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[188238_at] tubulin

Description F16D3.1 /REP_DB=WormBase Gene ID /WP=CE09434 /TR=Q19490 /GB=CAB01495.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=tubulin
Cluster 6grp_high1_10_10 > 80      4grp_high_8_8 > 17      6grp_total > 42      4grp_total > 38      6grp_cgc > 90      6grp_high1_sub > 02_03_04_05      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 140.5
(3)
P 		122.6
(0.6)
P 		134.2
(2.2)
P 		95.9
(-3)
A 		45
B 169.6
(3)
P 		116.6
(-0.5)
P 		82.2
(-2.8)
A 		79.7
(-3)
P 		90
C 150.9
(0.6)
P 		124.2
(-0.9)
P 		86.8
(-3)
P 		193.9
(3)
P 		107
avr 153.7
(3) 		121.1
(-0.7) 		101.1
(-3) 		123.2
(-0.5) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 92.8
(0.7)
A 		78.5
(-1.5)
A 		108.4
(3)
P 		97.1
(1.3)
A 		68.4
(-3)
A 		91
(0.4)
A 		40
B 91.7
(1.2)
P 		82.1
(0)
A 		106.6
(3)
P 		105.1
(2.8)
P 		103
(2.6)
P 		57.1
(-3)
A 		50
C 87.3
(-0.6)
P 		121.6
(2.9)
P 		84.6
(-0.9)
A 		64.2
(-3)
A 		122.4
(3)
P 		75.6
(-1.8)
P 		58
avr 90.6
(0.8) 		94.1
(1.6) 		99.9
(3) 		88.8
(0.4) 		97.9
(2.5) 		74.6
(-3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 51.2
(3)
P 		26.6
(-1)
P 		35.1
(0.4)
P 		14
(-3)
A 		37
B 54.1
(3)
P 		28.8
(-1.1)
P 		19.1
(-2.7)
A 		17
(-3)
P 		37
C 50
(3)
P 		35.7
(-1.3)
P 		30.2
(-3)
P 		32.7
(-2.2)
P 		20
avr 51.8
(3) 		30.4
(-1.2) 		28.1
(-1.6) 		21.2
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 17.1
(-3)
A 		27.9
(3)
A 		23.6
(0.6)
P 		24.6
(1.2)
A 		20.6
(-1.1)
A 		22.2
(-0.2)
A 		11
B 31.9
(0.1)
P 		27.3
(-1.2)
A 		25.9
(-1.7)
P 		27.8
(-1.1)
P 		41.8
(3)
P 		21.3
(-3)
A 		21
C 32.4
(1.4)
P 		37.1
(2.4)
P 		21.5
(-1.1)
A 		12.9
(-3)
A 		39.6
(3)
P 		25.3
(-0.2)
P 		27
avr 27.1
(-0.4) 		30.8
(1.4) 		23.7
(-2.1) 		21.8
(-3) 		34
(3) 		22.9
(-2.5) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188238_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.746
Sequence Type Exemplar sequence
Representative Public ID F16D3.1 NCBI
Target Description F16D3.1 /REP_DB=WormBase Gene ID /WP=CE09434 /TR=Q19490 /GB=CAB01495.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=tubulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059867(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059867 NCBI Caenorhabditis elegans F16D3.1 (tubulin) mRNA, complete cds. 11/11 None
GENEFINDER00000036933 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8361517:8365953:1 11/11 None
SNAP00000036925 ENSEMBL cdna:SNAP chromosome:CEL140:I:8364024:8365953:1 11/11 None
F16D3.1 ENSEMBL cdna:known chromosome:CEL140:I:8361535:8365953:1 gene:F16D3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8361465-8365884(+) 100.0 100.0
Public Domain and Genome References
Gene Title tubulin
Gene Symbol tba-5
Ensembl F16D3.1
Entrez Gene 184569 Entrez gene
SwissProt Q19490 EMBL-EBI
WormBase CE09434 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16458_S_AT tubulin alpha-3/alpha-5 chain (TUA3) at
ATH1-121501:245915_S_AT tubulin alpha-3/alpha-5 chain (TUA3) at
CANINE:1604133_S_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
CANINE:1594580_S_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
CANINE:1584444_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
CANINE_2:CFA.1372.1.S1_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
CANINE_2:CFAAFFX.13695.1.S1_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
CANINE_2:CFAAFFX.26512.1.S1_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
HG-U133_PLUS_2:213646_X_AT tubulin, alpha, ubiquitous hs
HG-U133A_2:213646_X_AT tubulin, alpha, ubiquitous hs
HG-U133A:213646_X_AT tubulin, alpha, ubiquitous hs
HG-U133_PLUS_2:211058_X_AT tubulin, alpha, ubiquitous hs
HG-U133A:211058_X_AT tubulin, alpha, ubiquitous hs
HG-U133A_2:211058_X_AT tubulin, alpha, ubiquitous hs
HG-U133A:201090_X_AT tubulin, alpha, ubiquitous hs
HG-U133_PLUS_2:201090_X_AT tubulin, alpha, ubiquitous hs
HG-U133A_2:201090_X_AT tubulin, alpha, ubiquitous hs
HG-U133_PLUS_2:211072_X_AT tubulin, alpha, ubiquitous hs
HG-U133A:211072_X_AT tubulin, alpha, ubiquitous hs
HG-U133A_2:211072_X_AT tubulin, alpha, ubiquitous hs
U133_X3P:G5174476_3P_X_AT tubulin, alpha, ubiquitous hs
U133_X3P:G13623540_3P_X_AT tubulin, alpha, ubiquitous hs
U133_X3P:HS2.382109.1.A1_3P_AT tubulin, alpha, ubiquitous hs
U133_X3P:HS.272897.2.S1_3P_X_AT tubulin, alpha, ubiquitous hs
U133_X3P:HS.240615.2.S1_3P_X_AT tubulin, alpha, ubiquitous hs
HG-U133_PLUS_2:1565668_AT tubulin, alpha, ubiquitous hs
HG-U133A_2:212639_X_AT tubulin, alpha, ubiquitous hs
HG-U133_PLUS_2:212639_X_AT tubulin, alpha, ubiquitous hs
HG-U133A:212639_X_AT tubulin, alpha, ubiquitous hs
HG-U95AV2:32272_AT tubulin, alpha, ubiquitous hs
MU11KSUBB:MSA.13449.0_AT tubulin, alpha 2 mm
MU11KSUBB:MSA.11390.0_AT tubulin, alpha 2 mm
MU11KSUBB:MSA.10568.0_F_AT tubulin, alpha 2 mm
MG-U74AV2:98759_F_AT tubulin, alpha 2 mm
MOE430A:1423846_X_AT tubulin, alpha 2 mm
MOUSE430A_2:1423846_X_AT tubulin, alpha 2 mm
MOUSE430_2:1423846_X_AT tubulin, alpha 2 mm
MOE430A:1433584_AT tubulin, alpha 2 mm
MOUSE430A_2:1433584_AT tubulin, alpha 2 mm
MOUSE430_2:1433584_AT tubulin, alpha 2 mm
MU19KSUBA:TC22648_F_AT Tubulin, alpha 2, mRNA (cDNA clone MGC:70226 IMAGE:4458530) mm
MU19KSUBC:TC35905_AT Tubulin, alpha 2, mRNA (cDNA clone MGC:70226 IMAGE:4458530) mm
MU19KSUBC:TC35911_AT Alpha-tubulin gene M-alpha-2, 3' end mm
MU19KSUBC:TC35912_F_AT Tubulin, alpha 2, mRNA (cDNA clone MGC:7731 IMAGE:3498312) mm
RG-U34A:J00797CDS_S_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) rn
RG-U34A:RC_AI169370_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) rn
RAE230A:1367579_A_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) rn
RAT230_2:1367579_A_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01495 Hypothetical protein F16D3.1 [Caenorhabditis elegans] ref|NP_492268.1| F16D3.1 [Caenorhabditis elegans] 0.0
blast CAE60464 Hypothetical protein CBG04075 [Caenorhabditis briggsae] 0.0
blast 1Z2B hain C, Tubulin-Colchicine-Vinblastine: Stathmin-Like Domain Complex pdb|1Z2B|A Chain A, Tubulin-Colchicine-Vinblastine: Stathmin-Like Domain Complex 0.0
blast CAB01495 Hypothetical protein F16D3.1 [Caenorhabditis elegans] ref|NP_492268.1| F16D3.1 [Caenorhabditis elegans] 1.0E-150
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 5.3E-101
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.5E-75
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 5.3E-101
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.5E-75
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 4.7E-30
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.5E-75
Sequence
Target Sequence 
>C. ELEGANS:188238_AT
actgcttcacttcgtttcgatggagcgttgaatgttgatttgacagagtttcagactaat
ttggttccatatccccgtattcactttccacttgtcacatatgctccaatcatttcggca
gaaaaagcatatcacgaacaactgtcggtgtctgaaatcacaaatgtgtgctttgaacca
ggaagtcaaatggtgaagtgtgatccaagaaatggaaagtatatggcatgttgccttttg
tatcgtggagatgttgtaccaaaagaaattaattctgcaatttcaacaatcaaaactaaa
agagcaattcaatttgtggattggtgtccaactggttttaaagttggcatcaattatcaa
ccaccgactgttgttccaggtggtgatatggcaaagcttcaacgtgccgtgtgcatgttg
tcaaatac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTGCTTCACTTCGTTTCGATGGAG 141 99 727 Antisense
GACTAATTTGGTTCCATATCCCCGT 241 373 780 Antisense
TTCACTTTCCACTTGTCACATATGC 449 687 806 Antisense
TTGTCACATATGCTCCAATCATTTC 593 707 818 Antisense
TCACGAACAACTGTCGGTGTCTGAA 231 627 858 Antisense
ATGTGTGCTTTGAACCAGGAAGTCA 613 49 890 Antisense
GGCATGTTGCCTTTTGTATCGTGGA 288 537 951 Antisense
GCATCAATTATCAACCACCGACTGT 520 307 1073 Antisense
ACCGACTGTTGTTCCAGGTGGTGAT 347 89 1089 Antisense
GCTTCAACGTGCCGTGTGCATGTTG 122 305 1122 Antisense
GTGCCGTGTGCATGTTGTCAAATAC 190 479 1130 Antisense
Sequence Source Affymetrix Proprietary Database