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[188218_s_at]

Description F59E12.2 /REP_DB=WormBase Gene ID /WP=CE25925 /GEN=zyg-1 /TR=O01903 /GB=AAB54253.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 15      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 149
1 2 3 4 range
A 194.8
(3)
P 		63.4
(-3)
A 		127
(-0.1)
A 		167
(1.7)
A 		131
B 201.9
(3)
P 		127.7
(-3)
A 		160.1
(-0.4)
A 		191.5
(2.2)
P 		74
C 212.1
(3)
P 		116.8
(-3)
A 		142.8
(-1.4)
A 		137
(-1.7)
A 		95
avr 202.9
(3) 		102.6
(-3) 		143.3
(-0.6) 		165.2
(0.7) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 244
1 2 3 4 5 6 range
A 204.4
(0.2)
A 		133.1
(-3)
A 		152.9
(-2.1)
A 		176.4
(-1)
A 		258
(2.6)
A 		266
(3)
P 		133
B 150.7
(-3)
A 		184.9
(-1.4)
A 		197.6
(-0.8)
A 		166.6
(-2.3)
A 		217.8
(0.1)
P 		278.8
(3)
P 		128
C 130.4
(-2.4)
A 		170.1
(-1.5)
M 		107.7
(-3)
A 		267.4
(0.9)
A 		188.8
(-1)
A 		351.6
(3)
P 		244
avr 161.8
(-2.6) 		162.7
(-2.6) 		152.7
(-3) 		203.5
(-0.9) 		221.5
(-0.2) 		298.8
(3) 		146
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 70.9
(3)
P 		13.8
(-3)
A 		33.2
(-1)
A 		24.4
(-1.9)
A 		57
B 64.4
(3)
P 		31.5
(-3)
A 		37.1
(-2)
A 		40.8
(-1.3)
P 		33
C 70.2
(3)
P 		33.6
(-1.7)
A 		49.8
(0.4)
A 		23.1
(-3)
A 		47
avr 68.5
(3) 		26.3
(-3) 		40
(-1.1) 		29.4
(-2.6) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 37.7
(-2.4)
A 		47.4
(-1.1)
A 		33.3
(-3)
A 		44.8
(-1.5)
A 		77.9
(3)
A 		64.8
(1.2)
P 		45
B 52.4
(-2.2)
A 		61.5
(-1.3)
A 		48
(-2.6)
A 		44.1
(-3)
A 		88.5
(1.4)
P 		104
(3)
P 		60
C 48.3
(-1.6)
A 		51.9
(-1.4)
M 		27.4
(-3)
A 		53.8
(-1.2)
A 		61.1
(-0.8)
A 		117.7
(3)
P 		90
avr 46.1
(-2) 		53.6
(-1.2) 		36.2
(-3) 		47.6
(-1.8) 		75.8
(1) 		95.5
(3) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188218_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4446
Sequence Type Exemplar sequence
Representative Public ID F59E12.2 NCBI
Target Description F59E12.2 /REP_DB=WormBase Gene ID /WP=CE25925 /GEN=zyg-1 /TR=O01903 /GB=AAB54253.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062702(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062702 NCBI Caenorhabditis elegans ZYGote defective: embryonic lethal family member (zyg-1) (zyg-1) mRNA, complete cds. 11/11 A 
SNAP00000018073 ENSEMBL cdna:SNAP chromosome:CEL140:II:5653331:5654196:1 11/11 None
GENEFINDER00000018086 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5653304:5654196:1 11/11 None
F59E12.2.1 ENSEMBL cdna:known chromosome:CEL140:II:5649706:5654334:1 gene:F59E12.2 11/11 None
F59E12.2.2 ENSEMBL cdna:known chromosome:CEL140:II:5649717:5654391:1 gene:F59E12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5649411-5654194(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol zyg-1
Ensembl F59E12.2
Entrez Gene 173956 Entrez gene
SwissProt Q9GT24 EMBL-EBI
WormBase CE28571 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5813 centrosome inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB54253 Zygote defective: embryonic lethal protein 1 [Caenorhabditis elegans] ref|NP_495103.1| ZYGote defective: embryonic lethal family member (zyg-1) [Caenorhabditis elegans] gb|AAG15377.1| zygote defective-1 [Caenorhabditis elegans] sp|Q9GT24|ZYG1_CAEEL Probable serine/threonine-protein kinase zyg-1 (Zygote defective protein 1) 0.0
blast Q621J7 Probable serine/threonine-protein kinase zyg-1 (Zygote defective protein 1) emb|CAE59156.1| Hypothetical protein CBG02462 [Caenorhabditis briggsae] 0.0
blast AAB54253 Zygote defective: embryonic lethal protein 1 [Caenorhabditis elegans] ref|NP_495103.1| ZYGote defective: embryonic lethal family member (zyg-1) [Caenorhabditis elegans] gb|AAG15377.1| zygote defective-1 [Caenorhabditis elegans] sp|Q9GT24|ZYG1_CAEEL Probable serine/threonine-protein kinase zyg-1 (Zygote defective protein 1) 1.0E-139
blast AAB54253 Zygote defective: embryonic lethal protein 1 [Caenorhabditis elegans] ref|NP_495103.1| ZYGote defective: embryonic lethal family member (zyg-1) [Caenorhabditis elegans] gb|AAG15377.1| zygote defective-1 [Caenorhabditis elegans] sp|Q9GT24|ZYG1_CAEEL Probable serine/threonine-protein kinase zyg-1 (Zygote defective protein 1) 1.0E-134
blast T15269 hypothetical protein F59E12.1 - Caenorhabditis elegans 2.0E-95
blast T15269 hypothetical protein F59E12.1 - Caenorhabditis elegans 8.0E-95
Protein Families
Method ID Description E-Value
hanks 2.1.25 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; CDPK 9.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 5.9E-65
Sequence
Target Sequence 
>C. ELEGANS:188218_S_AT
gaatcgcgacccaaaaacaggcagtttctggaataacacttacaaaagtgaatgaagtat
ataaatatctaataagattcgagcaatgtcttaacggaatggatcgaggaatggtgtgct
ttcccattgttttcagtgcaggaactaatatggttggcagttcgccgtcttcattaatgc
catcaggatcttctcaaacttcccgatttccatttagtaatttatcgaacaatcagccat
cattagttccccattcggctccttttcttacgaaaccaacttcatctcaacgagcatcat
cggctaatgttcagagaagggtatcaactgatgaaaattcgtcacctagtgtggctccat
caaagtataaaataaaaattgaccctacaactcaaaaagttcgatcaattcaagcaacag
acggtcgtgtgctacgatgttccacaagtaaagcggatcaattcattttcacagatccgg
cgattcgtcctgatgatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCGCGACCCAAAAACAGGCAGT 480 329 1592 Antisense
GTGCTTTCCCATTGTTTTCAGTGCA 438 479 1707 Antisense
AATATGGTTGGCAGTTCGCCGTCTT 130 173 1738 Antisense
ATTAATGCCATCAGGATCTTCTCAA 149 13 1764 Antisense
AACAATCAGCCATCATTAGTTCCCC 324 133 1819 Antisense
ACCAACTTCATCTCAACGAGCATCA 456 85 1866 Antisense
ACGAGCATCATCGGCTAATGTTCAG 551 93 1881 Antisense
AAAATTCGTCACCTAGTGTGGCTCC 696 133 1925 Antisense
GACGGTCGTGTGCTACGATGTTCCA 588 375 2011 Antisense
AATTCATTTTCACAGATCCGGCGAT 551 173 2051 Antisense
ATCCGGCGATTCGTCCTGATGATCA 378 37 2066 Antisense
Sequence Source Affymetrix Proprietary Database