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[188200_at]

Description T14B4.7 /REP_DB=WormBase Gene ID /WP=CE04954 /GEN=dpy-10 /TR=Q22476 /GB=AAK31556.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 0      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 21      6grp_total > 03      4grp_total > 49      6grp_cgc > 50      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2761
1 2 3 4 range
A 143.8
(-3)
P 		2725.5
(3)
P 		2501.9
(2.5)
P 		204
(-2.9)
P 		2582
B 169.2
(-2.9)
P 		2633.9
(2.5)
P 		2868.5
(3)
P 		107.3
(-3)
P 		2761
C 185.3
(-3)
P 		2559
(2.6)
P 		2744.5
(3)
P 		167.6
(-3)
P 		2577
avr 166.1
(-3) 		2639.5
(2.8) 		2705
(3) 		159.6
(-3) 		2545

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1802
1 2 3 4 5 6 range
A 1506.9
(1.4)
P 		1864.2
(3)
P 		975.7
(-1.1)
P 		1428.4
(1)
P 		735.2
(-2.2)
P 		558.9
(-3)
P 		1305
B 1477.2
(3)
P 		1375.6
(2.4)
P 		948.6
(-0.1)
P 		1224.1
(1.5)
P 		816.5
(-0.9)
P 		459.7
(-3)
P 		1018
C 2013.8
(2.5)
P 		1210.6
(-0.2)
P 		1265.3
(-0)
P 		2177.8
(3)
P 		1127.3
(-0.5)
P 		375.9
(-3)
P 		1802
avr 1666
(3) 		1483.5
(2.1) 		1063.2
(-0) 		1610.1
(2.7) 		893
(-0.9) 		464.8
(-3) 		1201
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 934
1 2 3 4 range
A 52.4
(-2.8)
P 		591.6
(2.4)
P 		654.1
(3)
P 		29.9
(-3)
P 		624
B 54
(-2.7)
P 		650.5
(2.9)
P 		664.7
(3)
P 		22.9
(-3)
P 		642
C 61.3
(-2.8)
P 		735.7
(1.6)
P 		956.5
(3)
P 		28.3
(-3)
P 		928
avr 55.9
(-2.8) 		659.3
(2.2) 		758.4
(3) 		27
(-3) 		731

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 621
1 2 3 4 5 6 range
A 277.8
(-1.4)
P 		663
(3)
P 		212.5
(-2.1)
P 		362.3
(-0.4)
P 		221.9
(-2)
P 		136.1
(-3)
P 		527
B 514
(3)
P 		457.9
(2)
P 		230.4
(-2)
P 		324.2
(-0.3)
P 		331.7
(-0.2)
P 		171.4
(-3)
P 		343
C 746.4
(3)
P 		369.4
(-0.6)
P 		322
(-1.1)
P 		437.9
(0)
P 		364.8
(-0.7)
P 		125.8
(-3)
P 		621
avr 512.7
(3) 		496.8
(2.7) 		255
(-1.2) 		374.8
(0.8) 		306.1
(-0.4) 		144.4
(-3) 		368
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188200_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5484
Sequence Type Exemplar sequence
Representative Public ID T14B4.7 NCBI
Target Description T14B4.7 /REP_DB=WormBase Gene ID /WP=CE04954 /GEN=dpy-10 /TR=Q22476 /GB=AAK31556.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062965(11), NM_182248(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062965 NCBI Caenorhabditis elegans DumPY : shorter than wild-type family member (dpy-10) (dpy-10) mRNA, complete cds. 11/11 None
NM_182248 NCBI Caenorhabditis elegans DumPY : shorter than wild-type family member (dpy-10) (dpy-10) mRNA, complete cds. 11/11 None
SNAP00000000073 ENSEMBL cdna:SNAP chromosome:CEL140:II:6710306:6712621:-1 11/11 None
GENEFINDER00000000088 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6710306:6714006:-1 11/11 None
T14B4.7a.2 ENSEMBL cdna:known chromosome:CEL140:II:6709778:6712174:-1 gene:T14B4.7 11/11 A 
T14B4.7a.1 ENSEMBL cdna:known chromosome:CEL140:II:6710100:6712169:-1 gene:T14B4.7 11/11 A 
T14B4.7b.1 ENSEMBL cdna:known chromosome:CEL140:II:6710306:6712875:-1 gene:T14B4.7 11/11 None
T14B4.7b.2 ENSEMBL cdna:known chromosome:CEL140:II:6710306:6712170:-1 gene:T14B4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6710504-6711407(-) 100.0 100.0
Public Domain and Genome References
Ensembl T14B4.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_872048 DumPY : shorter than wild-type family member (dpy-10) [Caenorhabditis elegans] gb|AAO38600.2| Dumpy : shorter than wild-type protein 10, isoform b [Caenorhabditis elegans] 7.0E-88
blast AAA17395 collagen [Caenorhabditis elegans] sp|P35800|DPY10_CAEEL Cuticle collagen dpy-10 precursor (Dumpy-10 protein) 1.0E-74
blast AAA17395 collagen [Caenorhabditis elegans] sp|P35800|DPY10_CAEEL Cuticle collagen dpy-10 precursor (Dumpy-10 protein) 3.0E-73
blast NP_872048 DumPY : shorter than wild-type family member (dpy-10) [Caenorhabditis elegans] gb|AAO38600.2| Dumpy : shorter than wild-type protein 10, isoform b [Caenorhabditis elegans] 5.0E-70
blast AAA17395 collagen [Caenorhabditis elegans] sp|P35800|DPY10_CAEEL Cuticle collagen dpy-10 precursor (Dumpy-10 protein) 1.0E-65
blast NP_495366 DumPY : shorter than wild-type family member (dpy-10) [Caenorhabditis elegans] gb|AAK31556.2| Dumpy : shorter than wild-type protein 10, isoform a [Caenorhabditis elegans] 4.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 0.0018
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.5E-10
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.9E-9
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 0.0018
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.5E-10
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.9E-9
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 0.0018
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.5E-10
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.9E-9
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 0.0018
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.5E-10
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.9E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495366.2 1 7-29
NP_872048.2 1 27-49
T14B4.7a.2 1 7-29
T14B4.7a.1 1 7-29
T14B4.7b.1 1 27-49
T14B4.7b.2 1 27-49
SNAP00000000073 1 122-144
GENEFINDER00000000088 1 94-116
Sequence
Target Sequence 
>C. ELEGANS:188200_AT
gcttcacatggcttcagaccaacagcctatggagatgaaatcactggagctccacttgaa
actgaatgtccaggatgttgcattccaggaccaccaggtccacgtggatcatctggaaca
ccaggaaaacctggacttccaggaaacgctggaaagccaggaatgccaggaactactcca
aatcaaacatgcccactcaaccaagttcgtgagccaccaccgtgccgtccatgtccaaag
ggaccaccaggaatcaagggatggccaggattcccaggagatgttgggccaccaggacca
ccaggacttaagggaattgatggtgaagacggagcaccaggagagactggaccaactgga
ccaccaggatacagaggaggaccaggagctcctggagataagggaccaactccagaaggt
gacttgaaggagggaccaccaggagacgaaggaccaccaggaccaattggagctccagga
atgcctggattaccaggaagaaacggattgaccggaggacaaggagaacgaggatggccg
ggagtttctggaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTCACATGGCTTCAGACCAACAG 615 305 244 Antisense
GGAGCTCCACTTGAAACTGAATGTC 648 521 289 Antisense
CAGGATGTTGCATTCCAGGACCACC 331 209 314 Antisense
ACCACCAGGTCCACGTGGATCATCT 480 85 333 Antisense
GGAATGCCAGGAACTACTCCAAATC 51 533 403 Antisense
CTACTCCAAATCAAACATGCCCACT 439 225 416 Antisense
GCCCACTCAACCAAGTTCGTGAGCC 100 287 434 Antisense
GTGCCGTCCATGTCCAAAGGGACCA 654 477 465 Antisense
GATTCCCAGGAGATGTTGGGCCACC 38 431 512 Antisense
GGGACCAACTCCAGAAGGTGACTTG 337 487 645 Antisense
GAGGATGGCCGGGAGTTTCTGGAGA 24 405 773 Antisense
Sequence Source Affymetrix Proprietary Database