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[188221_at]

Description F47G6.4 /REP_DB=WormBase Gene ID /WP=CE17841 /GEN=spe-15 /TR=Q9TZI9 /GB=AAK18955.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 79      6grp_highest_nonorm > 81      6grp_total > 79      4grp_total > 23      6grp_highest_sub > Cu      6grp_cgc > 29      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 224.1
(2.8)
P 		117.9
(-3)
A 		175
(0.1)
M 		227
(3)
P 		109
B 192.7
(1.8)
P 		206
(3)
A 		141.4
(-3)
P 		191.2
(1.6)
P 		65
C 216.8
(3)
P 		159.1
(-3)
A 		179.8
(-0.8)
A 		171.7
(-1.7)
P 		58
avr 211.2
(3) 		161
(-3) 		165.4
(-2.5) 		196.6
(1.3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 241
1 2 3 4 5 6 range
A 216.8
(-1)
P 		253.9
(-0)
P 		143
(-3)
P 		173.5
(-2.2)
P 		231.3
(-0.6)
P 		366.7
(3)
P 		224
B 257.9
(-1.2)
P 		282.9
(-0.3)
P 		201.6
(-3)
P 		220.4
(-2.4)
P 		302.5
(0.3)
P 		384.4
(3)
P 		183
C 213.6
(-2.8)
P 		237
(-1.7)
P 		210.4
(-3)
P 		270
(-0.2)
P 		306.4
(1.6)
P 		336.5
(3)
P 		126
avr 229.4
(-1.5) 		257.9
(-0.5) 		185
(-3) 		221.3
(-1.8) 		280.1
(0.2) 		362.5
(3) 		178
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 81.6
(3)
P 		25.6
(-3)
A 		45.8
(-0.8)
M 		33.2
(-2.2)
P 		56
B 61.5
(3)
P 		50.9
(0.8)
A 		32.8
(-3)
P 		40.7
(-1.3)
P 		29
C 71.8
(3)
P 		45.7
(-0.7)
A 		62.7
(1.7)
A 		29
(-3)
P 		43
avr 71.6
(3) 		40.7
(-2) 		47.1
(-0.9) 		34.3
(-3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 40
(-2.1)
P 		90.3
(3)
P 		31.1
(-3)
P 		44
(-1.7)
P 		69.8
(0.9)
P 		89.3
(2.9)
P 		59
B 89.8
(-0.4)
P 		94.2
(-0.1)
P 		49
(-3)
P 		58.4
(-2.4)
P 		122.9
(1.7)
P 		143.3
(3)
P 		94
C 79.2
(-0.4)
P 		72.3
(-1.1)
P 		53.5
(-3)
P 		54.3
(-2.9)
P 		99.1
(1.6)
P 		112.6
(3)
P 		59
avr 69.7
(-0.9) 		85.6
(0.5) 		44.5
(-3) 		52.2
(-2.3) 		97.3
(1.5) 		115.1
(3) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188221_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.140
Sequence Type Exemplar sequence
Representative Public ID F47G6.4 NCBI
Target Description F47G6.4 /REP_DB=WormBase Gene ID /WP=CE17841 /GEN=spe-15 /TR=Q9TZI9 /GB=AAK18955.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058455(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058455 NCBI Caenorhabditis elegans defective SPErmatogenesis family member (spe-15) (spe-15) mRNA, complete cds. 11/11 None
GENEFINDER00000020136 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1460279:1465215:1 11/11 None
SNAP00000020128 ENSEMBL cdna:SNAP chromosome:CEL140:I:1460558:1465215:1 11/11 None
F47G6.4 ENSEMBL cdna:known chromosome:CEL140:I:1446667:1465341:1 gene:F47G6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1446666-1465215(+) 99.89 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spe-15
Ensembl F47G6.4
Entrez Gene 171712 Entrez gene
SwissProt Q9TZI9 EMBL-EBI
WormBase CE17841 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.5028.1.S1_S_AT similar to Myosin-6 (Myosin VI) cfa
CANINE_2:CFAAFFX.5040.1.S1_AT similar to Myosin-6 (Myosin VI) cfa
DROSOPHILA_2:1623185_S_AT jaguar dm
DROSGENOME1:143639_AT jaguar dm
CHICKEN:GGA.3147.1.S1_AT myosin VI gga
HG-U133_PLUS_2:210480_S_AT myosin VI hs
HG-U133A:210480_S_AT myosin VI hs
HG-U133A_2:210480_S_AT myosin VI hs
HG-U133_PLUS_2:203216_S_AT myosin VI hs
HG-U133A_2:203216_S_AT myosin VI hs
HG-U133A:203216_S_AT myosin VI hs
HG-FOCUS:203216_S_AT myosin VI hs
HU35KSUBA:AB002387_AT myosin VI hs
HU35KSUBC:RC_W59961_S_AT myosin VI hs
HU35KSUBC:RC_AA160890_S_AT myosin VI hs
U133_X3P:G9280815_3P_A_AT myosin VI hs
U133_X3P:G4826845_3P_A_AT myosin VI hs
U133_X3P:HS.22564.0.S1_3P_A_AT myosin VI hs
HG-U133A_2:203215_S_AT myosin VI hs
HG-U133_PLUS_2:203215_S_AT myosin VI hs
HG-U133A:203215_S_AT myosin VI hs
HG-U95AV2:33375_AT myosin VI hs
HG-U95C:53545_AT Myosin VI hs
HU35KSUBD:RC_N64144_AT Myosin VI hs
MOUSE430_2:1421120_AT myosin VI mm
MOE430A:1421120_AT myosin VI mm
MOUSE430A_2:1421120_AT myosin VI mm
MU11KSUBA:AA648027_AT myosin VI mm
MU11KSUBA:U49739_S_AT myosin VI mm
MG-U74AV2:96560_AT myosin VI mm
MG-U74AV2:92382_AT myosin VI mm
MG-U74BV2:116837_AT myosin VI mm
MG-U74AV2:162245_F_AT myosin VI mm
MOE430A:1433942_AT myosin VI mm
MOUSE430A_2:1433942_AT myosin VI mm
MOUSE430_2:1433942_AT myosin VI mm
MOE430A:1435559_AT myosin VI mm
MOUSE430A_2:1435559_AT myosin VI mm
MOUSE430_2:1435559_AT myosin VI mm
RG-U34B:RC_AI013854_AT similar to Myosin VI rn
RAE230A:1376804_AT similar to Myosin VI rn
RAT230_2:1376804_AT similar to Myosin VI rn
RAE230A:1390277_AT Similar to Myosin VI rn
RAT230_2:1390277_AT Similar to Myosin VI rn
RG-U34B:RC_AA945910_AT Similar to Myosin VI rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16459 myosin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK18955 Defective spermatogenesis protein 15 [Caenorhabditis elegans] ref|NP_490856.1| defective SPErmatogenesis family member (spe-15) [Caenorhabditis elegans] 0.0
blast CAE74529 Hypothetical protein CBG22283 [Caenorhabditis briggsae] 0.0
blast AAA97927 hum-3 [Caenorhabditis elegans] 0.0
blast AAK18955 Defective spermatogenesis protein 15 [Caenorhabditis elegans] ref|NP_490856.1| defective SPErmatogenesis family member (spe-15) [Caenorhabditis elegans] 1.0E-163
blast AAK18955 Defective spermatogenesis protein 15 [Caenorhabditis elegans] ref|NP_490856.1| defective SPErmatogenesis family member (spe-15) [Caenorhabditis elegans] 1.0E-161
blast CAE74529 Hypothetical protein CBG22283 [Caenorhabditis briggsae] 1.0E-143
blast CAE74529 Hypothetical protein CBG22283 [Caenorhabditis briggsae] 1.0E-141
Protein Domains
Database ID Description E-Value
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 7.8E-14
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Pfam IPR000048 EMBL-EBI IQ calmodulin-binding region 1.0E-6
Sequence
Target Sequence 
>C. ELEGANS:188221_AT
atcttctgatggtgttgctcttgttgctcaggaagcatctgctgctccaccaaaacaaca
gtcgaagtatgatttgggtaactggaagtatgccgatcttcgtgatgctattaatacgag
taatgacatggagctcctggtagcctgcaaggaggaattccatcgtcgtcttcgcattta
caacgagtggaagtcaaagaactcggcgaatcgggatgttccaccgacacgtgctccatt
gacggtttataatcaacagaagccaagcagctcttcaatgatggtgtctcgggttcaagc
tgctcctcatctcaatccagcattgactcagcaacgttatttcaaagtgtcattcgctac
ggataacaagaagaaaaatggcggttctcaatctggaatgtggtatgctcacttcaatgg
tcaatacattcgcagacaactcacaattcgtccgtcgcaaaaaccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCTTCTGATGGTGTTGCTCTTGTT 438 31 3048 Antisense
GTTGCTCAGGAAGCATCTGCTGCTC 699 439 3070 Antisense
GTATGCCGATCTTCGTGATGCTATT 586 455 3135 Antisense
TGACATGGAGCTCCTGGTAGCCTGC 490 571 3171 Antisense
CAAGGAGGAATTCCATCGTCGTCTT 503 183 3195 Antisense
CGAATCGGGATGTTCCACCGACACG 567 253 3254 Antisense
GACACGTGCTCCATTGACGGTTTAT 680 367 3273 Antisense
GCAGCTCTTCAATGATGGTGTCTCG 665 313 3314 Antisense
AATCCAGCATTGACTCAGCAACGTT 186 167 3361 Antisense
TGGTATGCTCACTTCAATGGTCAAT 313 551 3448 Antisense
CACAATTCGTCCGTCGCAAAAACCG 598 191 3489 Antisense
Sequence Source Affymetrix Proprietary Database