. elegans) mm
MOUSE430A_2:1424679_AT mab-21-like 1 (C. elegans) mm
MOUSE430_2:1424679_AT mab-21-like 1 (C. elegans) mm
MOE430B:1440860_AT mab-21-like 1 (C. elegans) mm
MOUSE430_2:1440860_AT mab-21-like 1 (C. elegans) mm
MOE430B:1457290_AT mab-21-like 1 (C. elegans) mm
MOUSE430_2:1457290_AT mab-21-like 1 (C. elegans) mm
MU19KSUBA:TC16042_AT Mab-21-like 1 (C. elegans), mRNA (cDNA clone MGC:25223 IMAGE:4526962) mm
Protein Similarities
Method ID Description E-Value
blast CAA86330 Hypothetical protein F35G12.6 [Caenorhabditis elegans] ref|NP_497940.2| Male ABnormal family member (mab-21) [Caenorhabditis elegans] gb|AAA97554.2| mab-21 protein [Caenorhabditis elegans] 0.0
blast AAG36973 MAB-21 protein [Caenorhabditis briggsae] 0.0
blast CAE71173 Hypothetical protein CBG18030 [Caenorhabditis briggsae] 1.0E-145
blast CAE71173 Hypothetical protein CBG18030 [Caenorhabditis briggsae] 1.0E-139
Protein Domains
Database ID Description E-Value
Pfam IPR004962 EMBL-EBI Mab-21 protein 1.0E-126
Pfam IPR004962 EMBL-EBI Mab-21 protein 1.0E-126
Pfam IPR004962 EMBL-EBI Mab-21 protein 6.3E-39
Sequence
Target Sequence 
>C. ELEGANS:188239_S_AT
ggtcgagttcattactgcttctggatatttatcagctcgcaagattcgccaccgattcca
aaatattgtggctcaagttttacaaactccacaattcaggacttccatggccgaacgccg
cgttggcgaatcagacaaaagccgagggattcgacttgacgagtcgtgaaactgcaatca
ctcaacaaaataatccgaataagcaagcgagcagtatggaagccgatgcctgggcaatga
agatgcatggtgcagagaacatgttactaactggaggtcggcggaagacattgagcattc
tgaaatgtcttcgagatgctcacatggactttccgggaacaccagtaacaaactacatac
tgaagacattggtattgtacgaatgtgagaagcactgtagtgaatacgaatgggaagatc
caaatatcggagatcgccttgtcggaattcttctacaactagtcagctgcctccaatgtc
gtcgctgtgctcactatttccttccatctttggatcttttacgttcaaaaccagtccatt
ccattgaacattccgcccaactcgcttggcatctcgttcgcaaactaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCGAGTTCATTACTGCTTCTGGA 615 501 353 Antisense
TTTATCAGCTCGCAAGATTCGCCAC 186 675 380 Antisense
ATTCGCCACCGATTCCAAAATATTG 474 9 396 Antisense
GTATGGAAGCCGATGCCTGGGCAAT 26 455 566 Antisense
AGATGCTCACATGGACTTTCCGGGA 291 65 666 Antisense
ATCGGAGATCGCCTTGTCGGAATTC 478 33 778 Antisense
TCTTCTACAACTAGTCAGCTGCCTC 140 617 801 Antisense
TCACTATTTCCTTCCATCTTTGGAT 601 625 843 Antisense
TACGTTCAAAACCAGTCCATTCCAT 265 649 872 Antisense
AGTCCATTCCATTGAACATTCCGCC 318 61 885 Antisense
TTGGCATCTCGTTCGCAAACTAATG 414 709 918 Antisense
Sequence Source Affymetrix Proprietary Database