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[188225_s_at]

Description Y60A3A.12 /REP_DB=WormBase Gene ID /WP=CE27297 /GEN=chk-2 /TR=Q9U1Y5 /GB=CAB60407.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 43      4grp_total > 10      6grp_cgc > 91      6grp_cgc_sub > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 21.1
(-1.9)
A 		24.3
(-1.7)
A 		7.5
(-3)
A 		82.8
(3)
A 		75
B 3.2
(-3)
A 		12.1
(-2.1)
A 		7
(-2.6)
A 		66
(3)
A 		63
C 10.9
(-2.5)
A 		15.3
(-2.3)
A 		3.9
(-3)
A 		96.1
(3)
A 		92
avr 11.7
(-2.6) 		17.2
(-2.1) 		6.1
(-3) 		81.6
(3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 5.5
(-2.5)
A 		7.8
(-2)
A 		27.9
(3)
A 		21.9
(1.5)
A 		6.2
(-2.4)
A 		3.6
(-3)
A 		24
B 16.8
(2)
A 		14.7
(1.3)
A 		19.5
(3)
A 		4
(-2.6)
A 		4.5
(-2.5)
A 		3
(-3)
A 		17
C 14.8
(-0.8)
A 		8.2
(-2.2)
A 		33.2
(3)
A 		4.2
(-3)
A 		5
(-2.8)
A 		10.2
(-1.8)
A 		29
avr 12.4
(-1) 		10.2
(-1.6) 		26.9
(3) 		10
(-1.7) 		5.2
(-3) 		5.6
(-2.9) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 7.7
(0.4)
A 		5.3
(-1)
A 		1.9
(-3)
A 		12.1
(3)
A 		10
B 1
(-3)
A 		3
(-2.1)
A 		1.6
(-2.7)
A 		14.1
(3)
A 		13
C 3.6
(-2.1)
A 		4.4
(-1.8)
A 		1.4
(-3)
A 		16.2
(3)
A 		15
avr 4.1
(-1.8) 		4.2
(-1.8) 		1.6
(-3) 		14.1
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1
(-2.9)
A 		2.8
(-0.8)
A 		6.1
(3)
A 		5.6
(2.4)
A 		1.9
(-1.8)
A 		0.9
(-3)
A 		5
B 5.8
(3)
A 		4.9
(1.9)
A 		4.7
(1.6)
A 		1.1
(-3)
A 		1.8
(-2.1)
A 		1.1
(-3)
A 		5
C 5.5
(0.7)
A 		2.5
(-1.7)
A 		8.4
(3)
A 		0.8
(-3)
A 		1.6
(-2.4)
A 		3.4
(-0.9)
A 		8
avr 4.1
(-0) 		3.4
(-0.9) 		6.4
(3) 		2.5
(-2.1) 		1.8
(-3) 		1.8
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188225_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12961
Sequence Type Exemplar sequence
Representative Public ID Y60A3A.12 NCBI
Target Description Y60A3A.12 /REP_DB=WormBase Gene ID /WP=CE27297 /GEN=chk-2 /TR=Q9U1Y5 /GB=CAB60407.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029100(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029100 NCBI Caenorhabditis elegans CHeckpoint Kinase family member (chk-2) (chk-2) mRNA, complete cds. 11/11 None
SNAP00000032795 ENSEMBL cdna:SNAP chromosome:CEL140:V:19902211:19920588:-1 9/11 None
GENEFINDER00000032821 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19906181:19914342:-1 10/11 None
Y60A3A.12 ENSEMBL cdna:known chromosome:CEL140:V:19906887:19913970:-1 gene:Y60A3A.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075659 5/11 Cross Hyb Matching Probes None
GENEFINDER00000032591 5/11 Cross Hyb Matching Probes None
SNAP00000032584 3/11 Cross Hyb Matching Probes None
T08D2.7 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19907831-19913839(-) 99.72 99.72
chrX:167731-175465(-) 93.64 93.64
Public Domain and Genome References
Gene Title
Gene Symbol chk-2
Ensembl Y60A3A.12
Entrez Gene 180304 Entrez gene
SwissProt Q9U1Y5 EMBL-EBI
WormBase CE27297 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18010.1.S1_S_AT similar to protein kinase CHK2 isoform a cfa
CANINE_2:CFAAFFX.18523.1.S1_S_AT similar to protein kinase CHK2 isoform a cfa
DROSOPHILA_2:1625038_S_AT loki dm
DROSGENOME1:154411_AT loki dm
HG-U133_PLUS_2:210416_S_AT CHK2 checkpoint homolog (S. pombe) hs
HG-U133A:210416_S_AT CHK2 checkpoint homolog (S. pombe) hs
HG-FOCUS:210416_S_AT CHK2 checkpoint homolog (S. pombe) hs
HG-U133A_2:210416_S_AT CHK2 checkpoint homolog (S. pombe) hs
U133_X3P:G13278893_3P_A_AT CHK2 checkpoint homolog (S. pombe) hs
HG-U95AV2:37887_AT CHK2 checkpoint homolog (S. pombe) hs
HG-U95D:79039_AT CHK2 checkpoint homolog (S. pombe) hs
MG-U74AV2:92481_AT CHK2 checkpoint homolog (S. pombe) mm
MOE430A:1422747_AT CHK2 checkpoint homolog (S. pombe) mm
MOUSE430A_2:1422747_AT CHK2 checkpoint homolog (S. pombe) mm
MOUSE430_2:1422747_AT CHK2 checkpoint homolog (S. pombe) mm
MU19KSUBB:TC30457_AT CHK2 checkpoint homolog (S. pombe) (Chek2), mRNA mm
RG-U34C:RC_AI071512_AT protein kinase Chk2 rn
RAE230A:1369010_AT protein kinase Chk2 rn
RAT230_2:1369010_AT protein kinase Chk2 rn
YEAST_2:1773949_AT Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60407 Hypothetical protein Y60A3A.12 [Caenorhabditis elegans] ref|NP_001024271.1| CHeckpoint Kinase family member (chk-2) [Caenorhabditis elegans] sp|Q9U1Y5|CHK2_CAEEL Serine/threonine-protein kinase chk-2 (Ce-cds-1) (Ce-chk-2) dbj|BAB20578.1| Ce-CDS-1 [Caenorhabditis elegans] 0.0
blast BAB15803.1 CeCHK2 [Caenorhabditis elegans] 0.0
blast CAB07420 Hypothetical protein T08D2.7 [Caenorhabditis elegans] ref|NP_508060.1| T08D2.7 [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 9.0E-61
hanks 2.1.18 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; MRE4 2.0E-73
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 2.0E-24
hanks 1.9.6 AGC group; AGC Other; SPK1 4.0E-25
Protein Domains
Database ID Description E-Value
Pfam IPR000253 EMBL-EBI Forkhead-associated (FHA) 2.2E-12
Pfam IPR000719 EMBL-EBI Protein kinase 1.8E-78
Pfam IPR000253 EMBL-EBI Forkhead-associated (FHA) 2.2E-12
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-22
Pfam IPR000719 EMBL-EBI Protein kinase 6.6E-33
Pfam IPR002319 EMBL-EBI Phenylalanyl-tRNA synthetase, class IIc 3.9E-59
Pfam IPR000253 EMBL-EBI Forkhead-associated (FHA) 2.2E-12
Pfam IPR000719 EMBL-EBI Protein kinase 0.12
Pfam IPR000719 EMBL-EBI Protein kinase 8.9E-47
Pfam IPR000719 EMBL-EBI Protein kinase 6.4E-15
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000032821 2 467-489,595-617
Sequence
Target Sequence 
>C. ELEGANS:188225_S_AT
ggtctcggtgaatcgctcggaaaatatttcgcttttcagctaatcgacgcgattttgtat
cttcactctgttggaatctgtcatcgtgacattaaacccgaaaatattctgtgctcggat
aaagcggagcggtgcattttaaagctaaccgactttggaatggcgaaaaattcggtgaat
cgaatgaaaactcgctgtggaacgccgtcttataatgcgccggaaattgtggcgaatgag
ggtgttgagtacacgccaaaagtcgatatttggagcctcggatgcgtacttttcatcaca
ttttccggttatccaccgttcagcgaagaatatacggatatgacgatggatgaacaagtt
ttgacaggccgcctaatcttccacgcacaatggcgtcgaattactgtcgaaacacagaat
atgatcaaatggatgcttactgtcgagccatcaaaccgcccgtctgccgtcgaactaatg
agtactcaatggatgaaatgtgcggattgtcgtactgcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCTCGGTGAATCGCTCGGAAAAT 452 489 811 Antisense
ACGCGATTTTGTATCTTCACTCTGT 334 91 857 Antisense
TGAAAACTCGCTGTGGAACGCCGTC 534 573 995 Antisense
GAACGCCGTCTTATAATGCGCCGGA 350 347 1010 Antisense
ATTTGGAGCCTCGGATGCGTACTTT 555 17 1078 Antisense
TTCATCACATTTTCCGGTTATCCAC 678 685 1102 Antisense
GGTTATCCACCGTTCAGCGAAGAAT 101 505 1117 Antisense
GCCTAATCTTCCACGCACAATGGCG 25 279 1181 Antisense
GCTTACTGTCGAGCCATCAAACCGC 295 305 1245 Antisense
CCCGTCTGCCGTCGAACTAATGAGT 71 259 1269 Antisense
AAATGTGCGGATTGTCGTACTGCGA 114 121 1306 Antisense
Sequence Source Affymetrix Proprietary Database