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[188230_s_at]

Description Y50E8A.4 /REP_DB=WormBase Gene ID /WP=CE24405 /GEN=unc-61 /TR=Q9U277 /GB=CAB60583.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 87      6grp_highest_nonorm > 93      6grp_total > 77      4grp_total > 61      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 242
1 2 3 4 range
A 228.9
(1.9)
P 		257.3
(3)
P 		249.7
(2.7)
P 		101.9
(-3)
A 		155
B 331.7
(3)
P 		235.7
(0.6)
P 		167.9
(-1.1)
P 		89.4
(-3)
A 		242
C 268.1
(1.5)
P 		271
(1.6)
P 		308.8
(3)
P 		141.5
(-3)
A 		167
avr 276.2
(3) 		254.7
(2.2) 		242.1
(1.8) 		110.9
(-3) 		165

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 616
1 2 3 4 5 6 range
A 216.2
(-3)
P 		305.7
(-2)
P 		282.3
(-2.3)
P 		387.5
(-1.1)
P 		317.4
(-1.9)
P 		756.9
(3)
P 		541
B 184.8
(-3)
P 		188.4
(-3)
P 		229.9
(-2.5)
P 		233.3
(-2.4)
P 		395.5
(-0.5)
P 		683.9
(3)
P 		499
C 322.2
(-2.3)
P 		285
(-2.7)
P 		258.5
(-3)
P 		300.3
(-2.5)
P 		476.8
(-0.6)
P 		801.2
(3)
P 		543
avr 241.1
(-3) 		259.7
(-2.8) 		256.9
(-2.8) 		307
(-2.2) 		396.6
(-1.2) 		747.3
(3) 		506
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 83.4
(3)
P 		55.9
(0.6)
P 		65.3
(1.4)
P 		14.9
(-3)
A 		69
B 105.8
(3)
P 		58.2
(-0.3)
P 		38.9
(-1.6)
P 		19.1
(-3)
A 		87
C 88.7
(1.6)
P 		77.9
(0.9)
P 		107.6
(3)
P 		23.9
(-3)
A 		84
avr 92.6
(3) 		64
(0.7) 		70.6
(1.2) 		19.3
(-3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 228
1 2 3 4 5 6 range
A 39.9
(-3)
P 		108.7
(-0.1)
P 		61.5
(-2.1)
P 		98.3
(-0.6)
P 		95.8
(-0.7)
P 		184.3
(3)
P 		144
B 64.3
(-2.7)
P 		62.7
(-2.8)
P 		55.8
(-3)
P 		61.8
(-2.8)
P 		160.7
(0.2)
P 		255
(3)
P 		199
C 119.4
(-1.3)
P 		87
(-2.2)
P 		65.8
(-2.8)
P 		60.4
(-3)
P 		154.3
(-0.3)
P 		268.2
(3)
P 		208
avr 74.5
(-2.5) 		86.1
(-2.1) 		61
(-3) 		73.5
(-2.6) 		136.9
(-0.4) 		235.8
(3) 		175
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188230_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14507
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.4 NCBI
Target Description Y50E8A.4 /REP_DB=WormBase Gene ID /WP=CE24405 /GEN=unc-61 /TR=Q9U277 /GB=CAB60583.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074237(11), NM_182356(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074237 NCBI Caenorhabditis elegans UNCoordinated family member (unc-61) (unc-61) mRNA, complete cds. 11/11 None
NM_182356 NCBI Caenorhabditis elegans UNCoordinated family member (unc-61) (unc-61) mRNA, complete cds. 11/11 None
SNAP00000008979 ENSEMBL cdna:SNAP chromosome:CEL140:V:14734065:14736433:-1 11/11 None
GENEFINDER00000009983 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14734065:14736433:-1 11/11 None
Y50E8A.4b ENSEMBL cdna:known chromosome:CEL140:V:14733746:14736466:-1 gene:Y50E8A.4 11/11 A 
Y50E8A.4a ENSEMBL cdna:known chromosome:CEL140:V:14734065:14736826:-1 gene:Y50E8A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14734064-14736433(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y50E8A.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD54172 Hypothetical protein Y50E8A.4a [Caenorhabditis elegans] ref|NP_872156.1| UNCoordinated family member (unc-61) [Caenorhabditis elegans] 0.0
blast CAB60583 Hypothetical protein Y50E8A.4b [Caenorhabditis elegans] ref|NP_506638.1| UNCoordinated family member (unc-61) [Caenorhabditis elegans] 0.0
blast CAE60841 Hypothetical protein CBG04550 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000038 EMBL-EBI Cell division/GTP binding protein 7.9E-120
Pfam IPR000038 EMBL-EBI Cell division/GTP binding protein 7.9E-120
Pfam IPR000038 EMBL-EBI Cell division/GTP binding protein 7.9E-120
Pfam IPR000038 EMBL-EBI Cell division/GTP binding protein 4.8E-17
Pfam IPR000038 EMBL-EBI Cell division/GTP binding protein 1.6E-97
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_872156.1 1 7-29
Y50E8A.4a 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:188230_S_AT
cgatatttacacgttcccaactgacgatgaaactgtctcaacgacgaacaaggaaatgaa
taaatcggttccgttcgccgttgttggaagtattgattttgtgaagaaagagaatggaca
aatggttcgtgctcgtcaatatccatggggaatagtcgaagtggagaatgaatcacattg
tgattttgttaaactccgtgaagcacttctacgtacaaatgttgatgagatgagacaacg
aactcacgagtcactctatgaaaattaccgtcgtgacagacttcgtcagatgaagattgg
agatggagagactggaccaaagattattgaaaaactcgcacagaaacatcgcgagcatca
agacgagttcagccgtcgtgagcttactcttcgcgaagaatttcagaagaagcttgatgt
aacagaaggtgacatgagaaaagttgaagaaggattggctgcacgcgagcgagaggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGATATTTACACGTTCCCAACTGAC 436 247 813 Antisense
TGAATAAATCGGTTCCGTTCGCCGT 419 573 869 Antisense
TCCGTTCGCCGTTGTTGGAAGTATT 243 591 882 Antisense
GAATGGACAAATGGTTCGTGCTCGT 312 333 924 Antisense
TCGTGCTCGTCAATATCCATGGGGA 629 605 939 Antisense
AACTCCGTGAAGCACTTCTACGTAC 281 139 1004 Antisense
GACAACGAACTCACGAGTCACTCTA 394 363 1046 Antisense
AATTACCGTCGTGACAGACTTCGTC 48 175 1075 Antisense
AAGACGAGTTCAGCCGTCGTGAGCT 295 153 1172 Antisense
TCGTGAGCTTACTCTTCGCGAAGAA 199 601 1188 Antisense
GATTGGCTGCACGCGAGCGAGAGGT 563 433 1265 Antisense
Sequence Source Affymetrix Proprietary Database