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[188203_at]

Description T27A3.3 /REP_DB=WormBase Gene ID /WP=CE14221 /GEN=ssp-16 /TR=P91499 /GB=AAB42295.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 33      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 26.2
(0)
A 		38.1
(3)
A 		22
(-1)
A 		14
(-3)
A 		24
B 10.8
(-3)
A 		19.9
(-1.8)
A 		54.6
(3)
A 		39.2
(0.9)
A 		44
C 15.6
(-2.8)
A 		34.2
(0.1)
A 		14.6
(-3)
A 		52.4
(3)
A 		38
avr 17.5
(-3) 		30.7
(1.5) 		30.4
(1.4) 		35.2
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 725
1 2 3 4 5 6 range
A 29.4
(-2.8)
A 		7.3
(-3)
A 		11.4
(-3)
A 		152
(-1.8)
P 		618.1
(2.1)
P 		729
(3)
P 		722
B 28.9
(-2.8)
A 		24.2
(-2.8)
A 		4.4
(-3)
A 		153.2
(-1.7)
P 		678
(3)
P 		648.6
(2.7)
P 		674
C 5.7
(-3)
A 		44.7
(-2.7)
A 		49.3
(-2.6)
A 		149.5
(-1.8)
P 		571.4
(1.8)
P 		712.6
(3)
P 		707
avr 21.3
(-3) 		25.4
(-3) 		21.7
(-3) 		151.6
(-1.8) 		622.5
(2.3) 		696.7
(3) 		675
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 9.5
(3)
A 		8.3
(2)
A 		5.8
(0)
A 		2
(-3)
A 		8
B 3.4
(-3)
A 		4.9
(-2)
A 		12.7
(3)
A 		8.4
(0.2)
A 		9
C 5.1
(-3)
A 		9.8
(3)
A 		5.1
(-3)
A 		8.8
(1.7)
A 		5
avr 6
(-3) 		7.7
(2.4) 		7.9
(3) 		6.4
(-1.7) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 274
1 2 3 4 5 6 range
A 5.4
(-2.9)
A 		2.6
(-3)
A 		2.5
(-3)
A 		38.6
(-1.8)
P 		186.5
(3)
P 		177.5
(2.7)
P 		184
B 10
(-2.8)
A 		8.1
(-2.8)
A 		1.1
(-3)
A 		40.6
(-2.1)
P 		275.5
(3)
P 		241.8
(2.3)
P 		274
C 2.1
(-3)
A 		13.7
(-2.7)
A 		12.5
(-2.7)
A 		30.1
(-2.3)
P 		184.9
(1.6)
P 		238.5
(3)
P 		236
avr 5.8
(-3) 		8.1
(-2.9) 		5.4
(-3) 		36.4
(-2.1) 		215.6
(2.9) 		219.3
(3) 		214
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188203_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1775
Sequence Type Exemplar sequence
Representative Public ID T27A3.3 NCBI
Target Description T27A3.3 /REP_DB=WormBase Gene ID /WP=CE14221 /GEN=ssp-16 /TR=P91499 /GB=AAB42295.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059361(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059361 NCBI Caenorhabditis elegans Sperm-Specific family, class P family member (ssp-16) (ssp-16) mRNA, complete cds. 11/11 None
SNAP00000022768 ENSEMBL cdna:SNAP chromosome:CEL140:I:6110202:6110531:1 11/11 None
GENEFINDER00000022781 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6110202:6110531:1 11/11 None
T27A3.3 ENSEMBL cdna:known chromosome:CEL140:I:6110202:6110531:1 gene:T27A3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6110201-6110531(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ssp-16
Ensembl T27A3.3
Entrez Gene 172294 Entrez gene
SwissProt P91499 EMBL-EBI
WormBase CE14221 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42295 Sperm-specific family, class p protein 16 [Caenorhabditis elegans] ref|NP_491762.1| Sperm-Specific family, class P family member (ssp-16) [Caenorhabditis elegans] 1.0E-42
blast CAA94274 Hypothetical protein K07F5.9 [Caenorhabditis elegans] ref|NP_501767.1| Sperm-Specific family, class P family member (ssp-10) [Caenorhabditis elegans] 2.0E-41
blast CAE59021 Hypothetical protein CBG02298 [Caenorhabditis briggsae] 3.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 6.3E-34
Sequence
Target Sequence 
>C. ELEGANS:188203_AT
gctcctctactcacaagcttgttaacggaggtgccgataagctcatcttcaagatcaagt
catcaaacaacaatgagtaccgtattgtcccagtctttggatttgtcgacccatctggat
ccaaggacgtcgtgatcactcgcactgctggagcaccaaaggaggacaaacttgtcgttc
atttcgcacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCCTCTACTCACAAGCTTGTTAA 355 297 62 Antisense
ACAAGCTTGTTAACGGAGGTGCCGA 176 111 74 Antisense
GGAGGTGCCGATAAGCTCATCTTCA 635 515 88 Antisense
GCCGATAAGCTCATCTTCAAGATCA 67 285 94 Antisense
AATGAGTACCGTATTGTCCCAGTCT 439 165 133 Antisense
ACCGTATTGTCCCAGTCTTTGGATT 376 87 140 Antisense
ATTGTCCCAGTCTTTGGATTTGTCG 133 3 145 Antisense
CGACCCATCTGGATCCAAGGACGTC 84 251 168 Antisense
TCCAAGGACGTCGTGATCACTCGCA 596 587 181 Antisense
GGACAAACTTGTCGTTCATTTCGCA 75 527 225 Antisense
ACAAACTTGTCGTTCATTTCGCACC 43 113 227 Antisense
Sequence Source Affymetrix Proprietary Database