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[188234_s_at]

Description Y46G5A.30 /REP_DB=WormBase Gene ID /WP=CE24301 /GEN=snf-5 /TR=Q9U2E0 /GB=CAB60372.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 0      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 2.9
(-3)
A 		3.8
(-2.2)
A 		9.8
(3)
A 		5.4
(-0.8)
A 		7
B 3.4
(-2.9)
A 		4.3
(-2.6)
A 		3
(-3)
A 		23.5
(3)
A 		21
C 5.7
(0.1)
A 		3.3
(-3)
A 		3.4
(-2.9)
A 		8
(3)
A 		5
avr 4
(-2.9) 		3.8
(-3) 		5.4
(-1.9) 		12.3
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 10.5
(-2.6)
A 		2.5
(-3)
A 		2
(-3)
A 		22.7
(-2.1)
A 		79.5
(0.3)
A 		144.3
(3)
P 		142
B 2.6
(-3)
A 		2.5
(-3)
A 		2.1
(-3)
A 		2.8
(-3)
A 		59.5
(0.1)
A 		114.3
(3)
P 		112
C 2.5
(-3)
A 		2.9
(-3)
A 		10.6
(-2.5)
A 		4.3
(-2.9)
A 		99.6
(3)
P 		97.6
(2.9)
P 		97
avr 5.2
(-2.9) 		2.6
(-3) 		4.9
(-2.9) 		9.9
(-2.6) 		79.5
(1) 		118.7
(3) 		116
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 1
(-2.3)
A 		0.8
(-3)
A 		2.6
(3)
A 		0.8
(-3)
A 		2
B 1.1
(-2.4)
A 		1.1
(-2.4)
A 		0.7
(-3)
A 		5
(3)
A 		4
C 1.9
(3)
A 		1
(-3)
A 		1.2
(-1.7)
A 		1.3
(-1)
A 		1
avr 1.3
(-1.7) 		1
(-3) 		1.5
(-0.9) 		2.4
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 1.9
(-2.7)
A 		0.9
(-2.9)
A 		0.4
(-3)
A 		5.8
(-2.1)
A 		24
(1.1)
A 		35.1
(3)
P 		35
B 0.9
(-2.9)
A 		0.8
(-3)
A 		0.5
(-3)
A 		0.7
(-3)
A 		24.2
(0.4)
A 		42.6
(3)
P 		42
C 0.9
(-3)
A 		0.9
(-3)
A 		2.7
(-2.7)
A 		0.9
(-3)
A 		32.2
(2.9)
P 		32.7
(3)
P 		32
avr 1.2
(-2.9) 		0.9
(-3) 		1.2
(-2.9) 		2.5
(-2.7) 		26.8
(1.3) 		36.8
(3) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188234_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3294
Sequence Type Exemplar sequence
Representative Public ID Y46G5A.30 NCBI
Target Description Y46G5A.30 /REP_DB=WormBase Gene ID /WP=CE24301 /GEN=snf-5 /TR=Q9U2E0 /GB=CAB60372.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064334(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064334 NCBI Caenorhabditis elegans Sodium:Neurotransmitter symporter Family family member (snf-5) (snf-5) mRNA, complete cds. 11/11 A 
SNAP00000039014 ENSEMBL cdna:SNAP chromosome:CEL140:II:12861983:12865147:-1 11/11 None
GENEFINDER00000039029 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12861983:12865147:-1 11/11 None
Y46G5A.30 ENSEMBL cdna:known chromosome:CEL140:II:12861882:12865718:-1 gene:Y46G5A.30 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12861907-12864859(-) 99.81 99.81
Public Domain and Genome References
Gene Title
Gene Symbol snf-5
Ensembl Y46G5A.30
Entrez Gene 174923 Entrez gene
SwissProt Q9U2E0 EMBL-EBI
WormBase CE29609 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6836 neurotransmitter transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5887 integral to plasma membrane inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5328 neurotransmitter:sodium symporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60372 Hypothetical protein Y46G5A.30 [Caenorhabditis elegans] ref|NP_496735.1| Sodium:Neurotransmitter symporter Family family member (snf-5) [Caenorhabditis elegans] 0.0
blast CAE59375 Hypothetical protein CBG02728 [Caenorhabditis briggsae] 0.0
blast CAE57482 Hypothetical protein CBG00451 [Caenorhabditis briggsae] 1.0E-176
Protein Domains
Database ID Description E-Value
Pfam IPR000175 EMBL-EBI Sodium:neurotransmitter symporter 5.9E-26
Pfam IPR000175 EMBL-EBI Sodium:neurotransmitter symporter 7.7E-68
Pfam IPR000175 EMBL-EBI Sodium:neurotransmitter symporter 5.9E-26
Pfam IPR000175 EMBL-EBI Sodium:neurotransmitter symporter 7.7E-68
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496735.1 10 116-138,164-186,283-305,315-337,397-419,456-478,498-520,530-552,578-600,620-642
Y46G5A.30 10 116-138,164-186,283-305,315-337,397-419,456-478,498-520,530-552,578-600,620-642
SNAP00000039014 10 132-154,180-202,299-321,331-353,413-435,472-494,514-536,546-568,594-616,636-658
GENEFINDER00000039029 10 132-154,180-202,299-321,331-353,413-435,472-494,514-536,546-568,594-616,636-658
Sequence
Target Sequence 
>C. ELEGANS:188234_S_AT
acgagtatgctgctggcttctcgtcagtgtgcactgttgtctgtgagctactggtcatga
tgtacatctacggattccgcaacgttcgagacgatattacggaagttgttggtcacgcca
gaaacaagttcaccggtgctatcggagctcactcttggtattttaccgctaactggatgg
tgatctctccgagcattgcgctgattctcgttggtctctcgttcgtacgcgagtacccat
atatgggaagacatgatatctacccggctgtctttgatattttcggatggttcctctcgt
tc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGAGTATGCTGCTGGCTTCTCGTC 692 93 1550 Antisense
GTGTGCACTGTTGTCTGTGAGCTAC 681 487 1576 Antisense
TACGGATTCCGCAACGTTCGAGACG 374 647 1618 Antisense
AAGTTCACCGGTGCTATCGGAGCTC 258 157 1675 Antisense
GGAGCTCACTCTTGGTATTTTACCG 475 521 1693 Antisense
AACTGGATGGTGATCTCTCCGAGCA 277 143 1720 Antisense
TCTCCGAGCATTGCGCTGATTCTCG 244 611 1735 Antisense
TCTCTCGTTCGTACGCGAGTACCCA 570 611 1764 Antisense
GAAGACATGATATCTACCCGGCTGT 393 339 1796 Antisense
CTACCCGGCTGTCTTTGATATTTTC 451 225 1809 Antisense
TATTTTCGGATGGTTCCTCTCGTTC 195 661 1827 Antisense
Sequence Source Affymetrix Proprietary Database