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[188219_at]

Description F33D4.3 /REP_DB=WormBase Gene ID /WP=CE17033 /GEN=flp-13 /TR=O44185 /GB=AAB88376.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 12      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 32      6grp_total > 12      4grp_total > 22      6grp_highest_sub > 02      6grp_cgc > 34      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 958
1 2 3 4 range
A 1552.2
(3)
P 		809.5
(-2.3)
P 		714.2
(-3)
P 		1381.6
(1.8)
P 		838
B 1297.7
(1.6)
P 		883.7
(-1.6)
P 		703.3
(-3)
P 		1474.1
(3)
P 		771
C 1459.5
(2.3)
P 		989.8
(-0.6)
P 		609.7
(-3)
P 		1567.6
(3)
P 		958
avr 1436.5
(2.7) 		894.3
(-1.4) 		675.7
(-3) 		1474.4
(3) 		799

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1177
1 2 3 4 5 6 range
A 957.2
(-0.6)
P 		1232.2
(3)
P 		1113.1
(1.5)
P 		954.7
(-0.6)
P 		768.1
(-3)
P 		920.8
(-1)
P 		464
B 1454.6
(1.4)
P 		1620.1
(2.2)
P 		1773.6
(3)
P 		879.1
(-1.6)
P 		596.8
(-3)
P 		849.2
(-1.7)
P 		1177
C 1196.6
(0.1)
P 		1563.9
(3)
P 		984.4
(-1.5)
P 		1109.9
(-0.5)
P 		793.3
(-3)
P 		805.7
(-2.9)
P 		771
avr 1202.8
(0.9) 		1472.1
(3) 		1290.4
(1.6) 		981.2
(-0.9) 		719.4
(-3) 		858.6
(-1.9) 		753
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 402
1 2 3 4 range
A 565.2
(3)
P 		175.7
(-3)
P 		186.7
(-2.8)
P 		202.2
(-2.6)
P 		390
B 413.8
(3)
P 		218.3
(-1.7)
P 		163
(-3)
P 		314.1
(0.6)
P 		251
C 483.1
(3)
P 		284.6
(-1.4)
P 		212.5
(-3)
P 		264.3
(-1.9)
P 		271
avr 487.4
(3) 		226.2
(-2.2) 		187.4
(-3) 		260.2
(-1.5) 		300

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 363
1 2 3 4 5 6 range
A 176.5
(-3)
P 		438.2
(3)
P 		242.4
(-1.5)
P 		242.1
(-1.5)
P 		231.8
(-1.7)
P 		224.2
(-1.9)
P 		262
B 506.1
(2.3)
P 		539.3
(3)
P 		430.7
(0.9)
P 		232.9
(-3)
P 		242.5
(-2.8)
P 		316.7
(-1.4)
P 		306
C 443.5
(2.2)
P 		477.2
(3)
P 		250.6
(-2.4)
P 		223.2
(-3)
P 		256.7
(-2.2)
P 		269.7
(-1.9)
P 		254
avr 375.4
(0.4) 		484.9
(3) 		307.9
(-1.2) 		232.7
(-3) 		243.7
(-2.7) 		270.2
(-2.1) 		252
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188219_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9110
Sequence Type Exemplar sequence
Representative Public ID F33D4.3 NCBI
Target Description F33D4.3 /REP_DB=WormBase Gene ID /WP=CE17033 /GEN=flp-13 /TR=O44185 /GB=AAB88376.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068854(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068854 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-13) (flp-13) mRNA, complete cds. 11/11 None
GENEFINDER00000032737 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7697143:7698352:-1 11/11 None
F33D4.3 ENSEMBL cdna:known chromosome:CEL140:IV:7697143:7698352:-1 gene:F33D4.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032722 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7697148-7698358(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol flp-13
Ensembl F33D4.3
Entrez Gene 177547 Entrez gene
SwissProt O44185 EMBL-EBI
WormBase CE17033 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7218 neuropeptide signaling pathway inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB88376 Fmrf-like peptide protein 13 [Caenorhabditis elegans] ref|NP_501255.1| FMRF-Like Peptide family member (flp-13) [Caenorhabditis elegans] sp|O44185|FLP13_CAEEL FMRFamide-like neuropeptide 13 precursor [Contains: AADGAPLIRF-amide 1; AADGAPLIRF-amide 2] gb|AAC08951.1| FMRFamide-like peptide 13 [Caenorhabditis elegans] 5.0E-85
blast CAE61941 Hypothetical protein CBG05939 [Caenorhabditis briggsae] 2.0E-76
blast AAR19420 FMRFamide-like peptide precursor [Periplaneta americana] 2.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.063
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.51
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.017
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.51
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.017
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.11
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.59
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.17
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.23
Sequence
Target Sequence 
>C. ELEGANS:188219_AT
gctcactatctcaatgttcgtcgtcgccattcaggcctttgattcgtcggaaattcgaat
gctcgatgaacaatatgacacgaagaatcccttctttcaatttctcgaaaactcaaaacg
aagtgatcgaccgactcgtgcaatggattctccactgatccgttttggcaaaagagctgc
tgatggagcaccactcatcagatttggtcgtgctccagaagcatctccgttcattcgttt
tggaaagagagctgccgatggggctcctttgatcagatttggccgtgctccagaagcttc
tccattcatcagatttggcaaaagagcatctccatcagctccattgatccgtttcggacg
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCACTATCTCAATGTTCGTCGTC 391 301 27 Antisense
CGTCGCCATTCAGGCCTTTGATTCG 703 245 48 Antisense
GAAGAATCCCTTCTTTCAATTTCTC 8 339 108 Antisense
ACGAAGTGATCGACCGACTCGTGCA 208 91 144 Antisense
CGACTCGTGCAATGGATTCTCCACT 539 249 158 Antisense
GGAGCACCACTCATCAGATTTGGTC 303 521 211 Antisense
ATCTCCGTTCATTCGTTTTGGAAAG 349 33 249 Antisense
AAGAGAGCTGCCGATGGGGCTCCTT 41 155 271 Antisense
TGGGGCTCCTTTGATCAGATTTGGC 182 553 285 Antisense
GGCAAAAGAGCATCTCCATCAGCTC 141 535 343 Antisense
AGCTCCATTGATCCGTTTCGGACGT 467 79 363 Antisense
Sequence Source Affymetrix Proprietary Database