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[188237_at]

Description Y56A3A.13 /REP_DB=WormBase Gene ID /WP=CE22580 /GEN=nft-1 /TR=O76463 /GB=CAB60517.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 84      4grp_highest_8_8 > 13      4grp_high_8_8 > 31      6grp_highest_nonorm > 75      6grp_total > 74      4grp_total > 24      6grp_cgc > 66      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 493
1 2 3 4 range
A 609.2
(-3)
P 		742.9
(-1.3)
P 		824.1
(-0.3)
P 		1081.3
(3)
P 		472
B 760.1
(-1.7)
P 		728.9
(-3)
P 		803.3
(0)
P 		875.7
(3)
P 		147
C 588.4
(-3)
P 		826
(1.8)
P 		888
(3)
P 		832.3
(1.9)
P 		300
avr 652.6
(-3) 		765.9
(-0.5) 		838.5
(1) 		929.8
(3) 		277

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 480
1 2 3 4 5 6 range
A 873.4
(-1)
P 		950
(0.5)
P 		772.9
(-3)
P 		1075.7
(3)
P 		822.5
(-2)
P 		1019.9
(1.9)
P 		303
B 684.1
(-3)
P 		788.6
(-0.8)
P 		860
(0.7)
P 		848
(0.4)
P 		918.1
(1.9)
P 		972.1
(3)
P 		288
C 787.5
(-3)
P 		859.2
(-1.9)
P 		1163.6
(3)
P 		926.5
(-0.8)
P 		958.6
(-0.3)
P 		928.5
(-0.8)
P 		376
avr 781.7
(-3) 		865.9
(-0.4) 		932.2
(1.7) 		950.1
(2.3) 		899.7
(0.7) 		973.5
(3) 		192
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 221.8
(3)
P 		161.3
(-2.7)
P 		215.4
(2.4)
P 		158.3
(-3)
P 		64
B 242.4
(3)
P 		180
(-3)
P 		186.1
(-2.4)
P 		186.6
(-2.4)
P 		62
C 194.8
(-1.1)
P 		237.5
(0.4)
P 		309.5
(3)
P 		140.3
(-3)
P 		169
avr 219.7
(1.6) 		192.9
(-0.5) 		237
(3) 		161.7
(-3) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 212
1 2 3 4 5 6 range
A 161
(-3)
P 		337.9
(3)
P 		168.3
(-2.8)
P 		272.8
(0.8)
P 		248.2
(-0)
P 		248.3
(-0)
P 		177
B 238
(-1.9)
P 		262.5
(-1)
P 		208.8
(-3)
P 		224.6
(-2.4)
P 		373
(3)
P 		362.5
(2.6)
P 		164
C 291.9
(2.1)
P 		262.2
(0.7)
P 		296.1
(2.3)
P 		186.3
(-3)
P 		310.2
(3)
P 		310.8
(3)
P 		125
avr 230.3
(-2.6) 		287.5
(1.4) 		224.4
(-3) 		227.9
(-2.8) 		310.5
(3) 		307.2
(2.8) 		86
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188237_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6420
Sequence Type Exemplar sequence
Representative Public ID Y56A3A.13 NCBI
Target Description Y56A3A.13 /REP_DB=WormBase Gene ID /WP=CE22580 /GEN=nft-1 /TR=O76463 /GB=CAB60517.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067155(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067155 NCBI Caenorhabditis elegans NitFhit family member (nft-1) (nft-1) mRNA, complete cds. 11/11 None
SNAP00000035202 ENSEMBL cdna:SNAP chromosome:CEL140:III:11923766:11932948:1 11/11 None
GENEFINDER00000035234 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11928540:11930436:1 10/11 None
Y56A3A.13 ENSEMBL cdna:known chromosome:CEL140:III:11923762:11929255:1 gene:Y56A3A.13 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000035226 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11923761-11929205(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nft-1
Ensembl Y56A3A.13
Entrez Gene 176628 Entrez gene
SwissProt O76463 EMBL-EBI
WormBase CE22580 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1626557_AT Nitrilase and fragile histidine triad fusion protein dm
DROSGENOME1:144036_AT Nitrilase and fragile histidine triad fusion protein dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6807 nitrogen compound metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60517 Hypothetical protein Y56A3A.13 [Caenorhabditis elegans] ref|NP_499556.1| NitFhit family member (nft-1) [Caenorhabditis elegans] sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusion protein NitFhit [Includes: Bis(5'-adenosyl)-triphosphatase (Diadenosine 5',5'''-P1,P3-triphosphate hydrolase) (Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase); Nitrilase homolog ] gb|AAC39136.1| nitrilase and fragile histidine triad fusion protein NitFhit [Caenorhabditis elegans] pdb|1EMS|B Chain B, Crystal Structure Of The C. Elegans Nitfhit Protein pdb|1EMS|A Chain A, Crystal Structure Of The C. Elegans Nitfhit Protein 0.0
blast CAE67748 Hypothetical protein CBG13323 [Caenorhabditis briggsae] 0.0
blast CAE67743 Hypothetical protein CBG13318 [Caenorhabditis briggsae] 0.0
blast CAB60517 Hypothetical protein Y56A3A.13 [Caenorhabditis elegans] ref|NP_499556.1| NitFhit family member (nft-1) [Caenorhabditis elegans] sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusion protein NitFhit [Includes: Bis(5'-adenosyl)-triphosphatase (Diadenosine 5',5'''-P1,P3-triphosphate hydrolase) (Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase); Nitrilase homolog ] gb|AAC39136.1| nitrilase and fragile histidine triad fusion protein NitFhit [Caenorhabditis elegans] pdb|1EMS|B Chain B, Crystal Structure Of The C. Elegans Nitfhit Protein pdb|1EMS|A Chain A, Crystal Structure Of The C. Elegans Nitfhit Protein 5.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR001310 EMBL-EBI Histidine triad (HIT) protein 1.3E-39
Pfam IPR003010 EMBL-EBI Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 3.2E-73
Pfam IPR001310 EMBL-EBI Histidine triad (HIT) protein 1.3E-39
Sequence
Target Sequence 
>C. ELEGANS:188237_AT
gcgctgttgttgcacagtgttccgagagagttgacatgtgctttgctgaaatcgacttga
gctacgtggacactcttcgtgaaatgcaaccagtattctcccacagacgctccgatctct
acacacttcacataaatgaaaaatcttctgaaactggtggcctgaaattcgcccgattca
atattccagctgatcatattttctacagtactccgcactcatttgtatttgtcaacttga
aaccagtcactgatggacacgttcttgtaagcccaaaacgagtagtcccaagattgaccg
acttgactgatgctgagactgccgacttgttcatcgtcgcgaaaaaggttcaagcaatgc
tggagaagcatcataatgtgacgtcgacgacgatttgtgtgcaagatgggaaggatgctg
ggcagactgttccacacgttcacattcatatccttccccgccgcgccggagactttggcg
ataacgaaatctaccaaaaacttgcgtcacatgataaggagcccgagcgaaagccacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGCTGTTGTTGCACAGTGTTCCGA 669 287 746 Antisense
TGAGCTACGTGGACACTCTTCGTGA 404 569 803 Antisense
GTGAAATGCAACCAGTATTCTCCCA 166 483 824 Antisense
CGATCTCTACACACTTCACATAAAT 615 247 858 Antisense
ACTGGTGGCCTGAAATTCGCCCGAT 214 97 898 Antisense
AGTACTCCGCACTCATTTGTATTTG 457 61 952 Antisense
GATGGACACGTTCTTGTAAGCCCAA 457 407 997 Antisense
GACCGACTTGACTGATGCTGAGACT 446 381 1041 Antisense
CTGAGACTGCCGACTTGTTCATCGT 357 239 1058 Antisense
TCCACACGTTCACATTCATATCCTT 555 587 1176 Antisense
ATAAGGAGCCCGAGCGAAAGCCACG 528 19 1259 Antisense
Sequence Source Affymetrix Proprietary Database