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[188215_at]

Description T07A9.6 /REP_DB=WormBase Gene ID /WP=CE26385 /GEN=daf-18 /TR=O44405 /GB=AAK39284.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 87      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 94      6grp_total > 78      4grp_total > 39      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 342.3
(3)
P 		276.8
(-0.5)
P 		233.8
(-2.8)
P 		230.6
(-3)
P 		112
B 409
(3)
P 		188.7
(-3)
P 		233.6
(-1.8)
P 		287
(-0.3)
P 		220
C 367.7
(3)
P 		189.9
(-3)
P 		229.2
(-1.7)
P 		295.7
(0.6)
P 		178
avr 373
(3) 		218.5
(-3) 		232.2
(-2.5) 		271.1
(-1) 		155

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1149
1 2 3 4 5 6 range
A 277.7
(-2.7)
P 		380.6
(-2.2)
P 		225
(-3)
P 		384
(-2.2)
P 		685.4
(-0.6)
P 		1373.8
(3)
P 		1149
B 327.5
(-2.9)
P 		332.4
(-2.9)
P 		311.8
(-3)
P 		389.1
(-2.5)
P 		650.9
(-1)
P 		1327
(3)
P 		1015
C 348.9
(-3)
P 		354.6
(-2.9)
P 		347.3
(-3)
P 		344.5
(-3)
P 		761.4
(-0.6)
P 		1366
(3)
P 		1022
avr 318
(-2.9) 		355.9
(-2.7) 		294.7
(-3) 		372.5
(-2.6) 		699.2
(-0.7) 		1355.6
(3) 		1061
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 124.6
(3)
P 		60.1
(-1.3)
P 		61.1
(-1.2)
P 		33.7
(-3)
P 		91
B 130.4
(3)
P 		46.6
(-3)
P 		54.1
(-2.5)
P 		61.2
(-2)
P 		84
C 121.7
(3)
P 		54.6
(-2.6)
P 		79.9
(-0.5)
P 		49.9
(-3)
P 		72
avr 125.6
(3) 		53.8
(-2.6) 		65
(-1.7) 		48.3
(-3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 446
1 2 3 4 5 6 range
A 51.2
(-3)
P 		135.4
(-1.2)
P 		49
(-3)
P 		97.4
(-2)
P 		206.8
(0.3)
P 		334.5
(3)
P 		286
B 114
(-2.5)
P 		110.6
(-2.5)
P 		75.7
(-3)
P 		103
(-2.6)
P 		264.4
(-0.3)
P 		494.8
(3)
P 		419
C 129.3
(-2.1)
P 		108.2
(-2.4)
P 		88.4
(-2.7)
P 		69.3
(-3)
P 		246.4
(-0.3)
P 		457.3
(3)
P 		388
avr 98.2
(-2.5) 		118.1
(-2.2) 		71
(-3) 		89.9
(-2.7) 		239.2
(-0.2) 		428.9
(3) 		358
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188215_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10423
Sequence Type Exemplar sequence
Representative Public ID T07A9.6 NCBI
Target Description T07A9.6 /REP_DB=WormBase Gene ID /WP=CE26385 /GEN=daf-18 /TR=O44405 /GB=AAK39284.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067525(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067525 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-18) (daf-18) mRNA, complete cds. 11/11 A 
SNAP00000017768 ENSEMBL cdna:SNAP chromosome:CEL140:IV:420426:425148:-1 11/11 None
GENEFINDER00000017770 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:420426:425148:-1 11/11 None
T07A9.6 ENSEMBL cdna:known chromosome:CEL140:IV:420013:425173:-1 gene:T07A9.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:420425-425148(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol daf-18
Ensembl T07A9.6
Entrez Gene 176869 Entrez gene
SwissProt O44405 EMBL-EBI
WormBase CE26385 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6470 protein amino acid dephosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4721 phosphoprotein phosphatase activity inferred from electronic annotation QuickGO AmiGO
4725 protein tyrosine phosphatase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK39284 Abnormal dauer formation protein 18 [Caenorhabditis elegans] ref|NP_499926.1| abnormal DAuer Formation family member (daf-18) [Caenorhabditis elegans] emb|CAA10315.1| DAF-18 protein [Caenorhabditis elegans] gb|AAD21620.1| PTEN phosphatidylinositol 3' phosphatase homolog DAF-18 [Caenorhabditis elegans] gb|AAD03420.1| DAF-18 [Caenorhabditis elegans] 0.0
blast T32574 hypothetical protein T07A9.6 - Caenorhabditis elegans 0.0
blast CAE67940 Hypothetical protein CBG13540 [Caenorhabditis briggsae] 1.0E-178
Sequence
Target Sequence 
>C. ELEGANS:188215_AT
gaaccactcgaacatctattccatgaatcataccatccaaatacggccggtaacatgctg
cgtcaggattatcacactgattcggaagtgaaaatagctgaacaagaggcaaaagccttc
gttgaccagttgcttaatggacaaggtgtattacaagagtttatgaagcaattcaaagta
ccatcggacaattcctttgctgattatgtaaccggacaggccgaagtttttaaagcacag
attgcgttactggagcagtcggaggattttcaacgagttcaagcgaatgcagaggaagtc
gatcttgaacacactcttggtgaagcgtttgagcgattcgggcacgttgtagaagaatcg
aatggttcttctaaaaatccaaaagccctgaaaactcgagaacaaatggtgaaagaaact
ggcaaagacactcagaagacccgcaatcatgtgcttctaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACCACTCGAACATCTATTCCATG 78 345 2299 Antisense
GAATCATACCATCCAAATACGGCCG 494 327 2323 Antisense
AATACGGCCGGTAACATGCTGCGTC 311 171 2338 Antisense
ATGCTGCGTCAGGATTATCACACTG 282 43 2353 Antisense
GAGGCAAAAGCCTTCGTTGACCAGT 6 405 2404 Antisense
GTACCATCGGACAATTCCTTTGCTG 585 457 2476 Antisense
ATTCCTTTGCTGATTATGTAACCGG 594 7 2489 Antisense
GAAGTCGATCTTGAACACACTCTTG 125 337 2593 Antisense
AGCGTTTGAGCGATTCGGGCACGTT 522 81 2622 Antisense
AAAGACACTCAGAAGACCCGCAATC 598 121 2722 Antisense
AGACCCGCAATCATGTGCTTCTACA 264 71 2735 Antisense
Sequence Source Affymetrix Proprietary Database